硝酸镧浸种对低磷胁迫下红小豆幼苗生长的影响
|
摘要
采用1/2 Hongland缺磷营养液模拟低磷胁迫的方式,利用水培法研究了在低磷胁迫下,不同浓度硝酸镧(0,20,50,100 mg·L~(-1))浸种对2个品种(保红8824-17和白红4号)红小豆幼苗地上部分生长的影响。结果表明:与对照组相比,硝酸镧浸种增加了2个品种红小豆幼苗的株高、叶面积、鲜重、干重和根冠比、降低了茎粗,但2个品种随硝酸镧浓度增加表现的趋势不一致;随着硝酸镧浓度增加,保红8824-17的POD活性呈先升高后降低的趋势、SOD活性表现出先降低后升高的趋势,白红4号的SOD活性的变化趋势为下降,POD活性的变化趋势为上升;2个品种MDA含量都表现为随浓度的增加而先降低后升高,当浸种浓度为50 mg·L~(-1)时达到最低值;随着浸种时硝酸镧浓度的增加,保红8824-17的叶绿素含量的变化趋势为先增大后减小,白红4号的叶绿素含量持续增大。综上所述,用硝酸镧对红小豆进行浸种可缓解低磷胁迫对红小豆幼苗生长产生的影响,最适浸种浓度为50 mg·L~(-1)。
The effect of seed soaking with different concentrations of lanthanum nitrate(0,20,50,100 mg·L~(-1))on seedling growth of 2 varieties red akuki(red preserving 8824-17 and white-red No.4) under low phosphorus stress was studied by hydroponics and 1/2 hongland phosphorus deficient nutrient fluid to simulate low phosphorus stress method.The results showed that compared with the control group,lanthanum nitrate soaking increased the plant height,leaf area,fresh weight,dry weight and root crown ratio of the two varieties,and reduced the stem diameter,but the two varieties showed different trend with the increase of lanthanum nitrate concentration.With the increase of lanthanum nitrate concentration, the POD activity of red preserving 8824-17 showed a trend of first increase and then decrease,and the SOD activity showed a trend of first decrease and then increase.The variation trend of SOD activity of white-red No.4 was decreased, and that of POD activity was increased. MDA content of both varieties decreased first and then increased with the increase of concentration,reaching the lowest value when the soaking concentration reached 50 mg·L~(-1).With the increase of lanthanum nitrate concentration during seed soaking,the variation trend of chlorophyll content of red preserving 8824-17 first increased and then decreased,and the chlorophyll content of white-red No.4 continued to increase. In summary,soaking seeds with lanthanum nitrate can alleviate the effects of low phosphorus stress on the growth of red adzuki bean seedlings,and the optimum soaking concentration was 50 mg·L~(-1).
The effect of seed soaking with different concentrations of lanthanum nitrate(0,20,50,100 mg·L~(-1))on seedling growth of 2 varieties red akuki(red preserving 8824-17 and white-red No.4) under low phosphorus stress was studied by hydroponics and 1/2 hongland phosphorus deficient nutrient fluid to simulate low phosphorus stress method.The results showed that compared with the control group,lanthanum nitrate soaking increased the plant height,leaf area,fresh weight,dry weight and root crown ratio of the two varieties,and reduced the stem diameter,but the two varieties showed different trend with the increase of lanthanum nitrate concentration.With the increase of lanthanum nitrate concentration, the POD activity of red preserving 8824-17 showed a trend of first increase and then decrease,and the SOD activity showed a trend of first decrease and then increase.The variation trend of SOD activity of white-red No.4 was decreased, and that of POD activity was increased. MDA content of both varieties decreased first and then increased with the increase of concentration,reaching the lowest value when the soaking concentration reached 50 mg·L~(-1).With the increase of lanthanum nitrate concentration during seed soaking,the variation trend of chlorophyll content of red preserving 8824-17 first increased and then decreased,and the chlorophyll content of white-red No.4 continued to increase. In summary,soaking seeds with lanthanum nitrate can alleviate the effects of low phosphorus stress on the growth of red adzuki bean seedlings,and the optimum soaking concentration was 50 mg·L~(-1).
引文
[1]范曾丽,王三根.不同基因型玉米自交系在低磷胁迫下的生理生化特性比较初探[J].西华师范大学学报(自然科学版),2005,26(3):257-261.
[2]丁艳,杜庆才,孙兰萍,等.Cr,Pb单一及复合污染对小麦叶绿素含量的影响[J].重庆文理学院学报(自然科学版),2013,31(6):49-52.
[3]张永清,刘凤兰,贾蕊,等.La (NO3)3浸种对盐碱胁迫下红小豆幼苗生长和抗氧化酶活性的影响[J].生态与农村环境学报,2009,25(4):12-18.
[4]何跃君,薛立.稀土元素对植物的生物效应及其作用机理[J].应用生态学报,2005,16(10):1983-1989.
[5]解惠光.中国稀土元素在农业上应用研究进展[J].科学通报,1991,36(8):561-564.
[6]刘凤兰,张永清,贾蕊,等.La(NO3)3浸种对NaCl胁迫下红小豆幼苗生长的影响[J].武汉植物学研究,2009(4):397-402.
[7]王静,安忠民,冯学赞.硝酸镧对马铃薯试管苗生长的影响[J].植物生理学通讯,2003,39(1):25-26.
[8]王爱国,罗广华,邵从本,等.大豆种子超氧物歧化酶的研究[J].植物生理学报,1983,9(1):77-84.
[9]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:68-77.
[10]曹翠玲,毛圆辉,曹朋涛,等.低磷胁迫对豇豆幼苗叶片光合特性及根系生理特性的影响[J].植物营养与肥料学报,2010,16(6):1373-1378.
[11]刘建新,王鑫,胡浩斌,等.硝酸镧对碱胁迫下黑麦草幼苗生长和光合生理的影响[J].植物研究,2010,30(6):674-679.
[12]赵映琴,刘玉汇,王丽,等.低磷胁迫下马铃薯试管苗生长及生理指标变化研究[J].干旱地区农业研究,2009(5):183-187.
[13]高彬,曹翠玲,李涛.乙烯对低磷胁迫下大豆根形态和生理特性的影响[J].大豆科学,2012,31(1):58-63.
[14]陈永亮,李修岭,周晓燕.低磷胁迫对落叶松幼苗生长及根系酸性磷酸酶活性的影响[J].北京林业大学学报,2007,28(6):46-50.
[15]李永夫,金松恒,叶正钱,等.低磷胁迫对山核桃幼苗根系形态和生理特征的影响[J].浙江林学院学报,2010,27(2):239-245.
[16]冉景盛,陈今朝,方平,等.硝酸镧浸种对水稻种子萌发及生理生化特性的影响[J].湖北农业科学,2009,48(2):283-285.
[2]丁艳,杜庆才,孙兰萍,等.Cr,Pb单一及复合污染对小麦叶绿素含量的影响[J].重庆文理学院学报(自然科学版),2013,31(6):49-52.
[3]张永清,刘凤兰,贾蕊,等.La (NO3)3浸种对盐碱胁迫下红小豆幼苗生长和抗氧化酶活性的影响[J].生态与农村环境学报,2009,25(4):12-18.
[4]何跃君,薛立.稀土元素对植物的生物效应及其作用机理[J].应用生态学报,2005,16(10):1983-1989.
[5]解惠光.中国稀土元素在农业上应用研究进展[J].科学通报,1991,36(8):561-564.
[6]刘凤兰,张永清,贾蕊,等.La(NO3)3浸种对NaCl胁迫下红小豆幼苗生长的影响[J].武汉植物学研究,2009(4):397-402.
[7]王静,安忠民,冯学赞.硝酸镧对马铃薯试管苗生长的影响[J].植物生理学通讯,2003,39(1):25-26.
[8]王爱国,罗广华,邵从本,等.大豆种子超氧物歧化酶的研究[J].植物生理学报,1983,9(1):77-84.
[9]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:68-77.
[10]曹翠玲,毛圆辉,曹朋涛,等.低磷胁迫对豇豆幼苗叶片光合特性及根系生理特性的影响[J].植物营养与肥料学报,2010,16(6):1373-1378.
[11]刘建新,王鑫,胡浩斌,等.硝酸镧对碱胁迫下黑麦草幼苗生长和光合生理的影响[J].植物研究,2010,30(6):674-679.
[12]赵映琴,刘玉汇,王丽,等.低磷胁迫下马铃薯试管苗生长及生理指标变化研究[J].干旱地区农业研究,2009(5):183-187.
[13]高彬,曹翠玲,李涛.乙烯对低磷胁迫下大豆根形态和生理特性的影响[J].大豆科学,2012,31(1):58-63.
[14]陈永亮,李修岭,周晓燕.低磷胁迫对落叶松幼苗生长及根系酸性磷酸酶活性的影响[J].北京林业大学学报,2007,28(6):46-50.
[15]李永夫,金松恒,叶正钱,等.低磷胁迫对山核桃幼苗根系形态和生理特征的影响[J].浙江林学院学报,2010,27(2):239-245.
[16]冉景盛,陈今朝,方平,等.硝酸镧浸种对水稻种子萌发及生理生化特性的影响[J].湖北农业科学,2009,48(2):283-285.