长角血蜱野外种群对常用杀虫剂相对敏感基线和诊断剂量研究
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摘要
目的建立长角血蜱野外种群对常用杀虫剂的相对敏感基线和诊断剂量。方法分别于2013年和2014年4-9月采集河南省王屋山区未接触过任何杀虫剂的长角血蜱野外种群。采用药膜法测定长角血蜱成蜱对杀虫剂的敏感性。采用DPS数据处理软件进行机值分析,拟合毒力回归方程,确定半数致死剂量、99%致死剂量,计算诊断剂量。结果建立了对敌敌畏、双硫磷、仲丁威、氯菊酯、溴氰菊酯和高效氯氰菊酯6种杀虫剂的相对敏感基线,分别为y=1.549 9+1.359 4x、y=2.343 3+1.225 6x、y=-6.850 7+5.213 9x、y=0.866 1+1.571 1x、y=0.411 6+2.277 8x和y=2.551 8+1.018 9x;相应的诊断剂量分别为35.505 6、10.764 7、1.047 4、25.872 9、2.171 4和97.063 2 mg/L。结论建立了长角血蜱野外种群对常用杀虫剂的相对敏感基线和诊断剂量,可作为长角血蜱抗药性监测的参考。
Objective To establish the relative susceptibility baselines and diagnostic doses of the wild population of Haemaphysalis longicornis to common insecticides. Methods The wild population of H. longicornis without exposure to any pesticides was collected at Mount Wangwu in Henan, China from April to September in both 2013 and 2014. The residual film method was used to determine the susceptibility of adult H. longicornis to insecticides. DPS data processing software was used for probit analysis, and the toxicity regression equation was fitted to determine the median lethal dose and99% lethal dose and then to calculate the diagnostic dose. Results The susceptibility baselines of H. longicornis to dichlorvos,temephos, bassa, permethrin, deltamethrin, and beta-cypermethrin were y= 1.549 9+ 1.359 4 x, y=2.343 3+ 1.225 6 x,y=-6.850 7 + 5.213 9 x, y=0.866 1 + 1.571 1 x, y=0.411 6 + 2.277 8 x, and y=2.551 8+ 1.018 9 x, respectively; the diagnostic doses were 35.505 6, 10.764 7, 1.047 4, 25.872 9, 2.171 4, and 97.063 2 mg/L, respectively. Conclusion The relative susceptibility baselines of the wild population of H. longicornis to the six insecticides, as well as the diagnostic doses, have been established. The results can be used as a reference for monitoring the resistance of H. longicornis to pesticides.
Objective To establish the relative susceptibility baselines and diagnostic doses of the wild population of Haemaphysalis longicornis to common insecticides. Methods The wild population of H. longicornis without exposure to any pesticides was collected at Mount Wangwu in Henan, China from April to September in both 2013 and 2014. The residual film method was used to determine the susceptibility of adult H. longicornis to insecticides. DPS data processing software was used for probit analysis, and the toxicity regression equation was fitted to determine the median lethal dose and99% lethal dose and then to calculate the diagnostic dose. Results The susceptibility baselines of H. longicornis to dichlorvos,temephos, bassa, permethrin, deltamethrin, and beta-cypermethrin were y= 1.549 9+ 1.359 4 x, y=2.343 3+ 1.225 6 x,y=-6.850 7 + 5.213 9 x, y=0.866 1 + 1.571 1 x, y=0.411 6 + 2.277 8 x, and y=2.551 8+ 1.018 9 x, respectively; the diagnostic doses were 35.505 6, 10.764 7, 1.047 4, 25.872 9, 2.171 4, and 97.063 2 mg/L, respectively. Conclusion The relative susceptibility baselines of the wild population of H. longicornis to the six insecticides, as well as the diagnostic doses, have been established. The results can be used as a reference for monitoring the resistance of H. longicornis to pesticides.
引文
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[2]刘吉起,郭祥树,高平,等.安阳鹤壁两地区蜱对不同宿主侵害状况的调查研究[J].中华卫生杀虫药械,2011,17(5):383-384.
[3]曹允.蜱的危害及防治措施研究进展[J].山东畜牧兽医,2015,36(2):56-58. DOI:10.3969/j.issn.1007-1733.2015.02.041.
[4] Barriga OO. A review on vaccination against protozoa andarthropods of veterinary importance[J]. Vet Parasitol,1994,55(1/2):29-55.DOI:10.1016/0304-4017(94)90054-X.
[5]石淑珍,刘增加,杨银书,等.复方高效灭蜱剂灭蜱药效研究[J].中华卫生杀虫药械,2006,12(3):187-188. DOI:10.3969/j.issn.1671-2781.2006.03.011.
[6]陈式明,唐仕雄,徐海涛,等.某战备仓库蜱虫危害调查与控制[J].中国媒介生物学及控制杂志,2012,23(2):169-171.
[7]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB/T 26352-2010蜚蠊抗药性检测方法德国小蠊生物测定法[S].北京:中国标准出版社,2011.
[8]鲁艳辉,杨婷,高希武.禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立[J].昆虫学报,2009,52(1):52-58.DOI:10.3321/j.issn:0454-6296.2009.01.008.
[9]刘吉起,赵奇,许汴利.蜱类研究进展[J].中国媒介生物学及控制杂志,2013,24(2):186-188.
[10]甘德礼.光山县2009-2011年发热伴血小板减少综合征发病情况分析[J].医药论坛杂志,2012,33(10):52-53.
[11]刘洋,黄学勇,杜燕华,等.河南发热伴血小板减少综合征流行区蜱类分布及媒介携带新布尼亚病毒状况调查[J].中华预防医学杂志,2012,46(6):500-504. DOI:10.3760/cma.j.issn.0253-9624.2012.06.005.