稳心颗粒对兔心室肌晚钠电流影响的研究
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摘要
目的:应用微电极阵列(MEA)技术研究稳心颗粒对兔心室肌晚钠电流(INaL)的影响。方法:新西兰大白兔50只,随机分成5组,对照组(n=10),心衰组(n=10),心衰+稳心颗粒A组(1 g/L,n=10),心衰+稳心颗粒B组(2 g/L,n=10),心衰+稳心颗粒C组(4 g/L,n=10)。迅速开胸取出心脏进行langendroff灌流,用柔性电极贴附左心室,记录场电位时限(fAPD)等电生理信号的变化。结果:与对照组相比,心衰组兔心室肌fAPD由(347.13±12.41)ms延长至(392.52±13.50)ms,激动传导速度(ECV)由(0.68±0.06)m/s减慢至(0.55±0.08)m/s,心率(HR)由(104±7.23)bpm加快至(126±9.52)bpm(均P<0.05)。与心衰组相比,心衰+稳心颗粒A、B、C组fAPD分别由(392.52±13.50)ms缩短至(369.83±10.72)ms、(348.13±12.56)ms、(331.26±9.37)ms;ECV分别由(0.55±0.08)m/s减慢至(0.45±0.10)m/s、(0.43±0.08)m/s、(0.42±0.07)m/s;HR分别由(126±9.52)bpm减慢至(105±8.42)bpm、(97±6.70)bpm、(94±8.62)bpm(均P<0.05)。但稳心颗粒A、B、C组之间ECV和HR变化无明显差异(P>0.05)。结论:稳心颗粒是一种安全有效的抗心律失常中药,对INaL的抑制作用是其发挥抗心律失常作用的主要机制之一。
Objective:To explore the effect of Wenxin Granule on the late sodium current(INaL) in rabbit ventricular muscle by microelectrode array(MEA)technology.Methods:Fixty rabbits of either sex were randomly divided into 5 groups:control group(n=10),heart failure group(n=10),heart failure+Wenxin Granule A group(1 g/L,n=10),heart failure+Wenxin Granule B group(2 g/L,n=10),heart failure+Wenxin Granul C group(4 g/L,n=10).The heart was perfused with modified Tyrode′s solution after removed.Flexible electrodes were attached to the left ventricle,and we recorded the changes of electrophysiological signals.Results:In the heart failure groups,fAPD prolonged from(347.13 ±12.41)ms to(389.52 ±13.50)ms,ECV decreased from(0.68±0.06)m/s to(0.55±0.08)m/s,whlie HR increased from(104±7.23)bpm to(126±9.52)bpm compared with the control group′s(above all P<0.05).In the heart failure group+Wenxin Granule A,B,C groups,fAPD shortened from(389.52 ±13.50)ms to(367.83 ±10.72)ms,(348.13 ±12.56)ms and(331.26 ±9.37)ms respectively.ECV decreased from(0.55±0.08)m/s to(0.45±0.10)m/s,(0.43±0.08)m/s and(0.42±0.07)m/s respectively,while HR decreased from(126±9.52)bpm to(105±8.42)bpm,(97±6.70)bpm and(94±8.62)bpm(above all P<0.05).However,there were no significant differences in the change of ECV or HR amongst Wenxin Granule A,B and C groups(P>0.05).Conclusion:Wenxin Granule is a safe and effective antiarrhythmic traditional Chinese medicine,and the inhibition of INaL is one of the main mechanisms of its role in antiarrhythmic effect.
Objective:To explore the effect of Wenxin Granule on the late sodium current(INaL) in rabbit ventricular muscle by microelectrode array(MEA)technology.Methods:Fixty rabbits of either sex were randomly divided into 5 groups:control group(n=10),heart failure group(n=10),heart failure+Wenxin Granule A group(1 g/L,n=10),heart failure+Wenxin Granule B group(2 g/L,n=10),heart failure+Wenxin Granul C group(4 g/L,n=10).The heart was perfused with modified Tyrode′s solution after removed.Flexible electrodes were attached to the left ventricle,and we recorded the changes of electrophysiological signals.Results:In the heart failure groups,fAPD prolonged from(347.13 ±12.41)ms to(389.52 ±13.50)ms,ECV decreased from(0.68±0.06)m/s to(0.55±0.08)m/s,whlie HR increased from(104±7.23)bpm to(126±9.52)bpm compared with the control group′s(above all P<0.05).In the heart failure group+Wenxin Granule A,B,C groups,fAPD shortened from(389.52 ±13.50)ms to(367.83 ±10.72)ms,(348.13 ±12.56)ms and(331.26 ±9.37)ms respectively.ECV decreased from(0.55±0.08)m/s to(0.45±0.10)m/s,(0.43±0.08)m/s and(0.42±0.07)m/s respectively,while HR decreased from(126±9.52)bpm to(105±8.42)bpm,(97±6.70)bpm and(94±8.62)bpm(above all P<0.05).However,there were no significant differences in the change of ECV or HR amongst Wenxin Granule A,B and C groups(P>0.05).Conclusion:Wenxin Granule is a safe and effective antiarrhythmic traditional Chinese medicine,and the inhibition of INaL is one of the main mechanisms of its role in antiarrhythmic effect.
引文
[1] Maier L S,Sossalla S.The late Na current as a therapeutic target:Where are we? [J].Journal of Molecular and Cellular Cardiology,2013,61:44-50.
[2] 李瑾,唐海沁,李结华.稳心颗粒抗心律失常的Meta 分析[J].中国循证心血管医学杂志,2011,3(2):84-88.
[3] 梁万宁,张晓梅,荣玉玺,等.稳心颗粒治疗慢性心力衰竭患者室性心律失常的临床观察[J].中华心血管病杂志,2005,33(10):884.
[4] 陈文韬,黄从新,郭凯,等.稳心颗粒对大鼠心室肌细胞跨膜动作电位的影响[J].中国心脏起搏与心电生理杂志,2010,24(4):341-343.
[5] 赵涛,赵步长,伍海勤,等.中药稳心颗粒抗心律失常的离子通道作用机制研究[J].中医临床研究,2013,5(24):105-106.
[6] 刘明鑫,权力,连志明,等.步长稳心颗粒对心力衰竭兔心室肌电生理特性的影响[J].中国心脏起搏与心电生理杂志,2012,26(6):543-546.
[7] 孙娟,黄焰,张玲,等.应用微电极芯片技术评价抗心房颤动药物对兔右心房快速起搏模型的影响[J].中国循环杂志,2014(8):639-642.
[8] 欧阳松,张晓雅,庄妤婷,等.鞣酸对大鼠心室肌场电位时限及兴奋传导速度影响的研究[J].中华心律失常学杂志,2016,20(5):430-434.
[9] 段亚琦,唐明,梁华敏.微电极矩阵研究小鼠胚胎心脏电生理活动[J].生理学报,2006,58(1):65-70.
[10] Sun J,Yan H,Wugeti N,et al.Microelectrode array measurement of potassium ion channel remodeling on the field action potential duration in rapid atrial pacing rabbits model [J].Int J Clin Exp Med,2015,8(1):249.
[11] Banyasz T,Szentandrassy N,Magyar J,et al.An emerging antiarrhythmic target:late sodium current[J].Curr Pharm Des,2015,21(8):1073-1090.
[12] Xue X,Guo D,Sun H,et al.Wenxin Keli suppresses ventricular triggered arrhythmias via selective inhibition of late sodium current[J].Pacing Clin Electrophysiol,2013,36(6):732-740.
[13] Minoura Y,Panama BK,Nesterenko VV,et al.Effect of Wenxin Keli and quinidine to suppress arrhythmo- genesis in an experimental model of Brugada syndrome[J].Heart Rhythm,2013,10(7):1054-1062.
[14] Kalifa J,Avula UM.The Chinese herb extract Wenxin Keli:atrial selectivity from the Far East[J].Heart Rhythm,2012,9(1):132-133.
[15] 郭继鸿.心脏的传导性及异常[J].临床心电学杂志,2006,15(6):450-461.
[2] 李瑾,唐海沁,李结华.稳心颗粒抗心律失常的Meta 分析[J].中国循证心血管医学杂志,2011,3(2):84-88.
[3] 梁万宁,张晓梅,荣玉玺,等.稳心颗粒治疗慢性心力衰竭患者室性心律失常的临床观察[J].中华心血管病杂志,2005,33(10):884.
[4] 陈文韬,黄从新,郭凯,等.稳心颗粒对大鼠心室肌细胞跨膜动作电位的影响[J].中国心脏起搏与心电生理杂志,2010,24(4):341-343.
[5] 赵涛,赵步长,伍海勤,等.中药稳心颗粒抗心律失常的离子通道作用机制研究[J].中医临床研究,2013,5(24):105-106.
[6] 刘明鑫,权力,连志明,等.步长稳心颗粒对心力衰竭兔心室肌电生理特性的影响[J].中国心脏起搏与心电生理杂志,2012,26(6):543-546.
[7] 孙娟,黄焰,张玲,等.应用微电极芯片技术评价抗心房颤动药物对兔右心房快速起搏模型的影响[J].中国循环杂志,2014(8):639-642.
[8] 欧阳松,张晓雅,庄妤婷,等.鞣酸对大鼠心室肌场电位时限及兴奋传导速度影响的研究[J].中华心律失常学杂志,2016,20(5):430-434.
[9] 段亚琦,唐明,梁华敏.微电极矩阵研究小鼠胚胎心脏电生理活动[J].生理学报,2006,58(1):65-70.
[10] Sun J,Yan H,Wugeti N,et al.Microelectrode array measurement of potassium ion channel remodeling on the field action potential duration in rapid atrial pacing rabbits model [J].Int J Clin Exp Med,2015,8(1):249.
[11] Banyasz T,Szentandrassy N,Magyar J,et al.An emerging antiarrhythmic target:late sodium current[J].Curr Pharm Des,2015,21(8):1073-1090.
[12] Xue X,Guo D,Sun H,et al.Wenxin Keli suppresses ventricular triggered arrhythmias via selective inhibition of late sodium current[J].Pacing Clin Electrophysiol,2013,36(6):732-740.
[13] Minoura Y,Panama BK,Nesterenko VV,et al.Effect of Wenxin Keli and quinidine to suppress arrhythmo- genesis in an experimental model of Brugada syndrome[J].Heart Rhythm,2013,10(7):1054-1062.
[14] Kalifa J,Avula UM.The Chinese herb extract Wenxin Keli:atrial selectivity from the Far East[J].Heart Rhythm,2012,9(1):132-133.
[15] 郭继鸿.心脏的传导性及异常[J].临床心电学杂志,2006,15(6):450-461.