茉莉酸甲酯调控防御酶活性诱导猕猴桃果实抗采后软腐病
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摘要
以‘金魁’猕猴桃果实为试验材料,研究茉莉酸甲酯(methyl jasmonate,MeJA)调控防御酶活性抗猕猴桃采后软腐病的效应。测定了MeJA对猕猴桃软腐病病斑直径、软腐病菌Botryosphaeria dothidea抑菌作用及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和多酚氧化酶(PPO)等防御酶活性的影响。结果表明:在0.001～10 mmol/L浓度范围内,MeJA对猕猴桃软腐病菌B.dothidea的抑制作用随浓度升高而增强;MeJA对猕猴桃果实最佳诱导浓度和熏蒸时间分别为0.1 mmol/L和24 h,其诱导效果分别为26.01%和26.85%;猕猴桃果实经0.1 mmol/L MeJA熏蒸处理24 h后,SOD、POD、CAT、APX和PPO活性提高,其中SOD和POD活性分别较对照增加33.85%和61.61%,差异达显著水平(P<0.05)。以上结果暗示MeJA诱导猕猴桃果实抗采后软腐病可能与其提高防御酶活性有关。
In this study, ‘Jinkui' kiwifruits were used to investigate the effects of methyl jasmonate(MeJA) regulating the activities of defense enzymes on the induced resistance to soft rot(Botryosphaeria dothidea) of posthavest kiwifruit. The inhibition effects of MeJA against B.dothidea and the disease spot diameters as well as the activities of the related defense enzymes, including superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), ascorbic acid peroxidase(APX) and polyphenol oxidase(PPO), were measured. The results showed that the inhibition effects of MeJA on B.dothidea increased with increasing concentration in the range of 0.001-10 mmol/L. In MeJA induction experiments, it was found that the best MeJA treatment concentration and duration were 0.1 mmol/L and 24 h, with an inductive effect of 26.01% and 26.85%, respectively. The activities of SOD, CAT, POD, APX and PPO in fruits treated with 0.1 mmol/L MeJA for 24 h were increased and the SOD and POD activities were significantly enhanced by 33.85% and 61.61% compared with those of the control(P<0.05). These results indicated that the increased activities of defense enzymes might be involved in the resistant reactions of MeJA to soft rot in postharvest kiwifruits.
In this study, ‘Jinkui' kiwifruits were used to investigate the effects of methyl jasmonate(MeJA) regulating the activities of defense enzymes on the induced resistance to soft rot(Botryosphaeria dothidea) of posthavest kiwifruit. The inhibition effects of MeJA against B.dothidea and the disease spot diameters as well as the activities of the related defense enzymes, including superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), ascorbic acid peroxidase(APX) and polyphenol oxidase(PPO), were measured. The results showed that the inhibition effects of MeJA on B.dothidea increased with increasing concentration in the range of 0.001-10 mmol/L. In MeJA induction experiments, it was found that the best MeJA treatment concentration and duration were 0.1 mmol/L and 24 h, with an inductive effect of 26.01% and 26.85%, respectively. The activities of SOD, CAT, POD, APX and PPO in fruits treated with 0.1 mmol/L MeJA for 24 h were increased and the SOD and POD activities were significantly enhanced by 33.85% and 61.61% compared with those of the control(P<0.05). These results indicated that the increased activities of defense enzymes might be involved in the resistant reactions of MeJA to soft rot in postharvest kiwifruits.
引文
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[6] 邱德文. 我国植物免疫诱导技术的研究现状与趋势分析[J]. 植物保护, 2016, 42(5): 10-14.
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[12] SHADMANI N, AHMAD S H, SAARI N, et al. Chilling injury incidence and antioxidant enzyme activities of Carica papaya L.‘Frangi’ as influenced by postharvest hot water treatment and storage temperature[J]. Postharvest Biology and Technology, 2015, 99: 114-119.
[13] SHI Junyan, GAO Lipu, ZUO Jinhua, et al. Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life, enhances antioxidant enzyme activity, and inhibits chlorophyll-degradation[J]. Postharvest Biology and Technology, 2016, 116: 98-104.
[14] 曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007.
[15] GLOWACZ M, ROETS N, SIVAKUMAR D. Control of anthracnose disease via increased activity of defence related enzymes in ‘Hass’ avocado fruit treated with methyl jasmonate and methyl salicylate [J].Food Chemistry,2017,234:163-167.
[16] 田世平,罗云波,王贵禧.园艺产品采后生物学基础[M].北京:科学出版社,2011:43-55,312-318.
[17] 向妙莲,赵显阳,陈明,等.茉莉酸甲酯诱导辣椒抗青枯病与活性氧代谢的关系[J].园艺学报,2017,44(10):1985-1992.
[18] 姜璐璐.茉莉酸甲酯对葡萄果实常温保鲜效果及其机理研究[D].南京:南京农业大学,2015.
[19] WANG Kaituo, LIAO Yunxia, KAN Jianquan, et al. Response of direct or priming defense against Botrytis cinerea to methyl jasmonate treatment at different concentrations in grape berries [J]. International journal of food microbiology, 2015, 194: 32-39.
[20] 郭娟华,陈明,涂起红,等.类芽孢杆菌YS-1代谢产物粗提液对柑橘青霉病的生防效果[J].植物保护学报,2013,40(6):573-574.
[21] 陆建英,杨晓明,王昶,等.不同诱抗剂防治豌豆白粉病的效果及对防御酶的影响[J].植物保护,2017,43(5):221-224.
[22] 田国忠,李怀方,裘维蕃.植物过氧化物酶研究进展[J].植物科学学报,2001,19(4):332-344.
[23] GRIGORAS A G. Catalase immobilization—A review [J]. Biochemical Engineering Journal, 2017, 117: 1-20.
[24] MENG Demei, ZHANG Yaxuan, YANG Rui, et al. Arginase participates in the methyl jasmonate-regulated quality maintenance of postharvest Agaricus bisporus fruit bodies [J]. Postharvest Biology and Technology, 2017, 132: 7-14.
[25] FAN Linlin, WANG Qing, LV Jiayu, et al. Amelioration of postharvest chilling injury in cowpea(Vigna sinensis) by methyl jasmonate (MeJA) treatments[J]. Scientia Horticulturae, 2016, 203: 95-101.
[2] ZHOU You, GONG Guoshu, CUI Yongliang, et al. Identification of Botryosphaeriaceae species causing kiwifruit rot in Sichuan Province, China [J].Plant Disease,2015,99(5):699-708.
[3] MARI M, SPADONI A, CEREDI G. Alternative technologies to control postharvest diseases of kiwifruit [J]. Stewart Postharvest Review, 2015, 11(4): 1-5.
[4] 张艳宜, 任亚梅, 宋小青, 等. CPPU 和 1-MCP 处理对采后猕猴桃抗灰霉病的影响[J]. 中国食品学报, 2017(4): 140-146.
[5] SHARMA S, PAREEK S, SAGAR N A, et al. Modulatory effects of exogenously applied polyamines on postharvest physiology, antioxidant system and shelf life of fruits: a review [J]. International Journal of Molecular Sciences,2017,18(8):1789.
[6] 邱德文. 我国植物免疫诱导技术的研究现状与趋势分析[J]. 植物保护, 2016, 42(5): 10-14.
[7] 周燮. 新发现的植物激素[M].南京: 江苏科学技术出版社, 2010.
[8] 王英珍, 程瑞, 张绍铃, 等. 采前茉莉酸甲酯(MeJA) 处理对梨果实抗病性的影响[J]. 果树学报, 2016, 33(6): 694-700.
[9] 弓德强,黄训才,黄光平,等.茉莉酸甲酯处理对采后芒果果实抗病性的影响[J].热带作物学报,2016,37(12):2294-2299.
[10] 黄晓杰, 李婧, 柴媛, 等. MeJA处理对蓝莓果实采后灰霉病的影响及机理[J]. 食品科学, 2016, 37(22): 307-312.
[11] ZAPATA P J, MARTINEZ-ESPLá A, GUILLéN F, et al. Preharvest application of methyl jasmonate (MeJA) in two plum cultivars. 2. Improvement of fruit quality and antioxidant systems during postharvest storage[J]. Postharvest Biology and Technology, 2014, 98: 115-122.
[12] SHADMANI N, AHMAD S H, SAARI N, et al. Chilling injury incidence and antioxidant enzyme activities of Carica papaya L.‘Frangi’ as influenced by postharvest hot water treatment and storage temperature[J]. Postharvest Biology and Technology, 2015, 99: 114-119.
[13] SHI Junyan, GAO Lipu, ZUO Jinhua, et al. Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life, enhances antioxidant enzyme activity, and inhibits chlorophyll-degradation[J]. Postharvest Biology and Technology, 2016, 116: 98-104.
[14] 曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007.
[15] GLOWACZ M, ROETS N, SIVAKUMAR D. Control of anthracnose disease via increased activity of defence related enzymes in ‘Hass’ avocado fruit treated with methyl jasmonate and methyl salicylate [J].Food Chemistry,2017,234:163-167.
[16] 田世平,罗云波,王贵禧.园艺产品采后生物学基础[M].北京:科学出版社,2011:43-55,312-318.
[17] 向妙莲,赵显阳,陈明,等.茉莉酸甲酯诱导辣椒抗青枯病与活性氧代谢的关系[J].园艺学报,2017,44(10):1985-1992.
[18] 姜璐璐.茉莉酸甲酯对葡萄果实常温保鲜效果及其机理研究[D].南京:南京农业大学,2015.
[19] WANG Kaituo, LIAO Yunxia, KAN Jianquan, et al. Response of direct or priming defense against Botrytis cinerea to methyl jasmonate treatment at different concentrations in grape berries [J]. International journal of food microbiology, 2015, 194: 32-39.
[20] 郭娟华,陈明,涂起红,等.类芽孢杆菌YS-1代谢产物粗提液对柑橘青霉病的生防效果[J].植物保护学报,2013,40(6):573-574.
[21] 陆建英,杨晓明,王昶,等.不同诱抗剂防治豌豆白粉病的效果及对防御酶的影响[J].植物保护,2017,43(5):221-224.
[22] 田国忠,李怀方,裘维蕃.植物过氧化物酶研究进展[J].植物科学学报,2001,19(4):332-344.
[23] GRIGORAS A G. Catalase immobilization—A review [J]. Biochemical Engineering Journal, 2017, 117: 1-20.
[24] MENG Demei, ZHANG Yaxuan, YANG Rui, et al. Arginase participates in the methyl jasmonate-regulated quality maintenance of postharvest Agaricus bisporus fruit bodies [J]. Postharvest Biology and Technology, 2017, 132: 7-14.
[25] FAN Linlin, WANG Qing, LV Jiayu, et al. Amelioration of postharvest chilling injury in cowpea(Vigna sinensis) by methyl jasmonate (MeJA) treatments[J]. Scientia Horticulturae, 2016, 203: 95-101.