干旱诱导的海藻糖和脱落酸对苹果品质的影响
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摘要
为了研究在适度干旱条件下,苹果果实中海藻糖和脱落酸对果实品质提升所起的生理作用。以7～8a生的盆栽嘎啦Gala/M26苹果树为试材,采用高效液相色谱法和酶学测定法,测定不同程度干旱胁迫(田间最大持水量的75%～80%、60%～65%、50%～55%)和果心注射脱落酸(ABA)[0(蒸馏水)和1mmol/L]的果实的果糖、葡萄糖、蔗糖、山梨醇和海藻糖质量分数,内源ABA质量分数,海藻糖代谢相关酶[6-磷酸海藻糖合成酶(TPS)、6-磷酸海藻糖磷酸酯酶(TPP)和海藻糖酶(TRE)]活性。结果显示,干旱处理和ABA处理均使果实内源ABA质量分数增加,有利于果实内糖分的积累,促进TPS和TPP活性,但抑制海藻糖酶的活性。适度干旱条件下所产生的ABA影响海藻糖代谢途径的改变,促进海藻糖的积累,有利于果实中糖分的积累,提升果实品质。
To study the effect of trehalose metabolism pathway and abscisic acid on improvement infruit quality under moderate drought conditions.With 7-8 apotted Gala/M26 apple trees asmaterials,high performance liquid chromatography(HPLC)method and enzyme assay were used to test the different degrees of drought stress(water holding capacity 75%-80%,60%-65%,50%-55%in the field)the mass fraction of the fruits glucose,fructose,sucrose,sorbitol and trehalose under condition of injection of exogenous abscisic acid[0 mmol/L(distilled water)and 1 mmol/L],endogenous ABA mass fraction and activities of trehalose metabolism related to enzyme,such as trehalose-6-phosphate synthase(TPS),trehalose-6-phosphate phosphatase(TPP)and trehalase(TRE)during fruit maturity.The results indicated that both drought treatment and ABA treatment increased the mass fraction of ABA mass fraction in fruits,which was beneficial to accumulate sugar in fruits,and promote TPS and TPP activity,but inhibite the activity of trehalase.In conclusion,ABA affects the metabolic pathway of trehalose in the drought condition,and promotes the accumulation of trehalose,which is beneficial to the accumulation of sugar in the fruit and to increase the fruit quality.
To study the effect of trehalose metabolism pathway and abscisic acid on improvement infruit quality under moderate drought conditions.With 7-8 apotted Gala/M26 apple trees asmaterials,high performance liquid chromatography(HPLC)method and enzyme assay were used to test the different degrees of drought stress(water holding capacity 75%-80%,60%-65%,50%-55%in the field)the mass fraction of the fruits glucose,fructose,sucrose,sorbitol and trehalose under condition of injection of exogenous abscisic acid[0 mmol/L(distilled water)and 1 mmol/L],endogenous ABA mass fraction and activities of trehalose metabolism related to enzyme,such as trehalose-6-phosphate synthase(TPS),trehalose-6-phosphate phosphatase(TPP)and trehalase(TRE)during fruit maturity.The results indicated that both drought treatment and ABA treatment increased the mass fraction of ABA mass fraction in fruits,which was beneficial to accumulate sugar in fruits,and promote TPS and TPP activity,but inhibite the activity of trehalase.In conclusion,ABA affects the metabolic pathway of trehalose in the drought condition,and promotes the accumulation of trehalose,which is beneficial to the accumulation of sugar in the fruit and to increase the fruit quality.
引文
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[12]SMEEKENS S.From leaf to kernel:trehalose-6-phosphate signaling moves carbon in the field[J].Plant Physiology,2015,169(2):912-913.
[13]ALI Z,SARWAT S S,KARIM I,et al.Functions of plant's bZIP transcription factors[J].Pakistan Journal of Agricultural Sciences,2016,53(2),303-314.
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[15]梁俊,郭燕,刘玉莲,等.不同品种苹果果实中糖酸组成与含量分析[J].西北农林科技大学学报(自然科学版),2011,39(10):163-170.LIANG J,GUO Y,LIU Y L,et al.Analysis of contents and constituents of sugar and organic acid in different apple cultivars[J].Journal of Northwest A&F University(Natural Science Edition),2011,39(10):163-170.
[16]GARG N,SINGLA P.Stimulation of nitrogen fixation and trehalose biosynthesis by naringenin(Nar)and arbuscular mycorrhiza(AM)in chickpea under salinity stress[J].Plant Growth Regulation,2016,80(2):5-22.
[17]ILHAN S,OZDEMIR F,BOR M.Contribution of trehalose biosynthetic pathway to drought stress tolerance of Capparis ovata Desf[J].Plant Biology,2015,17(2):402-407.
[18]LOPEZ M,TEJERA N A,IRIBARNE C,et al.Trehalose and trehalase in root nodules of Medicagotruncatulaand Phaseolus vulgaris in response to salt stress[J].Physiologia Plantarum,2008,134(4):575-582.
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[20]谷继成,王有年,姚允聪.苹果幼树对干旱信号ABA响应机理的研究进展[J].北京农学院学报,1996,11(2):90-109.GU J CH,WANG Y N,YAO Y C.Research progress on response mechanism of apple young tree to drought signal ABA[J].Journal of Beijing University of Agricultural,1996,11(2):90-109.
[21]LIU Y M,ZHANG X Z,TRAN H,et al.Assessment of drought tolerance of 49switchgrass(Panicum virgatum)genotypes using physiological and morphological parameters[J].Biotechnology for Biofuels,2015,8:148-152.
[22]ITURRIAGA G,SUAREZ R,NOVA-FRANCO B.Trehalose metabolism:from osmopmtection to signaling[J].International Journal of Molecular Sciences,2009,10:3793-3810.
[23]NAYYAR H,BAINS T,KUMAR S.Low temperature induced floral abortion in chickpea:relationship to abscisic acid and cryoprotectants in reproductive organs[J].Environmental and Experimental Botany,2005,53(1):39-47.
[24]陈素丽,彭瑜,周华,等.植物海藻糖代谢及海藻糖-6-磷酸信号研究进展[J].植物生理学报,2014,50(3):233-242.CHEN S L,PENG Y,ZHOU H,et al.Research advances in trehalose metabolism and trehalose-6-phosphate signaling in plants[J].Plant Physiology Journal,2014,50(3):233-242.
[25]TIAN C J,ZUO Z L,QIU J L.Identification and characterization of ABA-responsive microRNAs in rice[J].Journal of Genetics and Genomics,2015,42(7):393-402.
[26]MULLER J,BOLLET T,WIEMKEN A.Trehalose affects sucrose synthase and invertase activities in soybean(Glycine max L.Merr.)roots[J].Journal of Plant Physiology,1998,153(1/2):255-257.
[27]BARRAZA A,ESTRADA-NAVARRETE G,RODRIGU-EZ-ALEGRIA M E,et al.Down-regulation of PvTRE1enhances nodule biomass and bacteroid number in the common bean[J].New Phytologist,2013,197(1):194-206.
[28]YADAV UP,IVAKOV A,FEIL R,et al.The sucrose-trehalose 6-phosphate(Tre6P)nexus:specificity and mechanisms of sucrose signalling by Tre6P[J].Journal of Experimental Botany,2014,65(4):1051-1068.
[2]魏建梅,齐秀东,朱向秋,等.苹果果实糖积累特性与品质形成的关系[J].西北植物学报,2009,29(6):1193-1199.WEI J M,QI X D,ZHU X Q,et al.Relationship between the characteristics of sugar accumulation and fruit quality in apple(Malus domestica Borkh.)fruit[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(6):1193-1199.
[3]LI F,LEI H J,ZHAO X J.Characterization of three sorbitol transporter genes in micropropagated apple plants grown under drought stress[J].Plant Molecular Biology Reporter,2012,30(1):123-130.
[4]KUHN C,BARKERr L,BURKLE L,FROMMER WB.Update on sucrose transport in higher plants[J].Journal of Experimental Botany,1999,50(Si):935-953.
[5]PAIVAI C L,PANEK A D.Biotechnological applications of the disaccharide trehalose[J].Biotechnology Annual Review,1996,2:293-314.
[6]EASTMOND P J,GRAHAM I A.Trehalose metabolism:a regulatory role for trehalose-6-phosphate[J].Current Opinionin Plant Biology,2003,6(3):231-235.
[7]HARTHII J E,MEEK S M,MORMCEL N,et al.Phosphorylation and 14-3-3binding of Arabidopsis trehalose-phosphate svnthase 5in response to 2-deoxyglucoseI[J].Plant Journal,2006,47(2):211-223.
[8]SCHLUEPMANN H,VAN D A,AGHDASI M,et al.Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation[J].Plant Physiology,2004,135(2):879-890.
[9]BAENA-GONZALEZ E,ROLLAND F,THEVELEIN JM,et al.A central integrator of transcription networks in plant stress and energy signaling[J].Nature,2007,448(7156):938-942.
[10]BAENA-GONZALEZ E,SHEEN J.Convergent energy and stress signaling[J].Trends in Plant Science,2008,13(9):474-482.
[11]NUNES C,OHARA L E,PRIMAVESI L F,et al.The trehalose 6-phosphate/SnRK1signaling pathway primes growth recovery following relief of sink limitation[J].Plant Physiology,2013,162(3):1720-1732.
[12]SMEEKENS S.From leaf to kernel:trehalose-6-phosphate signaling moves carbon in the field[J].Plant Physiology,2015,169(2):912-913.
[13]ALI Z,SARWAT S S,KARIM I,et al.Functions of plant's bZIP transcription factors[J].Pakistan Journal of Agricultural Sciences,2016,53(2),303-314.
[14]LUMBA S,TOH S,HANDFIELD LF,et al.A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis[J].Developmental Cell,2014,29(3):360-372.
[15]梁俊,郭燕,刘玉莲,等.不同品种苹果果实中糖酸组成与含量分析[J].西北农林科技大学学报(自然科学版),2011,39(10):163-170.LIANG J,GUO Y,LIU Y L,et al.Analysis of contents and constituents of sugar and organic acid in different apple cultivars[J].Journal of Northwest A&F University(Natural Science Edition),2011,39(10):163-170.
[16]GARG N,SINGLA P.Stimulation of nitrogen fixation and trehalose biosynthesis by naringenin(Nar)and arbuscular mycorrhiza(AM)in chickpea under salinity stress[J].Plant Growth Regulation,2016,80(2):5-22.
[17]ILHAN S,OZDEMIR F,BOR M.Contribution of trehalose biosynthetic pathway to drought stress tolerance of Capparis ovata Desf[J].Plant Biology,2015,17(2):402-407.
[18]LOPEZ M,TEJERA N A,IRIBARNE C,et al.Trehalose and trehalase in root nodules of Medicagotruncatulaand Phaseolus vulgaris in response to salt stress[J].Physiologia Plantarum,2008,134(4):575-582.
[19]杨方威.脱落酸(ABA)对大平顶枣采后生理特性及贮藏品质的影响[D].辽宁锦州:渤海大学,2015.YANG F W.Effect of abscisic acid on postharvest physiology and storage of Chaoyang Dapingding Jujube(Ziziphus jujuba Mill.)[D].Jinzhou Liaoning:Bohai University,2015.
[20]谷继成,王有年,姚允聪.苹果幼树对干旱信号ABA响应机理的研究进展[J].北京农学院学报,1996,11(2):90-109.GU J CH,WANG Y N,YAO Y C.Research progress on response mechanism of apple young tree to drought signal ABA[J].Journal of Beijing University of Agricultural,1996,11(2):90-109.
[21]LIU Y M,ZHANG X Z,TRAN H,et al.Assessment of drought tolerance of 49switchgrass(Panicum virgatum)genotypes using physiological and morphological parameters[J].Biotechnology for Biofuels,2015,8:148-152.
[22]ITURRIAGA G,SUAREZ R,NOVA-FRANCO B.Trehalose metabolism:from osmopmtection to signaling[J].International Journal of Molecular Sciences,2009,10:3793-3810.
[23]NAYYAR H,BAINS T,KUMAR S.Low temperature induced floral abortion in chickpea:relationship to abscisic acid and cryoprotectants in reproductive organs[J].Environmental and Experimental Botany,2005,53(1):39-47.
[24]陈素丽,彭瑜,周华,等.植物海藻糖代谢及海藻糖-6-磷酸信号研究进展[J].植物生理学报,2014,50(3):233-242.CHEN S L,PENG Y,ZHOU H,et al.Research advances in trehalose metabolism and trehalose-6-phosphate signaling in plants[J].Plant Physiology Journal,2014,50(3):233-242.
[25]TIAN C J,ZUO Z L,QIU J L.Identification and characterization of ABA-responsive microRNAs in rice[J].Journal of Genetics and Genomics,2015,42(7):393-402.
[26]MULLER J,BOLLET T,WIEMKEN A.Trehalose affects sucrose synthase and invertase activities in soybean(Glycine max L.Merr.)roots[J].Journal of Plant Physiology,1998,153(1/2):255-257.
[27]BARRAZA A,ESTRADA-NAVARRETE G,RODRIGU-EZ-ALEGRIA M E,et al.Down-regulation of PvTRE1enhances nodule biomass and bacteroid number in the common bean[J].New Phytologist,2013,197(1):194-206.
[28]YADAV UP,IVAKOV A,FEIL R,et al.The sucrose-trehalose 6-phosphate(Tre6P)nexus:specificity and mechanisms of sucrose signalling by Tre6P[J].Journal of Experimental Botany,2014,65(4):1051-1068.