不同预处理方法对连翘种子萌发的影响
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摘要
连翘种皮僵硬,自然条件下发芽时间长,出苗率低,严重影响其种子繁育。本文采用不同pH值、高锰酸钾、吲哚乙酸、过氧化氢四种化学方法对连翘种子进行预处理,为提高其种子萌发率提供依据。实验结果表明,适宜的预处理对连翘种子萌发有促进作用。pH=8的碱性溶液预处理后连翘种子发芽率约高于对照组21%,发芽指数为对照组的1.14倍。低浓度的高锰酸钾、过氧化氢、吲哚乙酸溶液预处理促进种子萌发,高浓度则抑制种子萌发。0.1 g/L的高锰酸钾溶液预处理后对连翘种子的发芽能力提高最大,发芽率、发芽势、发芽指数分别提高了31.58%、61.67%、37.69%。0.1 g/L的吲哚乙酸溶液预处理后连翘种子发芽率高于对照组26.32%,发芽势高于对照组53.33%,发芽指数高于对照组32.68%。1%的过氧化氢预处理后连翘种子发芽率高于对照组24.74%,发芽势高于对照组31.68%,发芽指数高于对照组26.08%。
This study focus on improving the germination rate of seeds of Forsythia suspensa, which has rigid seed coat, long germination time under natural conditions. Four chemical methods including pH value, potassium permanganate, IAA and hydrogen peroxide were used to pretreat the Forsythia suspensa seeds, providing a basis for improving the seed germination rate. The germination rate of Forsythia suspensa seeds pretreated with pH=8 was about21% higher than the control group, and the germination index was 1.14 times higher than the control group. Low concentration of potassium permanganate, hydrogen peroxide and IAA solution pretreatment promoted seed germination, high concentration inhibited seed germination. After pretreatment with 0.1 g/L potassium permanganate solution, the germination capacity of Forsythia suspensa seeds was improved to the maximum, and the germination rate,germination energy and germination index were increased by 31.58%, 61.67% and 37.69%, respectively.The germination rate of Forsythia suspensa seeds was 26.32% higher, the germination energy was 53.33% higher and the germination index was 32.68% higher than the control. After 1% hydrogen peroxide pretreatment, the germination rate of the Forsythia suspensa seeds was 24.74% higher, the germination energy was 31.68% higher, and the germination index was 26.08% higher than the control.
This study focus on improving the germination rate of seeds of Forsythia suspensa, which has rigid seed coat, long germination time under natural conditions. Four chemical methods including pH value, potassium permanganate, IAA and hydrogen peroxide were used to pretreat the Forsythia suspensa seeds, providing a basis for improving the seed germination rate. The germination rate of Forsythia suspensa seeds pretreated with pH=8 was about21% higher than the control group, and the germination index was 1.14 times higher than the control group. Low concentration of potassium permanganate, hydrogen peroxide and IAA solution pretreatment promoted seed germination, high concentration inhibited seed germination. After pretreatment with 0.1 g/L potassium permanganate solution, the germination capacity of Forsythia suspensa seeds was improved to the maximum, and the germination rate,germination energy and germination index were increased by 31.58%, 61.67% and 37.69%, respectively.The germination rate of Forsythia suspensa seeds was 26.32% higher, the germination energy was 53.33% higher and the germination index was 32.68% higher than the control. After 1% hydrogen peroxide pretreatment, the germination rate of the Forsythia suspensa seeds was 24.74% higher, the germination energy was 31.68% higher, and the germination index was 26.08% higher than the control.
引文
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[14]谢文洁,陈训.不同pH处理对贯叶连翘及贵州小连翘种子萌发的影响[J].贵州科学,2012,30(3):76-79.
[15]黄丹,许岳香,胡海波.植物种子休眠原因与机理的研究进展[J].亚热带植物科学,2010,39(2):78-83.
[16]胡真明,张雅君,沈天文,等.盐胁迫对贯叶连翘种子萌发及幼苗POD的影响[J].常州理工学院学报,2016,30(4):120-124.
[17]翟书华,杨晓虹.不同激素处理对海菜花种子萌发特性的影响[J].种子,2017,36(8):72-74.
[18]杨俊年,胡廷章,秦刚,等.两种因子对水稻陈年种子萌发的影响[J].吉林农业科学,2013,38(2):21-26.
[2]赵燕,李安平,孙斌,等.连翘种子质量标准研究[J].园艺与种苗,2014(4):29-33.
[3]王贵元.不同浓度6-BA和GA3处理对豆梨实生苗生长的影响[J].吉林农业科学,2015,40(4):87-89.
[4]牛芳芳,唐秀光,任士福,等.连翘种子萌发特性研究[J].河北林果研究,2013,28(2):150-153.
[5]董诚明,陈随清,冯卫生,等.连翘种子萌发特性研究[J].河南中医学院学报,2005,20(1):26-27.
[6]杨文秀,杨忠仁,李红艳,等.促进植物种子萌发及解除休眠方法研究[J].内蒙古大学学报,2008(2):221-224.
[7]Williamson G B,Richardson D.Bioassays for allelopathy:Measuring treatment responses with in dependent controls[J].JChem Ecol,1998,14(1):181-187.
[8]陈伟,马绍宾,陈宏伟.种子休眠类型及其破除方法概述[J].安徽农业科学,2009,37(33):16237-16239.
[9]耿艳秋,李大勇,吴莹,等.低温胁迫对玉米萌发出苗期生理生化指标的影响[J].东北农业科学,2016,41(6):11-15.
[10]唐华江,赵丽丽,陈超,等.不同处理方法对2种雀稗属牧草种子萌发的影响[J].种子,2017,36(2):73-76.
[11]曹红委.种子休眠及破除休眠的方法[J].河南农业,2011(19):43.
[12]李惠民.贯叶连翘种子休眠特性及光对种子萌发的影响[J].西安工程大学学报,2012(6):788-790.
[13]青格乐,王玉芝,张琼琳,等.植物种子休眠及破除方法研究进展[J].安徽农业科学,2013,41(11):4715-4716.
[14]谢文洁,陈训.不同pH处理对贯叶连翘及贵州小连翘种子萌发的影响[J].贵州科学,2012,30(3):76-79.
[15]黄丹,许岳香,胡海波.植物种子休眠原因与机理的研究进展[J].亚热带植物科学,2010,39(2):78-83.
[16]胡真明,张雅君,沈天文,等.盐胁迫对贯叶连翘种子萌发及幼苗POD的影响[J].常州理工学院学报,2016,30(4):120-124.
[17]翟书华,杨晓虹.不同激素处理对海菜花种子萌发特性的影响[J].种子,2017,36(8):72-74.
[18]杨俊年,胡廷章,秦刚,等.两种因子对水稻陈年种子萌发的影响[J].吉林农业科学,2013,38(2):21-26.