蔗糖、水杨酸和钾营养对彩色马铃薯试管薯结薯效率的影响
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摘要
【目的】提高彩色马铃薯试管薯结薯效率,降低生产成本,为工厂化生产试管薯提供依据。【方法】以8个彩色马铃薯株系为供试材料,研究12%蔗糖、0.5 mmol/L水杨酸、40 mmol/L高钾处理对彩色马铃薯试管薯结薯的影响。【结果】12%蔗糖处理可提高单株试管薯产量和淀粉含量。0.5mmol/L水杨酸处理使不同株系增产19%～192%,但淀粉含量略有下降。40mmol/L高钾处理对试管薯产量和淀粉含量作用不显著或抑制少量株系试管薯形成并降低淀粉含量。此外,水杨酸具有促进结薯提前的作用,可比对照平均提前约15～25 d形成直径3～5 mm薯块。生产1 kg彩色马铃薯试管薯,0.5 mmol/L水杨酸处理的试剂成本最低,只有对照的56.89%。【结论】0.5 mmol/L水杨酸处理具有成本低、结薯效率高的优点,具有实际应用潜力。
【Objective】In order to improve the tuberization efficiency of pigmented potato in vitro,reduce production cost and provide basis for factory production of microtuber.【Method】Eight pigmented potato lines were used as tested materials to study the effects of 12% sucrose,0.5 mmol/L salicylic acid(SA)and 40 mmol/L high potassium treatment on the microtuber tuberization.【Result】12% sucrose treatment could increase the single plant yield of microtuber and starch content. 0.5 mmol/L SA treatment increased the yield of different lines by 19%-192%,but the starch content decreased slightly.40 mmol/L high potassium treatment had no significant effect on the yield and starch content of microtuber,or inhibited the microtuber formation of a few lines and decreased the starch content. In addition,SA had the effect of promoting the early tuber growth,which was averagely about 15-25 days ahead of the control,forming a diameter of 3-5 mm potato tuber. The reagent cost of 0.5 mmol/L SA treatment was the lowest for producing 1 kg microtubers,only 56.89% of the control.【Conclusion】0.5 mmol/L SA treatment has the advantages of low cost and high tuberization efficiency,and has practical application potential.
【Objective】In order to improve the tuberization efficiency of pigmented potato in vitro,reduce production cost and provide basis for factory production of microtuber.【Method】Eight pigmented potato lines were used as tested materials to study the effects of 12% sucrose,0.5 mmol/L salicylic acid(SA)and 40 mmol/L high potassium treatment on the microtuber tuberization.【Result】12% sucrose treatment could increase the single plant yield of microtuber and starch content. 0.5 mmol/L SA treatment increased the yield of different lines by 19%-192%,but the starch content decreased slightly.40 mmol/L high potassium treatment had no significant effect on the yield and starch content of microtuber,or inhibited the microtuber formation of a few lines and decreased the starch content. In addition,SA had the effect of promoting the early tuber growth,which was averagely about 15-25 days ahead of the control,forming a diameter of 3-5 mm potato tuber. The reagent cost of 0.5 mmol/L SA treatment was the lowest for producing 1 kg microtubers,only 56.89% of the control.【Conclusion】0.5 mmol/L SA treatment has the advantages of low cost and high tuberization efficiency,and has practical application potential.
引文
[1]黄凤玲,张琳,李先德,等.中国马铃薯产业发展现状及对策[J].农业展望,2017,13(1):25-31.
[2]杨雅伦,郭燕枝,孙君茂.我国马铃薯产业发展现状及未来展望[J].中国农业科技导报,2017,19(1):29-36.
[3]LUIS F SALAZAR.马铃薯病毒及其防治[M].阎文昭,张勇飞,等译.北京:中国农业科技出版社,2001:183-184.
[4]胡琳.植物脱毒技术[M].北京:中国农业大学出版社,2000:111-112.
[5]刘尚前,王晓春,栗占芳,等.马铃薯试管薯形成的影响因素研究[J].西北农业学报,2007,16(5):109-112.
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[8]帅正彬,郭江洪,杨斌,等.不同培养条件对马铃薯试管薯诱导的影响[J].西南农业学报,2004,17(2):212-214.
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[10]包丽仙,李山云,杨琼芬,等.引进彩色马铃薯资源的农艺性状及块茎性状评价[J].西南农业学报,2012,25(4):1187-1192.
[11]BROWN C R,WROLSTAD R,DURST R,et al.Breeding studies in potatoes containing high concentrations of anthocyanins[J].American Journal of Potato Research,2003,80(4):241-249.
[12]张艳萍,裴怀弟,石有太,等.大量元素的浓度改变对彩色马铃薯试管薯诱导的影响[J].江苏农业科学,2014,42(12):38-40.
[13]李婉琳,郭华春,彭丽,等.蔗糖浓度及苗龄对马铃薯新品种‘丽薯6号’试管薯诱导的效果[J].中国马铃薯,2013,27(2):72-76.
[14]李灿辉,王军,龙维彪.马铃薯块茎特异蛋白Patatin研究进展[J].马铃薯杂志,1998,12(3):179-186.
[15]黄雪丽,胡应锋,邹雪,等.钾高效基因型马铃薯钾素利用效率机制研究[J].西南农业学报,2013,26(3):1094-1099.
[16]韩德俊,陈耀锋,李春莲,等.水杨酸对马铃薯试管微薯形成的影响研究[J].西北植物学报,1999,19(3):428-433.
[17]贺静,陈屹,张云孙.马铃薯试管薯的诱导[J].云南大学学报(自然科学版),2001,23(1):62-64.
[18]邹雪,肖乔露,文安东,等.通过体细胞无性系变异获得马铃薯优良新材料[J].园艺学报,2015,42(3):480-488.
[19]邹雪.StGAPC和AtGAPC2基因的克隆以及对马铃薯的转化[D].雅安:四川农业大学,2011.
[20]项洪涛.三种植物生长调节剂对马铃薯碳代谢生理及产量品质的影响[D].大庆:黑龙江八一农垦大学,2013.
[21]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:30-31,81-83.
[22]宁志珩,吕国华,贾晓鹰.脱毒马铃薯试管薯诱导技术探索[J].中国马铃薯,2007,21(1):33-38.
[23]韩德俊,陈耀锋,王亚娟,等.水杨酸和不同糖浓度对马铃薯试管微薯形成与生长的影响研究[J].西北植物学报,1999,19(6):92-96.
[24]NISTOR A,CHIRU N,CIOLOCA M,et al.Potato microtuberisation under the influence of certain organic acids[J].Studia Universitatis Vasile Goldis Seria Stiintele Vietii,2013,23(3):373-379.
[25]陈大清,王雪英,李亚男.水杨酸和茉莉酸甲酯对试管马铃薯形成的影响[J].华中农业大学学报,2005,24(1):74-78.
[26]KODA Y,TAKAHASHI K,KIKUTA Y.Potato tuber-inducing activities of salicylic acid and related compounds[J].Journal of Plant Growth Regulation,1992,11(4):215-219.
[27]张小微.茉莉酸调控的二倍体马铃薯离体块茎发育差异蛋白质组学分析[D].兰州:甘肃农业大学,2017.
[28]BAZABAKANA R,WATTIEZ R,BAUCHER M,et al.Effect of jasmonic acid on developmental morphology during in vitro tuberization of Dioscorea alata(L).[J].Plant Growth Regulation,2003,40(3):229-237.
[29]KU A T,HUANG Y S,WANG Y S,et al.IbMADS1(Ipomoea batatas MADS-box 1 gene)is involved in tuberous root initiation in sweet potato(Ipomoea batatas)[J].Annals of Botany,2008,102(1):57-67.
[30]李亚男,陈大清.水杨酸对甘薯的生理效应和块根产量的影响[J].湖北农学院学报,1996,16(3):190-193.
[31]殷文,孙春明,马晓燕,等.钾肥不同用量对马铃薯产量及品质的效应[J].中国土壤与肥料,2005(4):44-47.
[2]杨雅伦,郭燕枝,孙君茂.我国马铃薯产业发展现状及未来展望[J].中国农业科技导报,2017,19(1):29-36.
[3]LUIS F SALAZAR.马铃薯病毒及其防治[M].阎文昭,张勇飞,等译.北京:中国农业科技出版社,2001:183-184.
[4]胡琳.植物脱毒技术[M].北京:中国农业大学出版社,2000:111-112.
[5]刘尚前,王晓春,栗占芳,等.马铃薯试管薯形成的影响因素研究[J].西北农业学报,2007,16(5):109-112.
[6]VANDER ZAAG D E.Recent trend in development,production and utilization of potato crop in the world[J].APA Proceedings,1988,1(1):12-19.
[7]王炳君.马铃薯茎尖脱毒与微型薯生产[M].北京:高等教育出版社,1990:93-95.
[8]帅正彬,郭江洪,杨斌,等.不同培养条件对马铃薯试管薯诱导的影响[J].西南农业学报,2004,17(2):212-214.
[9]ESTRADA R,TOVAR P,DODDS J H.Induction of in vitro tubers in a broad range of potato genotypes[J].Plant Cell Tissue and Orange Culture,1986,7(1):3-10.
[10]包丽仙,李山云,杨琼芬,等.引进彩色马铃薯资源的农艺性状及块茎性状评价[J].西南农业学报,2012,25(4):1187-1192.
[11]BROWN C R,WROLSTAD R,DURST R,et al.Breeding studies in potatoes containing high concentrations of anthocyanins[J].American Journal of Potato Research,2003,80(4):241-249.
[12]张艳萍,裴怀弟,石有太,等.大量元素的浓度改变对彩色马铃薯试管薯诱导的影响[J].江苏农业科学,2014,42(12):38-40.
[13]李婉琳,郭华春,彭丽,等.蔗糖浓度及苗龄对马铃薯新品种‘丽薯6号’试管薯诱导的效果[J].中国马铃薯,2013,27(2):72-76.
[14]李灿辉,王军,龙维彪.马铃薯块茎特异蛋白Patatin研究进展[J].马铃薯杂志,1998,12(3):179-186.
[15]黄雪丽,胡应锋,邹雪,等.钾高效基因型马铃薯钾素利用效率机制研究[J].西南农业学报,2013,26(3):1094-1099.
[16]韩德俊,陈耀锋,李春莲,等.水杨酸对马铃薯试管微薯形成的影响研究[J].西北植物学报,1999,19(3):428-433.
[17]贺静,陈屹,张云孙.马铃薯试管薯的诱导[J].云南大学学报(自然科学版),2001,23(1):62-64.
[18]邹雪,肖乔露,文安东,等.通过体细胞无性系变异获得马铃薯优良新材料[J].园艺学报,2015,42(3):480-488.
[19]邹雪.StGAPC和AtGAPC2基因的克隆以及对马铃薯的转化[D].雅安:四川农业大学,2011.
[20]项洪涛.三种植物生长调节剂对马铃薯碳代谢生理及产量品质的影响[D].大庆:黑龙江八一农垦大学,2013.
[21]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:30-31,81-83.
[22]宁志珩,吕国华,贾晓鹰.脱毒马铃薯试管薯诱导技术探索[J].中国马铃薯,2007,21(1):33-38.
[23]韩德俊,陈耀锋,王亚娟,等.水杨酸和不同糖浓度对马铃薯试管微薯形成与生长的影响研究[J].西北植物学报,1999,19(6):92-96.
[24]NISTOR A,CHIRU N,CIOLOCA M,et al.Potato microtuberisation under the influence of certain organic acids[J].Studia Universitatis Vasile Goldis Seria Stiintele Vietii,2013,23(3):373-379.
[25]陈大清,王雪英,李亚男.水杨酸和茉莉酸甲酯对试管马铃薯形成的影响[J].华中农业大学学报,2005,24(1):74-78.
[26]KODA Y,TAKAHASHI K,KIKUTA Y.Potato tuber-inducing activities of salicylic acid and related compounds[J].Journal of Plant Growth Regulation,1992,11(4):215-219.
[27]张小微.茉莉酸调控的二倍体马铃薯离体块茎发育差异蛋白质组学分析[D].兰州:甘肃农业大学,2017.
[28]BAZABAKANA R,WATTIEZ R,BAUCHER M,et al.Effect of jasmonic acid on developmental morphology during in vitro tuberization of Dioscorea alata(L).[J].Plant Growth Regulation,2003,40(3):229-237.
[29]KU A T,HUANG Y S,WANG Y S,et al.IbMADS1(Ipomoea batatas MADS-box 1 gene)is involved in tuberous root initiation in sweet potato(Ipomoea batatas)[J].Annals of Botany,2008,102(1):57-67.
[30]李亚男,陈大清.水杨酸对甘薯的生理效应和块根产量的影响[J].湖北农学院学报,1996,16(3):190-193.
[31]殷文,孙春明,马晓燕,等.钾肥不同用量对马铃薯产量及品质的效应[J].中国土壤与肥料,2005(4):44-47.