云南甜龙竹组培及组培苗移栽技术研究
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摘要
选择6种类型的云南甜龙竹外植体,经不同浓度的升汞溶液消毒一定时间,在添加不同浓度6-BA的MS培养基中进行组培试验,以筛选云南甜龙竹组培繁殖的适宜条件;同时,以1年生云南甜龙竹组培苗为材料,在6种育苗基质和4种育苗环境下试验组培苗的生长情况,以筛选适宜组培苗生长的最佳条件。结果表明:云南甜龙竹组培繁育以1年生种子苗茎段作为外植体,用0.10%～0.15%的升汞消毒6～9 min,使用标准MS培养基,添加浓度为1.5～2.0 g/mL的6-BA,能够获得较高的芽诱导率;1年生组培苗移栽时,采用大棚套小棚的移栽环境,以植物碎沫作为栽培基质,苗木成活率较高,幼苗生长较好。
Six types of explants were selected from Dendrocalamus brandiss and after disinfected with different concentrations of mercuric chloride solution for a certain period of time, they were cultivated in MS medium supplemented with different concentrations of 6-BA, in order to screen the appropriate conditions for tissue culture of D. brandiss. Meanwhile, 1-year-old tissue culture seedlings were tested under 6 kinds of seedling substrates and 4 different environmental conditions to screen the optimal conditions suitable for the growth of tissue culture seedlings. The results showed that the explants of young culm from 1-year-old seedlings which were disinfected with 0.10%-0.15% mercuric chloride for 6-9 min and cultivated in standard MS medium with 1.5-2.0 g/mL of 6-BA, could achieve higher bud induction rate. When the 1-year-old tissue culture seedlings were transplanted, the seedlings were covered in greenhouse and cultivated with the fine particles made from plant debris, which could achieved higher survival rate and better growth.
Six types of explants were selected from Dendrocalamus brandiss and after disinfected with different concentrations of mercuric chloride solution for a certain period of time, they were cultivated in MS medium supplemented with different concentrations of 6-BA, in order to screen the appropriate conditions for tissue culture of D. brandiss. Meanwhile, 1-year-old tissue culture seedlings were tested under 6 kinds of seedling substrates and 4 different environmental conditions to screen the optimal conditions suitable for the growth of tissue culture seedlings. The results showed that the explants of young culm from 1-year-old seedlings which were disinfected with 0.10%-0.15% mercuric chloride for 6-9 min and cultivated in standard MS medium with 1.5-2.0 g/mL of 6-BA, could achieve higher bud induction rate. When the 1-year-old tissue culture seedlings were transplanted, the seedlings were covered in greenhouse and cultivated with the fine particles made from plant debris, which could achieved higher survival rate and better growth.
引文
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[3] Alexander,M P,Ramana Rao T C.In vitro culture of bamboo embryos[J].Current Science,1968,37:415.
[4] Sood A,Ahuja P S,Sharma M,et al.In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn.Ex Munro[J].Plant Cell Tissue and Organ Culture,2002,71(1):55-63.
[5] Godbole S,Sood A,Thakur R,et al.Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo,Dendrocalamus hamiltonii Nees et Arn.Ex Munro[J].Current Science,2002(83):885-889.
[6] 江泽慧.世界竹藤[M].沈阳:辽宁科学技术出版社,2002.
[7] 杨汉奇,孙茂盛,阮桢媛,等.云南4种典型热带丛生竹的种源分化[J].林业科学研究,2014,27(2):168-173.
[8] 张宁.版纳甜龙竹愈伤组织诱导与植株再生体系的建立[D].浙江临安:浙江农林大学,2011.
[9] 苏海,钟明,蔡时可,等.马来甜龙竹的组织培养和快速繁殖[J].植物生理学通讯,2004(4):468.