骨髓基质干细胞在治疗脊髓损伤中的应用
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摘要
骨髓基质干细胞(BMSCs)具有取材简单、增殖速度快、抗原性小和培养过程中始终保持多向分化的潜能等特点,已经成为干细胞研究领域的热点,是最好的组织工程种子细胞之一。近年研究发现这类细胞具有高度分化的潜能,能分化为神经细胞,为脊髓损伤(SCI)的修复提供了一条新的途径,具有广阔的临床应用前景。
Bone marrow stromal cells (BMSCs) has been considered the hot spot in the study of stem cells owing to their characteristic that is easy to be acquired and be cultured in vitro,negligible antigenicity and keeping multidifferention potentiality and so on.Now,it is one of the best seed cells for tissue engineering stem.In recent years,it is found that marrow stromal cell has well-differentiated potential and it can differentiate into nerve cell and then provide a new strategy to the reparation of spinal cord injury,and it will be using comprehensively in clinical application.
Bone marrow stromal cells (BMSCs) has been considered the hot spot in the study of stem cells owing to their characteristic that is easy to be acquired and be cultured in vitro,negligible antigenicity and keeping multidifferention potentiality and so on.Now,it is one of the best seed cells for tissue engineering stem.In recent years,it is found that marrow stromal cell has well-differentiated potential and it can differentiate into nerve cell and then provide a new strategy to the reparation of spinal cord injury,and it will be using comprehensively in clinical application.
引文
[1]李建军,杨明亮,杨德刚,等.创伤性脊柱脊髓损伤评估、治疗与康复专家共识[J].中国康复理论与实践,2017,23(3):274-287.
[2]王鹏,孙桂森,郭隆升,等.急性脊髓损伤的药物治疗[J].医学与哲学(B),2017,38(6):72-75.
[3]勾丽洁,许世奇,刘旭东,等.早期康复对脊髓损伤患者功能恢复及并发症的影响[J].中国老年学杂志,2014,34(2):313-315.
[4]孙丽,于丽,张华芳,等.人脐带血间充质干细胞的分离培养及生物学特性[J].解剖科学进展,2011,17(2):131-134.
[5]Xiao Y,Wang Y,Li L,et al.Homing of chloromethy lbenzoylammonia-lableled bone marrow mesenchymal stem cells in animmune mediated bone marrow failure mouse model in vivo[J].Genet Mol Res,2014,13(1):11-21.
[6]Forostyak S,Jendelova P,Sykova E.The role of mesenchymal stromal cells in spinal cord injury,regenerative medicine and possible clinical applications[J].Biochimie,2013,95(12):2257-2270.
[7]Tamir A,Petrocelli T,Stetler K,et al.Stem cell factor inhibits erythroid differentiation by modulating the activity of Glcyclin dependent kinase complexes:a role for p27 in erythroid differentiation compled G1 arrest[J].Cell Growth Differ,2000,11(5):269-277.
[8]徐亦辰,刘玲珑,赵文婧,等.骨髓间充质干细胞分化为心肌样细胞相关基因表达与形态结构发生的关联[J].中国组织工程研究,2017,21(13):1974-1979.
[9]Brazerlton T R,Rossi F M,Keshet G I,et al.From marrow to brain:expression of neuronal phenotypers in adult mice[J].Science,2010,290(5497):1775-1779.
[10]周雪颖,李双月,曲淑贤,等.miR-let-7b慢病毒载体促进大鼠骨髓间充质干细胞向神经细胞分化[J].中国细胞生物学学报,2018,40(7):1146-1152.
[11]郑盛,杨涓,唐映梅.间充质干细胞在炎症免疫调节中的作用及应用进展[J].中国组织工程研究,2015,19(45):7362-7368.
[12]唐夏泓,陈锋.骨髓间充质干细胞在心搏骤停后脑复苏中的研究进展[J/OL].创伤与急诊电子杂志,2015,3(4):13-17.
[13]Cho D I,Kim M R,Jeong H Y,et al.Mesenchymal stem cells reciprocally regulate the M1/M2 balance in mouse bone marrow derived macrophages[J].Exp Mol Med,2014,46(1):e70.
[14]Ribeiro A,Laranjeira P,Mendes S,et al.Mesenchymal stem cells from umbilical cord matrix,adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B,natural killer and T cells[J].Stem Cell Res Ther,2013,4(5):125.
[15]李兰兰,李宁,杨晓菲,等.人脐带间充质干细胞分化为胰岛素分泌细胞的免疫原性变化[J].中国组织工程研究,2018,22(13):2045-2050.
[16]郭德镔,朱向情,潘兴华.骨髓间充质干细胞修复辐射造血损伤的机制[J].中国组织工程研究,2017,21(21):3407-3413.
[17]陈梓欣,周政,冼绍祥,等.不同注射容积的骨髓间充质干细胞移植对心梗后心衰大鼠的影响[J].中华中医药杂志,2018,33(7):3061-3065.
[18]Smith J,Plchampally R,Perry A,et al.Isolation of a highly clono genic and multipotential subfraction of adult stem cells from bone marrow stroma[J].Stem Cells,2004,22(5):823-831.
[19]Shen L H,Li J,Chen J,et al.One year follow up after bone marrow stromal cell treatment in middle-aged female rats with stroke[J].Stroke,2007,38(7):2150-2156.
[20]郑盛,杨涓,唐映梅.间充质干细胞在炎症免疫调节中的作用及应用进展[J].中国组织工程研究,2015,19(45):7362-7368.
[21]马彦,臧爱萍,杨焕凤,等.间充质干细胞在治疗移植物抗宿主病中的应用[J].内科理论与实践,2018,13(2):124-128.
[22]Ritfeld G J,Rauck B M,Novosat T L,et al.The effect of a Polyurethane based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair[J].Biomaterials,2014,35(6):1924-1931.
[23]Lu P,Jones L L,Snyder E Y,et al.Neural stem cells constitutively secrete neurotrophic factors and promote extensive hostaxonal growth after spinal cord injury[J].Exp Neurol,2003,181(2):115-129.
[24]孙佳佳,周军,杨惠林.骨髓间充质干细胞移植治疗脊髓损伤的研究进展[J].中华创伤杂志,2015,31(6):568-570.
[25]Zeng X,Qiu X C,Ma Y H,et al.Integration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transection[J].Biomaterials,2015,53:184-201.
[26]Zeng R,Wang L W,Hu Z B,et al.Differentiation of human bone marrow mesenchymal stem cells into neuron-like cells in vitro[J].Spine(Phila Pa 1976),2011,36(13):997-1005.
[27]赵锡武,刘鑫,荣辉,等.神经干细胞和骨髓间充质干细胞移植治疗脊髓损伤效果与机制对比[J].中国实用神经疾病杂志,2016,19(9):15-17.
[28]冯婷婷,叶英,许铁.脑脊液诱导的大鼠BMSC是移植对脊髓损伤大鼠神经营养因子分泌的影响[J].中国急救复苏与灾害医学杂志,2015,10(7):612-615.
[29]Tsumuraya T,Ohtaki H,Song D,et al.Human mesenchymal stem/stromal cells suppress spinal inflammation in mice with contribution of pituitary adenylate cyclase-activating polypeptide(PACAP)[J].J Neuroinflammation,2015,12:35.
[30]杨新明,胡长波,成垚昱.脊髓损伤机制及骨髓间充质干细胞移植治疗机制的研究[J].生物骨科材料与临床研究,2016,13(6):68-71.
[31]Hu J Z,Long H,Wu T D,et al.The effect of estrogen related receptorαon the regulation of angiogenesis after spinal cord injury[J].Neuroscience,2015,290:570-580.
[32]石良晨,秦峰.胶质细胞源性神经营养因子修饰骨髓间充质干细胞移植与传统方法治疗脊髓损伤研究进展[J].中华实用诊断与治疗杂志,2015,29(5):423-425.
[33]Ding Y,Yan Q,Ruan J W,et al.Electroacupuncture promotes the differentiation of transplanted bone marrow mesenchymal stem cells overexpressing Trk C into neuron-like cells in transected spinal cord of rats[J].Cell Transplant,2013,22(1):65-86.
[34]Cantinieaux D,Quertainmont R,Blacher S,et al.Conditioned medium from bone marrow derived mesenchymal stem cells improves recovery after spinal cord injury in rats:an original strategy to avoid cell transplantation[J].Plos One,2013,8(8):e69515.
[35]Ritfeld G J,Nandoe Tewarie R D,Vajn K,et al.Bone marrow stromal cell mediated tissue sparing enhances functional repair after spinal cord contusion in adult rats[J].Cell Transplant,2012,21(7):1561-1575.
[36]Yazdani S O,Hafizi M,Zali A R,et al.Safety and possible outcome assessment of autologous Schwann cell and bone marrow mesenchymal stromal cell co-transplantation for treatment of patients with chronic spinal cord injury[J].Cytotherapy,2013,15(7):782-791.
[37]Pal R,Venkataramana N K,Bansal A,et al.Ex vivo expanded autologous bone marrow derived mesenchymal stromal cells in human spinal cord injury paraplegia a pilot clinical study[J].Cytotherapy,2009,11(7):897-911.
[38]SykováE,Homola A,Mazanec R,et al.Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury[J].Cell Transplant,2006,15(8-9):675-687.
[39]Moviglia G,Fernandez V R,Brizuela J,et al.Combined protocol of cell therapy for chronic spinal cord injury:report on the electrical and functional recovery of two patients[J].Cytotherapy,2006,8(3):202-209.
[40]Saito F,Nakatani T,Iwase M,et al.Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation:the first clinical trial case report[J].J Trauma,2008,64(1):53-59.
[41]赵廷宝,卢兆桐,赵凌云,等.骨髓间充质干细胞与嗅鞘细胞联合移植治疗脊髓损伤的早期观察[J].中国矫形外科杂志,2006,39(3):346-348.
[42]Bhanot Y,Rao S,Ghosh D,et al.Autologous mesenchymal stem cells in chronic spinal cord injury[J].Br J Neurosurg,2011,25(4):516-522.
[2]王鹏,孙桂森,郭隆升,等.急性脊髓损伤的药物治疗[J].医学与哲学(B),2017,38(6):72-75.
[3]勾丽洁,许世奇,刘旭东,等.早期康复对脊髓损伤患者功能恢复及并发症的影响[J].中国老年学杂志,2014,34(2):313-315.
[4]孙丽,于丽,张华芳,等.人脐带血间充质干细胞的分离培养及生物学特性[J].解剖科学进展,2011,17(2):131-134.
[5]Xiao Y,Wang Y,Li L,et al.Homing of chloromethy lbenzoylammonia-lableled bone marrow mesenchymal stem cells in animmune mediated bone marrow failure mouse model in vivo[J].Genet Mol Res,2014,13(1):11-21.
[6]Forostyak S,Jendelova P,Sykova E.The role of mesenchymal stromal cells in spinal cord injury,regenerative medicine and possible clinical applications[J].Biochimie,2013,95(12):2257-2270.
[7]Tamir A,Petrocelli T,Stetler K,et al.Stem cell factor inhibits erythroid differentiation by modulating the activity of Glcyclin dependent kinase complexes:a role for p27 in erythroid differentiation compled G1 arrest[J].Cell Growth Differ,2000,11(5):269-277.
[8]徐亦辰,刘玲珑,赵文婧,等.骨髓间充质干细胞分化为心肌样细胞相关基因表达与形态结构发生的关联[J].中国组织工程研究,2017,21(13):1974-1979.
[9]Brazerlton T R,Rossi F M,Keshet G I,et al.From marrow to brain:expression of neuronal phenotypers in adult mice[J].Science,2010,290(5497):1775-1779.
[10]周雪颖,李双月,曲淑贤,等.miR-let-7b慢病毒载体促进大鼠骨髓间充质干细胞向神经细胞分化[J].中国细胞生物学学报,2018,40(7):1146-1152.
[11]郑盛,杨涓,唐映梅.间充质干细胞在炎症免疫调节中的作用及应用进展[J].中国组织工程研究,2015,19(45):7362-7368.
[12]唐夏泓,陈锋.骨髓间充质干细胞在心搏骤停后脑复苏中的研究进展[J/OL].创伤与急诊电子杂志,2015,3(4):13-17.
[13]Cho D I,Kim M R,Jeong H Y,et al.Mesenchymal stem cells reciprocally regulate the M1/M2 balance in mouse bone marrow derived macrophages[J].Exp Mol Med,2014,46(1):e70.
[14]Ribeiro A,Laranjeira P,Mendes S,et al.Mesenchymal stem cells from umbilical cord matrix,adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B,natural killer and T cells[J].Stem Cell Res Ther,2013,4(5):125.
[15]李兰兰,李宁,杨晓菲,等.人脐带间充质干细胞分化为胰岛素分泌细胞的免疫原性变化[J].中国组织工程研究,2018,22(13):2045-2050.
[16]郭德镔,朱向情,潘兴华.骨髓间充质干细胞修复辐射造血损伤的机制[J].中国组织工程研究,2017,21(21):3407-3413.
[17]陈梓欣,周政,冼绍祥,等.不同注射容积的骨髓间充质干细胞移植对心梗后心衰大鼠的影响[J].中华中医药杂志,2018,33(7):3061-3065.
[18]Smith J,Plchampally R,Perry A,et al.Isolation of a highly clono genic and multipotential subfraction of adult stem cells from bone marrow stroma[J].Stem Cells,2004,22(5):823-831.
[19]Shen L H,Li J,Chen J,et al.One year follow up after bone marrow stromal cell treatment in middle-aged female rats with stroke[J].Stroke,2007,38(7):2150-2156.
[20]郑盛,杨涓,唐映梅.间充质干细胞在炎症免疫调节中的作用及应用进展[J].中国组织工程研究,2015,19(45):7362-7368.
[21]马彦,臧爱萍,杨焕凤,等.间充质干细胞在治疗移植物抗宿主病中的应用[J].内科理论与实践,2018,13(2):124-128.
[22]Ritfeld G J,Rauck B M,Novosat T L,et al.The effect of a Polyurethane based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair[J].Biomaterials,2014,35(6):1924-1931.
[23]Lu P,Jones L L,Snyder E Y,et al.Neural stem cells constitutively secrete neurotrophic factors and promote extensive hostaxonal growth after spinal cord injury[J].Exp Neurol,2003,181(2):115-129.
[24]孙佳佳,周军,杨惠林.骨髓间充质干细胞移植治疗脊髓损伤的研究进展[J].中华创伤杂志,2015,31(6):568-570.
[25]Zeng X,Qiu X C,Ma Y H,et al.Integration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transection[J].Biomaterials,2015,53:184-201.
[26]Zeng R,Wang L W,Hu Z B,et al.Differentiation of human bone marrow mesenchymal stem cells into neuron-like cells in vitro[J].Spine(Phila Pa 1976),2011,36(13):997-1005.
[27]赵锡武,刘鑫,荣辉,等.神经干细胞和骨髓间充质干细胞移植治疗脊髓损伤效果与机制对比[J].中国实用神经疾病杂志,2016,19(9):15-17.
[28]冯婷婷,叶英,许铁.脑脊液诱导的大鼠BMSC是移植对脊髓损伤大鼠神经营养因子分泌的影响[J].中国急救复苏与灾害医学杂志,2015,10(7):612-615.
[29]Tsumuraya T,Ohtaki H,Song D,et al.Human mesenchymal stem/stromal cells suppress spinal inflammation in mice with contribution of pituitary adenylate cyclase-activating polypeptide(PACAP)[J].J Neuroinflammation,2015,12:35.
[30]杨新明,胡长波,成垚昱.脊髓损伤机制及骨髓间充质干细胞移植治疗机制的研究[J].生物骨科材料与临床研究,2016,13(6):68-71.
[31]Hu J Z,Long H,Wu T D,et al.The effect of estrogen related receptorαon the regulation of angiogenesis after spinal cord injury[J].Neuroscience,2015,290:570-580.
[32]石良晨,秦峰.胶质细胞源性神经营养因子修饰骨髓间充质干细胞移植与传统方法治疗脊髓损伤研究进展[J].中华实用诊断与治疗杂志,2015,29(5):423-425.
[33]Ding Y,Yan Q,Ruan J W,et al.Electroacupuncture promotes the differentiation of transplanted bone marrow mesenchymal stem cells overexpressing Trk C into neuron-like cells in transected spinal cord of rats[J].Cell Transplant,2013,22(1):65-86.
[34]Cantinieaux D,Quertainmont R,Blacher S,et al.Conditioned medium from bone marrow derived mesenchymal stem cells improves recovery after spinal cord injury in rats:an original strategy to avoid cell transplantation[J].Plos One,2013,8(8):e69515.
[35]Ritfeld G J,Nandoe Tewarie R D,Vajn K,et al.Bone marrow stromal cell mediated tissue sparing enhances functional repair after spinal cord contusion in adult rats[J].Cell Transplant,2012,21(7):1561-1575.
[36]Yazdani S O,Hafizi M,Zali A R,et al.Safety and possible outcome assessment of autologous Schwann cell and bone marrow mesenchymal stromal cell co-transplantation for treatment of patients with chronic spinal cord injury[J].Cytotherapy,2013,15(7):782-791.
[37]Pal R,Venkataramana N K,Bansal A,et al.Ex vivo expanded autologous bone marrow derived mesenchymal stromal cells in human spinal cord injury paraplegia a pilot clinical study[J].Cytotherapy,2009,11(7):897-911.
[38]SykováE,Homola A,Mazanec R,et al.Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury[J].Cell Transplant,2006,15(8-9):675-687.
[39]Moviglia G,Fernandez V R,Brizuela J,et al.Combined protocol of cell therapy for chronic spinal cord injury:report on the electrical and functional recovery of two patients[J].Cytotherapy,2006,8(3):202-209.
[40]Saito F,Nakatani T,Iwase M,et al.Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation:the first clinical trial case report[J].J Trauma,2008,64(1):53-59.
[41]赵廷宝,卢兆桐,赵凌云,等.骨髓间充质干细胞与嗅鞘细胞联合移植治疗脊髓损伤的早期观察[J].中国矫形外科杂志,2006,39(3):346-348.
[42]Bhanot Y,Rao S,Ghosh D,et al.Autologous mesenchymal stem cells in chronic spinal cord injury[J].Br J Neurosurg,2011,25(4):516-522.