HPLC-DAD同时测定益心通胶囊中6种活性成分的含量
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摘要
目的建立HPLC同时测定益心通胶囊中葛根素、龙胆苦苷、阿魏酸、毛蕊花糖苷、丹酚酸B和丹皮酚6种活性成分含量的方法。方法采用XSELECT~(TM) HSS C_(18)色谱柱,流动相为乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱;柱温:30℃;流速:1.0 mL·min-1;检测波长:阿魏酸、毛蕊花糖苷和丹酚酸B为220 nm,葛根素为250 nm,龙胆苦苷和丹皮酚为275 nm。结果葛根素、龙胆苦苷、阿魏酸、毛蕊花糖苷、丹酚酸B和丹皮酚的线性范围分别为7.56～113.4μg·mL~(-1)(r=0.999 8),4.36～65.4μg·mL~(-1)(r=0.999 7),1.18～17.7μg·mL~(-1)(r=0.999 3), 1.90～28.5μg·mL~(-1)(r=0.999 4), 4.48～67.2μg·mL~(-1)(r=0.999 3)和11.7～175.5μg·mL~(-1)(r=0.9997),平均加样回收率分别为98.7%,97.8%,97.6%,98.6%,99.0%,98.7%,RSD分别为1.0%,1.3%,1.4%,1.1%,1.2%,1.3%。结论本法专属性强,结果准确,重现性好,可用于益心通胶囊的质量控制。
OBJECTIVE To establish an HPLC-DAD method for determination of six bioactive components in Yixintong capsules,including puerarin,gentiopicroside,ferulic acid,acteoside,salvianolic acid B and paeonol.METHODS A stable HPLC method was established and the chromatography was accomplished on a XSELECT~(TM) HSS C_(18) column,the mobile phase consisted of methanol(A)-0.2%phosphoric acid(B)in a gradient mode.The flow rate was 1.0 mL·min~(-1) and the column temperature was set at 30℃.The detection wavelength was set at 220 nm for paeoniflorin,acteoside and salvianolic acid B,250 nm for puerarin,275 nm for gentiopicroside and paeonol,respectively.RESULTS The linear ranges of puerarin,gentiopicroside,paeoniflorin,acteoside,salvianolic acid B and paeonol were 7.56-113.4μg·mL~(-1)(r=0.999 8),4.36-65.4μg·mL~(-1)(r=0.999 7),1.18-17.7μg·m L~(-1)(r=0.999 3),1.90-28.5μg·m L~(-1)(r=0.999 4),4.48-67.2μg·m L~(-1)(r=0.999 3)and11.7-175.5μg·m L~(-1)(r=0.999 7),respectively.The average recoveries were 98.7%,97.8%,97.6%,98.6%,99.0%and 98.7%,RSD were 1.0%,1.3%,1.4%,1.1%,1.2%and 1.3%.CONCLUSION The method is specificity,accurate and reproducible,which can be used for the quality control of Yixintong capsules.
OBJECTIVE To establish an HPLC-DAD method for determination of six bioactive components in Yixintong capsules,including puerarin,gentiopicroside,ferulic acid,acteoside,salvianolic acid B and paeonol.METHODS A stable HPLC method was established and the chromatography was accomplished on a XSELECT~(TM) HSS C_(18) column,the mobile phase consisted of methanol(A)-0.2%phosphoric acid(B)in a gradient mode.The flow rate was 1.0 mL·min~(-1) and the column temperature was set at 30℃.The detection wavelength was set at 220 nm for paeoniflorin,acteoside and salvianolic acid B,250 nm for puerarin,275 nm for gentiopicroside and paeonol,respectively.RESULTS The linear ranges of puerarin,gentiopicroside,paeoniflorin,acteoside,salvianolic acid B and paeonol were 7.56-113.4μg·mL~(-1)(r=0.999 8),4.36-65.4μg·mL~(-1)(r=0.999 7),1.18-17.7μg·m L~(-1)(r=0.999 3),1.90-28.5μg·m L~(-1)(r=0.999 4),4.48-67.2μg·m L~(-1)(r=0.999 3)and11.7-175.5μg·m L~(-1)(r=0.999 7),respectively.The average recoveries were 98.7%,97.8%,97.6%,98.6%,99.0%and 98.7%,RSD were 1.0%,1.3%,1.4%,1.1%,1.2%and 1.3%.CONCLUSION The method is specificity,accurate and reproducible,which can be used for the quality control of Yixintong capsules.
引文
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[3]刘涛,才谦,付玉芹,等.中药龙胆的研究进展[J].辽宁中医杂志,2004,31(1):85-86.
[4]GUO Q F,WANG W,HAN W Q.Progress on pharmacological action of gentiopicroside[J].Guangdong Chem(广东化工),2014,41(17):121,128.
[5]李曦,张丽宏,王晓晓.当归化学成分及药理作用研究进展[J].中药材,2013,36(5):1023-1028.
[6]胡益勇,徐晓玉.阿魏酸的化学和药理研究进展[J].中成药,2006,28(2):253-255.
[7]ZHU Y,XU C.Pharmacological actions of radix rehmann praeparata and its active components:research advances[J].Aisa-Pacific Tradit Med(亚太传统医药),2011,7(11):173-175.
[8]夏庆华,路千里.熟地黄药理研究进展[J].江西中医学院学报,2008,20(6):96-97.
[9]马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014,25(7):663-665.
[10]王蓉,原永芳.丹酚酸B药理作用的研究概况[J].中医药导报,2011,17(4):130-132.
[11]张健萍,李连珍,赵红江,等.牡丹皮的化学成分、药理作用及临床应用研究概况[J].中华中医药杂志,2006,21(5):295-297.
[12]HU Y F,XU G B.Pharmacological activities of cortex moutan radicis and its main components of paeonol[J].Anhui Med J(安徽医药),2014,18(4):589-592.
[13]LU X G,CHEN X,LI J,et al.Simultaneous determination of tanshinol protocatechualdehyde,salvianolic acid B,ferulic acid and puerarin in Tongmai particles by HPLC[J].Chin JMod Appl Pharm(中国现代应用药学),2017,34(10):1373-1376.
[14]GAO S,BAI X,WEN L J,et al.Determination of six components in Shensu xuanfei wan by HPLC with wavelength switching combined gradient elution method[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(10):1300-1304.
[15]WANG Z Y,LI C Q,MU M J,et al.Simultaneous determination of the content of Danshensu,puerarin,dadzin and salvianolic acid B in Bushen Huoxue prescription by HPLC wavelength switching technology[J].Chin J Pharm Anal(药物分析杂志),2016,36(6):1020-1026.
[16]YU L R,HE X Y,HUANG Y R.Simultaneous determination of paeoniflorin,salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(5):561-564.
[17]WU W,ZHANG P Y,CHEN Y G,et al.Quality standard of Huangqi Baoxin mixture[J].Her Med(医药导报),2018,37(3):341-344.
[18]CAO Z Q,XU J W,LU L,et al.Study on quality standard of Fuzhengtongluo granules[J].Chin J New Drugs(中国新药杂志),2019,28(11):1372-1377.
[19]WANG D P,YAN X J.Simultaneous determination of loganin acid and gentiopicroside in Tongfengding tablets by HPLC[J].Mod Chin Med(中国现代中药),2014,16(12):1024-1026.
[20]WU X D,SU C,GUAN X Y,et al.HPLC simultaneous determination of gallic acid,tanshinol,protocatechuic aldehyde and paeoniflorin in Xinning Tablets[J].Pharm Today(今日药学),2018,28(9):595-598.