Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model
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摘要
Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera(M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds(100 and 200 mg/kg), two groups with propolis(50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone(reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GCMS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended.
Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera(M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds(100 and 200 mg/kg), two groups with propolis(50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone(reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GCMS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended.
Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera(M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds(100 and 200 mg/kg), two groups with propolis(50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone(reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GCMS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended.
引文
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[2]Fukuda T,Naganuma M,Kanai T.Current new challenges in the management of ulcerative colitis.Intest Res 2019;17(1):36-44.Doi:10.5217/ir.2018.00126.
[3]Kumar VL,Pandey A,Verma S,Das P.Protection afforded by methanol extract of Calotropis procera latex in experimental model of colitis is mediated through inhibition of oxidative stress and pro-inflammatory signaling.Biomed Pharmacother 2019;109:1602-1609.Doi:10.1016/j.biopha.2018.
[4]Deleporte A,Viennot S,Dupont B,Giletta C,Allaire M,Prévost F,et al.Efficacy of anti-TNF-alpha monoclonal antibodies in inflammatory bowel disease treatment.Int J Interferon Cytokine Mediat Res 2013;2013(5):11-31.Doi:10.2147/IJICMR.S21705.
[5]Bilsborough J,Targan SR,Snapper SB.Therapeutic targets in inflammatory bowel disease:Current and future.Am J Gastroenterol Suppl2016;3:27-37.Doi:10.1038/ajgsup.2016.18.
[6]Stucker F,Ackermann D.Immunosuppressive drugs-how they work,their side effects and interactions.Ther Umsch 2011;68(12):679-686.Doi:10.1024/0040-5930/a000230.
[7]Padayachee B,Baijnath H.An overview of the medicinal importance of Moringaceae.J Med Plants Res 2012;6(48):5831-5839.Doi:10.5897/JMPR12.1187.
[8]Mahajan SG,Mali RG,Mehta AA.Effect of Moringa oleifera Lam.seed extract on toluene diisocyanate-induced immune-mediated inflammatory responses in rats.J Immunotoxicol 2007;4(2):85-96.Doi:10.1080/15476910701337472.
[9]Coriolano MC,de Santana Brito J,de Siqueira Patriota LL,de Araujo Soares AK,Lorena VMB,Paiva PMG,et al.Immunomodulatory effects of the water-soluble lectin from Moringa oleifera seeds(WSMo L)on human peripheral blood mononuclear cells(PBMC).Protein Pept lett2018;25(3):295-301.Doi:10.2174/0929866525666180130141736.
[10]Stohs SJ,Hartman MJ.Review of the safety and efficacy of Moringa oleifera.Phytother Res 2015;29(6):796-804.Doi:10.1002/ptr.5325.
[11]Choudhary MK,Bodakhe SH,Gupta SK.Assessment of the antiulcer potential of Moringa oleifera root-bark extract in rats.J Acupunct Meridian Stud 2013;6(4):214-220.Doi:10.1016/j.jams.2013.07.003.
[12]Ijioma SN,Nwaogazi EN,Nwankwo AA,Oshilonya H,Ekeleme CM,Oshilonya LU.Histological exhibition of the gastroprotective effect of Moringa oleifera leaf extract.Comp Clin Pathol 2018;27(2):327-332.Doi:10.1007/s00580-017-2594-0.
[13]Machado BA,Silva RP,Barreto Gde A,Costa SS,Silva DF,Brand?o HN,et al.Chemical composition and biological activity of extracts obtained by supercritical extraction and ethanolic extraction of brown,green and red propolis derived from different geographic regions in Brazil.PLo SOne 2016;11(1):e0145954.Doi:10.1371/journal.pone.0145954.
[14]de Freitas MCD,de Miranda MB,de Oliveira DT,Vieira-Filho SA,Caligiorne RB,de Figueiredo SM.Biological activities of red propolis:Areview.Recent Pat Endocr Metab Immune Drug Discov 2017;11(1):3-12.Doi:10.2174/1872214812666180223120316.
[15]Barbosa Bezerra GB,de Menezes de Souza L,Dos Santos AS,de Almeida GK,Souza MT,Santos SL,et al.Hydroalcoholic extract of Brazilian red propolis exerts protective effects on acetic acid-induced ulcerative colitis in a rodent model.Biomed Pharmacother 2017;85:687-696.Doi:10.1016/j.biopha.2016.11.080.
[16]Paulino N,Dantas AP,Bankova V,Longhi DT,Scremin A,de Castro SL,et al.Bulgarian propolis induces analgesic and anti-inflammatory effects in mice and inhibits in vitro contraction of airway smooth muscle.JPharmacol Sci 2003;93(3):307-313.
[17]Zancanela DC,Funari CS,Herculano RD,Mello VM,Rodrigues CM,Borges FA,et al.Natural rubber latex membranes incorporated with three different types of propolis:Physical-chemistry and antimicrobial behaviours.Mater Sci Eng C Mater Biol Appl 2019;97:576-582.Doi:10.1016/j.msec.2018.12.042.
[18]Iqbal S,Bhanger MI.Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan.J Food Compost Anal 2006;19(6-7):544-551.Doi:10.1016/j.jfca.2005.05.001.
[19]Kasai H,Kubota Y.Analyses of volatile components of lavender(Lavandula angustifolia HIDCOTE and Lavandula xintermedia GROSSO)as influenced by cultivar type,part,and growth season.Yakugaku Zasshi2018;138(12):1569-1577.Doi:10.1248/yakushi.18-00159.
[20]?krovánkováS,Mi?urcováL,Mach?L.Antioxidant activity and protecting health effects of common medicinal plants.Adv Food Nutr Res2012;67:75-139.Doi:10.1016/B978-0-12-394598-3.00003-4.
[21]Morris GP,Beck PL,Herridge MS,Depew WT,Szewczuk MR,Wallace JL.Hapten-induced model of chronic inflammation and ulceration in the rat colon.Gastroenterology 1989;96(3):795-803.
[22]Ku SK,Seo BI,Park JH,Park GY,Seo YB,Kim JS,et al.Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity.World J Gastroenterol 2009;15(38):4799-4805.
[23]Ohkawa H,Ohishi N,Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Anal Biochem 1979;95(2):351-258.
[24]Krawisz JE,Sharon P,Stenson WF.Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity:Assessment of inflammation in rat and hamster models.Gastroenterology 1984;87(6):1344-1350.
[25]Miranda KM,Espey MG,Wink DA.A rapid,simple spectrophotometric method for simultaneous detection of nitrate and nitrite.Nitric Oxide2001;5(1):62-71.
[26]Kulmacz RJ,Lands WE.Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase.Prostaglandins 1983;25(4):531-540.
[27]Sreelatha S,Padma P.Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity.Plant Foods Hum Nutr2009;64(4):303-311.Doi:10.1007/s11130-009-0141-0.
[28]Suvarna KS,Layton C,Bancroft JD.Bancroft’s theory and practice of histological techniques e.Book[Online].7th ed.Churchill Livingstone:Elsevier Health Sciences;2012.Available from:https://books.google.com.eg/books?id=w PPU4Ny Gm3g C&printsec=frontcover&source=g bs_ge_summary_r&cad=0#v=onepage&q&f=false[Accessed on 1 Oct2012].
[29]Mac Pherson BR,Pfeiffer CJ.Experimental production of diffuse colitis in rats.Digestion 1978;17(2):135-150.
[30]Gahring LC,Carlson NG,Kulmer RA,Rogers SW.Neuronal expression of tumor necrosis factor alpha in the OVOUJI fme brain.Neuroimmunomodulation 1996;3(5):289-303.Doi:10.1159/000097283.
[31]Walsh LJ,Trinchieri G,Waldorf HA,Whitaker D,Murphy GF.Human dermal mast cells contain and release tumor necrosis factor alpha,which induces endothelial leukocyte adhesion molecule 1.Proc Natl Acad Sci USA 1991;88(10):4220-4224.
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