应用micro-CT检测复合树脂充填体微渗漏面积的研究
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摘要
目的:应用显微CT(micro-CT)观测比较Ⅴ类洞4种复合树脂充填体的边缘微渗漏情况。方法:选取40颗人离体磨牙制备Ⅴ类洞,随机分为4组(n=10),分别用同一种粘接剂及4种光固化复合树脂充填修复:A组(3M Filtek Z350 XT通用型)、B组(Clearfil AP-X)、C组(Dentsply Spectrum TPH)、D组(3M Filtek P60)。再将试样浸入氨化硝酸银溶液12 h及显影液中8 h。经micro-CT扫描试样后,重建洞壁银(Ag)渗漏三维图像,测定充填体洞壁的整体银渗漏面积。采用单因素方差分析比较4种树脂充填体的微渗漏面积差异。结果:充填体微渗漏面积A组与B组差异无统计学意义(P>0.05);D组渗漏面积最大,显著大于其余3组(P<0.01);C组渗漏面积显著大于A组和B组(P<0.01)。结论:Micro-CT能建立银渗漏三维形态并精确测定窝洞渗漏面积;3M Filtek P60后牙充填体的微渗漏较其他三种通用型复合树脂充填体微渗漏严重。
Objective:To observe and compare the marginal microleakage of Class V restorations of 4 kinds of resin composites by using micro-CT.Methods:Class V cavity preparations were made in 40 extracted non-carious human molars,which were randomly divided into 4 groups(n=10).They were restored with the same dental adhesive and 4 kinds of resin composites respectively,that group A(3 M Filtek Z350 XT Universal Restorative),group B(Clearfil AP-X),group C(Dentsply Spectrum TPH)and group D(3 M Filtek P60).Afterward,all teeth were then immersed in 50% ammoniacal silver nitrate solution for 12 hours,followed by a developing solution for 8 hours.At last,each tooth was scanned and the three-dimensional image of the silver leakage around cavity wall were reconstructed by micro-CT,and then the area of marginal silver leakage was measured.The single factor variance analysis was used to compare the leakage areas of four resin restorations.Results:There was no significant difference between group A and B(P>0.05).Group D has the largest leakage area,and is significantly larger than the other three groups(P<0.01),and group C leakage area was significantly larger than group A and B respectively(P<0.01).Conclusion:Micro-CT can display the three-dimensional shape of mircoleakage fully and detect the silver leakage area of restoration accurately.The microleakage of the 3 M Filtek P60 restoration is more serious than that of the three universal resin composite restorations.
Objective:To observe and compare the marginal microleakage of Class V restorations of 4 kinds of resin composites by using micro-CT.Methods:Class V cavity preparations were made in 40 extracted non-carious human molars,which were randomly divided into 4 groups(n=10).They were restored with the same dental adhesive and 4 kinds of resin composites respectively,that group A(3 M Filtek Z350 XT Universal Restorative),group B(Clearfil AP-X),group C(Dentsply Spectrum TPH)and group D(3 M Filtek P60).Afterward,all teeth were then immersed in 50% ammoniacal silver nitrate solution for 12 hours,followed by a developing solution for 8 hours.At last,each tooth was scanned and the three-dimensional image of the silver leakage around cavity wall were reconstructed by micro-CT,and then the area of marginal silver leakage was measured.The single factor variance analysis was used to compare the leakage areas of four resin restorations.Results:There was no significant difference between group A and B(P>0.05).Group D has the largest leakage area,and is significantly larger than the other three groups(P<0.01),and group C leakage area was significantly larger than group A and B respectively(P<0.01).Conclusion:Micro-CT can display the three-dimensional shape of mircoleakage fully and detect the silver leakage area of restoration accurately.The microleakage of the 3 M Filtek P60 restoration is more serious than that of the three universal resin composite restorations.
引文
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[13]Condon JR,Ferracane JL.Reduction of composite contraction stress through non-bonded microfiller particles[J].Dent Mater,1998,14(4):256-260.
[2]古林娟,赵信义,李石保.应用显微CT观测Ⅴ类洞修复体边缘渗漏初探[J].中华口腔医学杂志,2012,47(9):532-537.
[3]Zhao XY,Li SB,Gu LJ,et al.Detection of marginal leakage of class Vrestorations in vitro by micro-computed tomography[J].Oper Dent,2014,39(2):174-180.
[4]Kaisarly D,Gezawi ME.Polymerization shrinkage assessment of dental resin composites:a literature review[J].Odontology,2016,104(3):257-270.
[5]赵岩,高平.复合树脂充填体边缘微渗漏的微型CT检测研究[J].实用口腔医学杂志,2013,29(6):791-794.
[6]Iwami Y,Shimizu A,Hayashi M,et al.Three-dimensional evaluation of gap formation of cervical restorations[J].J Dent,2005,33(4):325-333.
[7]Sun J,Eidelman N,Lin-Gibson S.3D mapping of polymerization shrinkage using X-ray micro-computed tomography to predict microleakage[J].Dent Mater,2009,25(3):314-320.
[8]Rengo C,Goracci C,Ametrano G,et al.Marginal leakage of class Vcomposite restorations assessed using micro computed tomography and scanning electron microscope[J].Oper Dent,2015,40(4):440-448.
[9]Eden E,Topaloglu-Ak A,Cuijpers V,et a1.Micro-CT for measuring marginal leakage of Class II resin composite restorations in primary molars prepared in vivo[J].Am J Dent,2008,21(6):393-397.
[10]De Santis R,Mollica F,Prisco D,et a1.A 3D analysis of mechanically stressed dentin-adhesive-composite interfaces using X-ray micro-CT[J].Biomaterials,2005,26,(3):257-270.
[11]Carrera CA,Lan C,Escobar-Sanabria D,et a1.The use of micro-CTwith image segmentation to quantify leakage in dental restorations[J].Dent Mater,2015,31(4):382-390.
[12]王新迎.不同粘结剂与不同复合树脂修复V类洞的微渗漏影响[D].乌鲁木齐:新疆医科大学.2017.
[13]Condon JR,Ferracane JL.Reduction of composite contraction stress through non-bonded microfiller particles[J].Dent Mater,1998,14(4):256-260.