猕猴桃素-D的抗肺癌作用和免疫调节功能
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摘要
建立了裸鼠A549肺癌模型和小鼠Lewis肺癌模型,以环磷酰胺为阳性药,考察猕猴桃素-D对肿瘤生长的抑制作用。并以云芝肝泰胶囊为阳性药,测定了猕猴桃素-D对荷Lewis肺癌雌性小鼠的淋巴细胞增殖和NK细胞活性的影响,评价药物对细胞免疫功能的影响。结果表明,与生理盐水组相比,猕猴桃素-D在150～600 mg/kg范围内对裸鼠A549肺癌细胞和小鼠Lewis肺癌细胞的生长有明显抑制作用,且呈剂量相关性;但与环磷酰胺相比,各剂量组的抑瘤率仍较低。中、高剂量的猕猴桃素-D还可显著促进荷Lewis肺癌雌性小鼠脾淋巴细胞增殖作用,升高NK细胞活性,且在提高雌性小鼠机体免疫力方面优于云芝肝泰胶囊。结果提示猕猴桃素-D可能具有继续向抗肿瘤新药开发的潜力。
The A549 lung cancer-bearing nude mouse model and Lewis lung cancer-bearing mouse model were established to investigate the tumor growth inhibition of actinidin D with cyclophosphamide as positive drug.The effects of actinidin D on lymphocyte proliferation and NK cell activity of female mice bearing Lewis tumor were investigated with Polystictus glycopeptides capsules as positive drug.The results showed that compared with the saline group,actinidin D had a dose-related tumor growth inhibition on A549 and Lewis lung cancer cells in the concentration range of 150-600 mg/kg,while the tumor inhibitory rate of actinidin D groups was lower than that of cyclophosphamide group.The actinidin D in middle-and high-dose(300 and 600 mg/kg) group could significantly enhance lymphocyte proliferation and increase NK cell activity in Lewis cancer-bearing female mice,and this effect was superior to Polystictus glycopeptides capsules.The results suggested that actinidin D might be considered as a potent antitumor drug candidate.
The A549 lung cancer-bearing nude mouse model and Lewis lung cancer-bearing mouse model were established to investigate the tumor growth inhibition of actinidin D with cyclophosphamide as positive drug.The effects of actinidin D on lymphocyte proliferation and NK cell activity of female mice bearing Lewis tumor were investigated with Polystictus glycopeptides capsules as positive drug.The results showed that compared with the saline group,actinidin D had a dose-related tumor growth inhibition on A549 and Lewis lung cancer cells in the concentration range of 150-600 mg/kg,while the tumor inhibitory rate of actinidin D groups was lower than that of cyclophosphamide group.The actinidin D in middle-and high-dose(300 and 600 mg/kg) group could significantly enhance lymphocyte proliferation and increase NK cell activity in Lewis cancer-bearing female mice,and this effect was superior to Polystictus glycopeptides capsules.The results suggested that actinidin D might be considered as a potent antitumor drug candidate.
引文
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[3]赵雪强,王绩英,王昌明,等.三氧化二砷增强TRAIL杀伤人A549细胞的作用及机制研究[J].中国药理学通报,2011,27(3):415-418.
[4]Curran WJ,Scott CB,Langer CJ,et a1.Long-term benefitis observed in a phase III comparison of sequential vsconcurrent chemo-radiation for patients with unresected stageIII NSCLC:RTOG 9410[C]//2003 ASCO Annual Meeting.Proc Am Society Clin Oncol 22,2003:(abstr 2499).
[5]周志群.猕猴桃根醇提物及吴茱萸碱单体调节SW480细胞CCR7表达的研究[D].中南大学硕士学位论文,2009.
[6]李昊,杨慧萍,杨凡,等.藤梨根对胃癌细胞抑制作用的实验研究[J].河北中医,2004,26(4):314-315.
[7]Xu YX,Xiang ZB,Jin YS,et al.Two new triterpenoids fromthe roots of Actinidia chinensis[J].Fitoterapia,2010,81(7):920-924.
[8]楼丽君,吕定量,胡增仁,等.猕猴桃根抗肿瘤作用研究[J].中国药理学通报,2007,23(6):808-811.
[9]李军霞,杲海霞,陈雪彦,等.戊地昔布抑制Lewis肿瘤生长的作用[J].中国药理学通报,2006,22(8):998-1001.