茜素红S对扁吻鱼幼鱼的急性毒性试验
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摘要
为了解茜素红S对扁吻鱼(Aspiorhynchus laticeps)幼鱼的急性毒性,在水温10℃下,采用半静态法对体重范围(5.8±0.6)g的扁吻鱼幼鱼进行了试验。结果表明,茜素红S浓度为400 mg/L时,96 h死亡率为0;当茜素红浓度为900 mg/L时,72 h全部死亡;茜素红S对扁吻鱼幼鱼的24、48、72、96 h的半致死浓度分别为788、718、651、618 mg/L,安全质量浓度为179 mg/L。说明茜素红S对扁吻鱼幼鱼的毒性为低毒性,适合其标记放流。实际工作中,建议选用浓度179～400 mg/L作标记,在保证存活率的基础上,取得较好的标记效果。
In order to understand the acute toxicity of alizarin red S to Aspiorhynchus laticeps juvenile fish,the A.laticeps juvenile fish with body weight of(5.8±0.6)g was studied at 10℃ using a semi-static test method.The results showed that,when the concentration of alizarin red S was 400 mg/L,the mortality rate at 96 hours was 0;when the concentration of alizarin red S was 900 mg/L,all died within 72 hours;result showed the semilethal concentrations of alizarin red S on Aspiorhynchus laticeps at 24 hours,48 hours,72 hours,and 96 hours were respectively 788,718,651 and 618 mg/L.The safe mass concentration was 179 mg/L.Our results indicated that alizarin red S had low toxicity to Aspiorhynchus laticeps,which was suitable for tagging and releasing of Aspiorhynchus laticep.In the practical work,on the basis of the guarantee survival rate,our results suggested selecting concentrations between 179 mg/L and 400 mg/L as tags to get better marks.
In order to understand the acute toxicity of alizarin red S to Aspiorhynchus laticeps juvenile fish,the A.laticeps juvenile fish with body weight of(5.8±0.6)g was studied at 10℃ using a semi-static test method.The results showed that,when the concentration of alizarin red S was 400 mg/L,the mortality rate at 96 hours was 0;when the concentration of alizarin red S was 900 mg/L,all died within 72 hours;result showed the semilethal concentrations of alizarin red S on Aspiorhynchus laticeps at 24 hours,48 hours,72 hours,and 96 hours were respectively 788,718,651 and 618 mg/L.The safe mass concentration was 179 mg/L.Our results indicated that alizarin red S had low toxicity to Aspiorhynchus laticeps,which was suitable for tagging and releasing of Aspiorhynchus laticep.In the practical work,on the basis of the guarantee survival rate,our results suggested selecting concentrations between 179 mg/L and 400 mg/L as tags to get better marks.
引文
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[6]刘奇.褐牙鲆标志技术与增殖放流试验研究[D].青岛:中国海洋大学,2009.
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[11]何春林,付自东,严太明,等.用荧光物质浸泡标记重口裂腹鱼仔鱼耳石[J].四川动物,2008,27(3):331-334.
[12]谢春刚,张人铭,吐尔逊,等.7种常用药物对扁吻鱼幼鱼的急性毒性试验[J].干旱区研究,2010,27(1):104-108.
[13]霍来江.茜素红S对中华倒刺鲃幼鱼的染色效果以及对其氧化应激响应[D].武汉:华中农业大学,2015.
[14]吴邦灿,费龙.现代环境监测技术[M].北京:中国环境科学出版社,1999:252-254.
[15]中国国家标准化管理委员会.GB/T 21281-2007危险化学品鱼类急性毒性分级试验方法[S].北京:中国标准出版社,2008.
[2]乐佩琦,陈宣瑜.中国濒危动物红皮书:鱼类[M].北京:科学出版社,1998:153-155.
[3]郝卓然,梁利群,常玉梅,等.扁吻鱼幼鱼微卫星的筛选及群体多样性分析[J].水产学杂志,2012,25(3):20-25.
[4]吴迎秋,袁强,李辉.Co/Ti O2催化剂对茜素红的降解探究[J].环保科技,2010,16(2):1-
[5]梁小双.红鳍笛鲷荧光标记技术的研究[D].西宁:广西大学,2014.
[6]刘奇.褐牙鲆标志技术与增殖放流试验研究[D].青岛:中国海洋大学,2009.
[7]Baer J,R9sch R.Mass-marking of brown trout(Salmo trutta L.)larvae by alizarin:method and evaluation of stocking[J].Journal of Applied Ichthyology,2010,24(1):44-49.
[8]欧阳斌,常剑波.荧光物质浸泡标记稀有鮈鲫和彭泽鲫仔、稚鱼[J].水生生物学报,1999,23(4):324-349.
[9]宋昭彬,曹文宣.鳡鱼仔稚鱼耳石的标记和其日轮的确证[J].水生生物学报,1999,23(6):677-682.
[10]付自东,李静,岳碧松,等.用荧光物质浸泡标记胭脂鱼仔、稚鱼耳石[J].动物学杂志,2005,40(4):60-65.
[11]何春林,付自东,严太明,等.用荧光物质浸泡标记重口裂腹鱼仔鱼耳石[J].四川动物,2008,27(3):331-334.
[12]谢春刚,张人铭,吐尔逊,等.7种常用药物对扁吻鱼幼鱼的急性毒性试验[J].干旱区研究,2010,27(1):104-108.
[13]霍来江.茜素红S对中华倒刺鲃幼鱼的染色效果以及对其氧化应激响应[D].武汉:华中农业大学,2015.
[14]吴邦灿,费龙.现代环境监测技术[M].北京:中国环境科学出版社,1999:252-254.
[15]中国国家标准化管理委员会.GB/T 21281-2007危险化学品鱼类急性毒性分级试验方法[S].北京:中国标准出版社,2008.