血清可溶性白细胞介素2受体与乙型病毒性肝炎患者细胞免疫功能及肝功能的关系
|
摘要
目的探讨血清中可溶性白细胞介素2受体水平与乙型肝炎患者细胞免疫功能及肝功能的关系。方法选择2015年6月-2016年6月在本院行治疗的乙型肝炎患者120例,采用酶联免疫吸附法检测患者血液中可溶性白细胞介素2受体的水平,流失细胞仪检测外周血T细胞亚群,全自动生化仪检测患者肝功能,根据肝功结果和HBsAg结果分为肝功能正常的HBsAg(+)观察组,肝功能异常伴HBsAg(+)对照组,探讨血清中可溶性白细胞介素2受体与患者细胞免疫及肝功能的关系。结果观察组血清Treg细胞水平明显低于对照组,Th17细胞水平明显高于对照组;观察组血清IL-2、IL-6、sIL-2R水平均明显高于对照组,差异均有统计学意义(P<0.05)。Logistic回归分析结果提示,IL-6、sIL-2R水平、Treg细胞含量是肝损害的独立危险因素(P<0.05)。结论血清中可溶性白细胞介素-2与患者免疫功能和肝功能异常密切相关。
Objective To investigate the relationship of serum soluble interleukin 2 receptor level with cell immune function and liver function in patients with Viral hepatitis B. Methods A total of 120 cases in our hospital from July 2014 to July 2015 year were selected for blood test. The level of soluble interleukin-2 receptor in the blood of patients was detected by enzyme-linked immunosorbent assay(ELISA). The peripheral blood T cell subsets were detected by cytometry, and the liver function of patients was detected by automatic biochemical analyzer. According to the results of liver function and HBsAg, the patients were divided into observation group with normal liver function and control group with abnormal liver function and HBsAg(+).To investigate the relationship of serum soluble interleukin-2 receptor with cellular immunity and liver function in patients. Results The level of serum Treg cells in the observation group was significantly lower than that in the control group, and the level of Th17 cells in the observation group was significantly higher than that in the control group, with the differences statistically significant(P>0.05). Logistic regression analysis showed that IL-6, sIL-2 R and Treg cell content were independent risk factors for liver injury(P<0.05). Conclusion The serum soluble white blood cells were closely related to the immune function and liver function abnormality.
Objective To investigate the relationship of serum soluble interleukin 2 receptor level with cell immune function and liver function in patients with Viral hepatitis B. Methods A total of 120 cases in our hospital from July 2014 to July 2015 year were selected for blood test. The level of soluble interleukin-2 receptor in the blood of patients was detected by enzyme-linked immunosorbent assay(ELISA). The peripheral blood T cell subsets were detected by cytometry, and the liver function of patients was detected by automatic biochemical analyzer. According to the results of liver function and HBsAg, the patients were divided into observation group with normal liver function and control group with abnormal liver function and HBsAg(+).To investigate the relationship of serum soluble interleukin-2 receptor with cellular immunity and liver function in patients. Results The level of serum Treg cells in the observation group was significantly lower than that in the control group, and the level of Th17 cells in the observation group was significantly higher than that in the control group, with the differences statistically significant(P>0.05). Logistic regression analysis showed that IL-6, sIL-2 R and Treg cell content were independent risk factors for liver injury(P<0.05). Conclusion The serum soluble white blood cells were closely related to the immune function and liver function abnormality.
引文
[1] 刘园,贺鹍鹏,强艳,等.某体检人群非酒精性脂肪肝的现状及其与高脂血症、肝功能异常的关系[J].宁夏医科大学学报,2015,37(3):267-271.
[2] 齐育英,林振忠,明德松,等.IL-6、IL-10、IL-17在慢性乙型病毒性肝炎患者血中水平分析[J].中国免疫学杂志,2013,29(11):1177-1180.
[3] 吴婷.非酒精性脂肪性肝病合并病毒性肝炎小鼠模型的建立及其免疫学损伤机制的研究[D].武汉:华中科技大学,2014.
[4] 郑金萍,朱叶珊,李健,等.保肝降酶饮联合恩替卡韦片对慢性乙型病毒性肝炎患者肝功能及炎症细胞因子的影响[J].河北中医,2015,37(9):1318-1320.
[5] 马秀丽,陈锐.高校新生体检肝功能异常情况研究[J].中国保健营养,2016,26(6):42-42.
[6] 温婷,吕谦,廖大忠,等.细胞免疫治疗对慢性乙型病毒性肝炎患者HBV-DNA的影响观察[J].基层医学论坛,2015(4):457-458.
[7] 曾跃红,田海清,王新华,等.乙型病毒性肝炎临床转归与机体细胞免疫功能状况的相关性研究[J].中国医药导报,2015,12(5):8-11,21.
[8] 赵晓春,郭熙清,刘书宏,等.慢性乙型病毒性肝炎患者抗病毒治疗中细胞免疫功能的改变[J].安徽医药,2015(1):108-110.
[9] 王晶慧,吴标,黄玲,等.不同临床阶段乙型肝炎患者外周CD8+T淋巴细胞免疫记忆应答水平的比较[J].肝脏,2013,18(6):369-373.
[10] 王海燕,王煜,王琦,等.替比夫定对慢性乙型肝炎患者血清sIL-2R及球蛋白水平的影响[J].宁夏医科大学学报,2013,35(2):161-163.
[11] 邢凌翔,王晓霖,程海军,等.异甘草酸镁对乙型肝炎患者外周血 sIL-2R 的影响[J].肝脏,2014,19(12):995-996.
[12] 任小交.DHAV-1感染DEF和鸭的细胞因子IL-2、IL-2-B、IFN-γ基因mRNA表达水平研究[D].武汉:华中农业大学,2014.
[13] 田庆刚,彭健,张阳德,等.肝移植术后血清可溶性白细胞介素-2受体和胆汁白细胞介素-6的监测及意义[J].中华实验外科杂志,2015,32(5):1153-1155.
[14] 陈晓春,李宁,朱坚胜,等.前列地尔注射液辅助治疗对重型肝炎合并肝肾综合征患者血清TNF-α、β2-MG及sIL-2 R的影响[J].中国生化药物杂志,2016,36(6):73-75.
[15] 高健,王桂平,赵春燕,等.慢性乙肝患者抗病毒治疗血清IL-10、IL-12、sIL-2R、TNF水平变化的临床观察[J].河北医药,2013,35(14):2132-2133.
[2] 齐育英,林振忠,明德松,等.IL-6、IL-10、IL-17在慢性乙型病毒性肝炎患者血中水平分析[J].中国免疫学杂志,2013,29(11):1177-1180.
[3] 吴婷.非酒精性脂肪性肝病合并病毒性肝炎小鼠模型的建立及其免疫学损伤机制的研究[D].武汉:华中科技大学,2014.
[4] 郑金萍,朱叶珊,李健,等.保肝降酶饮联合恩替卡韦片对慢性乙型病毒性肝炎患者肝功能及炎症细胞因子的影响[J].河北中医,2015,37(9):1318-1320.
[5] 马秀丽,陈锐.高校新生体检肝功能异常情况研究[J].中国保健营养,2016,26(6):42-42.
[6] 温婷,吕谦,廖大忠,等.细胞免疫治疗对慢性乙型病毒性肝炎患者HBV-DNA的影响观察[J].基层医学论坛,2015(4):457-458.
[7] 曾跃红,田海清,王新华,等.乙型病毒性肝炎临床转归与机体细胞免疫功能状况的相关性研究[J].中国医药导报,2015,12(5):8-11,21.
[8] 赵晓春,郭熙清,刘书宏,等.慢性乙型病毒性肝炎患者抗病毒治疗中细胞免疫功能的改变[J].安徽医药,2015(1):108-110.
[9] 王晶慧,吴标,黄玲,等.不同临床阶段乙型肝炎患者外周CD8+T淋巴细胞免疫记忆应答水平的比较[J].肝脏,2013,18(6):369-373.
[10] 王海燕,王煜,王琦,等.替比夫定对慢性乙型肝炎患者血清sIL-2R及球蛋白水平的影响[J].宁夏医科大学学报,2013,35(2):161-163.
[11] 邢凌翔,王晓霖,程海军,等.异甘草酸镁对乙型肝炎患者外周血 sIL-2R 的影响[J].肝脏,2014,19(12):995-996.
[12] 任小交.DHAV-1感染DEF和鸭的细胞因子IL-2、IL-2-B、IFN-γ基因mRNA表达水平研究[D].武汉:华中农业大学,2014.
[13] 田庆刚,彭健,张阳德,等.肝移植术后血清可溶性白细胞介素-2受体和胆汁白细胞介素-6的监测及意义[J].中华实验外科杂志,2015,32(5):1153-1155.
[14] 陈晓春,李宁,朱坚胜,等.前列地尔注射液辅助治疗对重型肝炎合并肝肾综合征患者血清TNF-α、β2-MG及sIL-2 R的影响[J].中国生化药物杂志,2016,36(6):73-75.
[15] 高健,王桂平,赵春燕,等.慢性乙肝患者抗病毒治疗血清IL-10、IL-12、sIL-2R、TNF水平变化的临床观察[J].河北医药,2013,35(14):2132-2133.