防风药材及其伪品的挥发油类成分气相指纹图谱研究
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摘要
目的:建立防风药材及其伪品的挥发油类成分的气相指纹图谱。方法:采用正己烷提取防风药材中的挥发油类成分,运用气相色谱法(GC)对33批防风药材的挥发油类成分进行分析,借助"中药色谱指纹图谱相似度评价系统2004A版"对所得色谱图进行分析。结果:各样品之间的相似度为0.030～0.990,说明防风及其伪品的挥发性成分具有较大差异。结论:所建立的防风药材及其伪品的指纹图谱在鉴别防风真伪上具有一定的参考性,同时可作为防风药材综合质量评价体系的补充。
Objective:To establish the gas chromatography fingerprint of volatile oils from Saposhnikoviae Radix and its adulterants.Methods:The volatile oils of Saposhnikoviae Radix were extracted by n-hexane,and the volatile oils of 33 batches of Saposhnikoviae Radix were analyzed by gC.The chromatogram was analyzed by similarity evaluation system 2004 A of chromatographic fingerprint of traditional Chinese medicine.Results:The similarity between the samples was 0.030～0.990,which indicated that the volatile components of Saposhnikoviae Radix and its adulterants had great difference.Conclusion:The fingerprint of Saposhnikoviae Radix and its counterfeit has some reference value in identifying the true and false of Saposhnikoviae Radix,and it can be used as a supplement to the comprehensive quality evaluation system of Saposhnikoviae Radix.
Objective:To establish the gas chromatography fingerprint of volatile oils from Saposhnikoviae Radix and its adulterants.Methods:The volatile oils of Saposhnikoviae Radix were extracted by n-hexane,and the volatile oils of 33 batches of Saposhnikoviae Radix were analyzed by gC.The chromatogram was analyzed by similarity evaluation system 2004 A of chromatographic fingerprint of traditional Chinese medicine.Results:The similarity between the samples was 0.030～0.990,which indicated that the volatile components of Saposhnikoviae Radix and its adulterants had great difference.Conclusion:The fingerprint of Saposhnikoviae Radix and its counterfeit has some reference value in identifying the true and false of Saposhnikoviae Radix,and it can be used as a supplement to the comprehensive quality evaluation system of Saposhnikoviae Radix.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015:149-150.
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[8]杨滨,黄璐琦,姚三桃,等.防风类药材高效液相色谱鉴别[J].中国药学杂志,1999(10):12-14.
[2]焦坤.高效液相色谱法同时测定炒防风中升麻素苷和5-O-甲基维斯阿米醇苷含量[J].中国药业,2018,27(1):35-37.
[3]杨晓琴,任红燕.高效液相色谱法同时测定防风中4种主要成分含量[J].中国药业,2017,26(20):37-40.
[4]项瑞.高效液相色谱法测定防风中色原酮的含量[J].首都医药,2014,21(18):75-77.
[5]李华.防风及其栽培品饮片质量控制与分析[J].医药导报,2010,29(10):1356-1357.
[6]李丽,刘质净,桂语歌,等.防风中的色原酮化合物的高效液相色谱与电喷雾质谱联用研究[J].时珍国医国药,2010,21(6):1310-1311.
[7]姜艳艳,刘斌,石任兵.高效液相色谱法测定防风色原酮部位中4种成分含量[J].北京中医药大学学报,2006(2):128-131.
[8]杨滨,黄璐琦,姚三桃,等.防风类药材高效液相色谱鉴别[J].中国药学杂志,1999(10):12-14.