MAG2复合体MAG2和MIP3二亚基缺失对植物生长发育的影响
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摘要
真核细胞中,各细胞器之间的物质交流主要是通过膜泡运输完成的。膜泡运输依赖多种跨膜蛋白和可溶性蛋白的协同作用来完成。我们前期研究发现的拟南芥MAG2是在高尔基体与内质网之间的膜泡运输过程中起作用的拴留因子,MAG2与MIP1、MIP2和MIP3形成拴留复合体,对种子储藏蛋白从内质网的运离起重要的调控作用。为了深入了解MAG2参与的膜泡运输途径的分子机制,我们制作了mag2-1xmip3-1双重突变体。通过一系列观察分析发现,当MAG2和MIP3同时缺失,不仅种子储藏蛋白质的运输受到严重阻碍,植物的生长发育也受到严重影响,对环境条件的变化更加敏感。研究结果表明,MAG2复合体不仅调控蛋白质的运输,还对植物的生长发育有重要调控作用。
In eukaryotic cells, material transport between organelles is completed mainly by vesicle trafficking.Vesicle transport dependents on the synergistic function of a variety of transmembrane proteins and soluble proteins. Our previous study found that Arabidopsis MAG2 is a tethering factor functions between the ER-Golgi.MAG2, MIP1, MIP2 and MIP3 form a tethering complex that plays an important role on exit of seed storage proteins from the ER. To clarify molecular mechanism underlying MAG2-involved transport pathways, we made mag2-1xmip3-1 double mutant. Through a series of observation and analyses, we found that simultaneous d epletion of MAG2 and MIP3 not only block exit of the seed storage proteins transport, but also affect plant growth and development. The mutant plant is more sensitive to environmental stress. The results indicate that in addition to regulation of transport of proteins, MAG2 complex is also involved in the growth and development of plants.
In eukaryotic cells, material transport between organelles is completed mainly by vesicle trafficking.Vesicle transport dependents on the synergistic function of a variety of transmembrane proteins and soluble proteins. Our previous study found that Arabidopsis MAG2 is a tethering factor functions between the ER-Golgi.MAG2, MIP1, MIP2 and MIP3 form a tethering complex that plays an important role on exit of seed storage proteins from the ER. To clarify molecular mechanism underlying MAG2-involved transport pathways, we made mag2-1xmip3-1 double mutant. Through a series of observation and analyses, we found that simultaneous d epletion of MAG2 and MIP3 not only block exit of the seed storage proteins transport, but also affect plant growth and development. The mutant plant is more sensitive to environmental stress. The results indicate that in addition to regulation of transport of proteins, MAG2 complex is also involved in the growth and development of plants.
引文
Andag U.,Neumann T.,and Schmitt H.D.,2001,The coatomerinteracting protein Dsl1p is required for golgi-to-endoplasmic reticulum retrieval in yeast,J.Biol.Chem.,276(42):39150-39160
Aoki T.,Ichimura S.,Itoh A.,Kuramoto M.,Shinkawa T.,IsobeT.,and Tagaya M.,2009,Identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18complex implicated in Golgi-to-endoplasmic reticulum retrograde transport,Mol.Biol.Cell,20(11):2639-2649
Bracher A.,and Weissenhorn W.,2001,Crystal structures of neuronal squid Sec1 implicate inter-domain hinge movement inthe release of t-SNAREs,J.Mol.Biol.,306(1):7-13
Bracher A.,and Weissenhorn W.,2002,Structural basis for theGolgi membrane recruitment of Sly1p by Sed5p,EMBO J.,21(22):6114-6124
Cai H.,Reinisch K.,and Ferro-Novick S.,2007,Coats,tethers,Rabs,and SNAREs work together to mediate the intracellular destination of a transport vesicle,Dev.Cell,12(5):671-682
Civril F.,Wehenkel A.,Giorgi F.M.,Santaguida S.,Di Fonzo A.,Grigorean G.,Ciccarelli F.D.,and Musacchio A.,2010,Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery,Structure,18(5):616-626
Cosson P.,Schrder-Khne S.,Sweet D.S.,Dmollire C.,Hennecke S.,Frigerio G.,and Letourneur F.,1997,TheSec20/Tip20p complex is involved in ER retrieval of dilysine-tagged proteins,Eur.J.Cell Biol.,73(2):93-97
Duden R.,Griffiths G.,Frank R.,Argos P.,and Kreis T.E.,1991,Beta-COP,a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex,shows homology to beta-adaptin,Cell,64(3):649-665
Harrison-Lavoie K.J.,Lewis V.A.,Hynes G.M.,Collison K.S.,Nutland E.,and Willison K.R.,1993,A 102 kD a subunit ofa Golgi-associated particle has homology to beta subunits oftrimeric G proteins,EMBO J.,12(7):2847-2853
Ito T.,Chiba T.,Ozawa R.,Yoshida M.,Hattori M.,and SakakiY.,2001,A comprehensive two-hybrid analysis to explorethe yeast protein interactome,Proc.Natl.Acad.Sci.USA,98(8):4569-4574
Li L.,Shimada T.,Takahashi H.,Koumoto Y.,Shirakawa M.,Takagi J.,Zhao X.,Tu B.,Jin H.,Shen Z.,Han B.,Jia M.,Kondo M.,Nishimura M.,and Hara-Nishimura I.,2013,MAG2 and three MAG2-INTERACTING PROTEINs forman ER-localized complex to facilitate storage protein transport in Arabidopsis thaliana,Plant J.,76(5):781-791
Li L.,Shimada T.,Takahashi H.,Ueda H.,Fukao Y.,Kondo M.,Nishimura M.,and Hara-Nishimura I.,2006,MAIGO2 isinvolved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana,Plant Cell,18(12):3535-3547
Mizuno-Yamasaki E.,Rivera-Molina F.,and Novick P.,2012,GTPase networks in membrane traffic,Annu.Rev.Biochem.,81:637-659
Mnaimneh S.,Davierwala A.P.,Haynes J.,Moffat J.,Peng W.T.,Zhang W.,Yang X.,Pootoolal J.,Chua G.,Lopez A.,Trochesset M.,Morse D.,Krogan N.J.,Hiley S.L.,Li Z.,Morris Q.,Grigull J.,Mitsakakis N.,Roberts C.J.,Greenblatt J.F.,Boone C.,Kaiser C.A.,Andrews B.J.,and HughesT.R.,2004,Exploration of essential gene functions via titratable promoter alleles,Cell,118(1):31-44
Reilly B.A.,Kraynack B.A.,VanR heenen S.M.,and Waters M.G.,2001,Golgi-to-endoplasmic reticulum(ER)retrograde traffic in yeast requires Dsl1p,a component of the ER target sitethat interacts with a COPI coat subunit,Mol.Biol.Cell,12(12):3783-3796
Seemann J.,Jokitalo E.J.,and Warren G.,2000,The role of thetethering proteins p115 and GM130 in transport through theGolgi apparatus in vivo,Mol.Biol.Cell,11(2):635-645
Serafini T.,Stenbeck G.,Brecht A.,Lottspeich F.,Orci L.,Rothman J.E.,and Wieland F.T.,1991,A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to theclathrin-coated vesicle coat protein beta-adaptin,Nature,349(6306):215-220
Spang A.,2012,The DSL1 complex:the smallest but not theleast CATCHR,Traffic,13(7):908-913
Stenbeck G.,Harter C.,Brecht A.,Herrmann D.,Lottspeich F.,Orci L.,and Wieland F.T.,1993,beta'-COP,a novel subunitof coatomer,EMBO J.,12(7):2841-2845
Sweet D.J.,and Pelham H.R.,1993,The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kD a cytoplasmic proteinthat interacts with the cytoplasmic domain of Sec20p,EMBO J.,12(7):2831-2840
Vanrheenen S.M.,Reilly B.A.,Chamberlain S.J.,and Waters M.G.,2001,Dsl1p,an essential protein required for membranetraffic at the endoplasmic reticulum/Golgi interface in yeast,Traffic,2(3):212-231
Zhang Z.Y.,Yang X.L.,Zhao X.N.,Zhang H.L.,Shen Z.,and LiL.X.,2015,Effects of SYP7 family genes on plant growthand development,Fenzi Zhiwu Yuzhong(Molecular PlantBreeding),13(4):711-716(张忠阳,杨孝丽,赵潇男,张海龙,申哲,李立新,2015,SYP7家族基因对植物生长发育的影响,分子植物育种,13(4):711-716)
Aoki T.,Ichimura S.,Itoh A.,Kuramoto M.,Shinkawa T.,IsobeT.,and Tagaya M.,2009,Identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18complex implicated in Golgi-to-endoplasmic reticulum retrograde transport,Mol.Biol.Cell,20(11):2639-2649
Bracher A.,and Weissenhorn W.,2001,Crystal structures of neuronal squid Sec1 implicate inter-domain hinge movement inthe release of t-SNAREs,J.Mol.Biol.,306(1):7-13
Bracher A.,and Weissenhorn W.,2002,Structural basis for theGolgi membrane recruitment of Sly1p by Sed5p,EMBO J.,21(22):6114-6124
Cai H.,Reinisch K.,and Ferro-Novick S.,2007,Coats,tethers,Rabs,and SNAREs work together to mediate the intracellular destination of a transport vesicle,Dev.Cell,12(5):671-682
Civril F.,Wehenkel A.,Giorgi F.M.,Santaguida S.,Di Fonzo A.,Grigorean G.,Ciccarelli F.D.,and Musacchio A.,2010,Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery,Structure,18(5):616-626
Cosson P.,Schrder-Khne S.,Sweet D.S.,Dmollire C.,Hennecke S.,Frigerio G.,and Letourneur F.,1997,TheSec20/Tip20p complex is involved in ER retrieval of dilysine-tagged proteins,Eur.J.Cell Biol.,73(2):93-97
Duden R.,Griffiths G.,Frank R.,Argos P.,and Kreis T.E.,1991,Beta-COP,a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex,shows homology to beta-adaptin,Cell,64(3):649-665
Harrison-Lavoie K.J.,Lewis V.A.,Hynes G.M.,Collison K.S.,Nutland E.,and Willison K.R.,1993,A 102 kD a subunit ofa Golgi-associated particle has homology to beta subunits oftrimeric G proteins,EMBO J.,12(7):2847-2853
Ito T.,Chiba T.,Ozawa R.,Yoshida M.,Hattori M.,and SakakiY.,2001,A comprehensive two-hybrid analysis to explorethe yeast protein interactome,Proc.Natl.Acad.Sci.USA,98(8):4569-4574
Li L.,Shimada T.,Takahashi H.,Koumoto Y.,Shirakawa M.,Takagi J.,Zhao X.,Tu B.,Jin H.,Shen Z.,Han B.,Jia M.,Kondo M.,Nishimura M.,and Hara-Nishimura I.,2013,MAG2 and three MAG2-INTERACTING PROTEINs forman ER-localized complex to facilitate storage protein transport in Arabidopsis thaliana,Plant J.,76(5):781-791
Li L.,Shimada T.,Takahashi H.,Ueda H.,Fukao Y.,Kondo M.,Nishimura M.,and Hara-Nishimura I.,2006,MAIGO2 isinvolved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana,Plant Cell,18(12):3535-3547
Mizuno-Yamasaki E.,Rivera-Molina F.,and Novick P.,2012,GTPase networks in membrane traffic,Annu.Rev.Biochem.,81:637-659
Mnaimneh S.,Davierwala A.P.,Haynes J.,Moffat J.,Peng W.T.,Zhang W.,Yang X.,Pootoolal J.,Chua G.,Lopez A.,Trochesset M.,Morse D.,Krogan N.J.,Hiley S.L.,Li Z.,Morris Q.,Grigull J.,Mitsakakis N.,Roberts C.J.,Greenblatt J.F.,Boone C.,Kaiser C.A.,Andrews B.J.,and HughesT.R.,2004,Exploration of essential gene functions via titratable promoter alleles,Cell,118(1):31-44
Reilly B.A.,Kraynack B.A.,VanR heenen S.M.,and Waters M.G.,2001,Golgi-to-endoplasmic reticulum(ER)retrograde traffic in yeast requires Dsl1p,a component of the ER target sitethat interacts with a COPI coat subunit,Mol.Biol.Cell,12(12):3783-3796
Seemann J.,Jokitalo E.J.,and Warren G.,2000,The role of thetethering proteins p115 and GM130 in transport through theGolgi apparatus in vivo,Mol.Biol.Cell,11(2):635-645
Serafini T.,Stenbeck G.,Brecht A.,Lottspeich F.,Orci L.,Rothman J.E.,and Wieland F.T.,1991,A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to theclathrin-coated vesicle coat protein beta-adaptin,Nature,349(6306):215-220
Spang A.,2012,The DSL1 complex:the smallest but not theleast CATCHR,Traffic,13(7):908-913
Stenbeck G.,Harter C.,Brecht A.,Herrmann D.,Lottspeich F.,Orci L.,and Wieland F.T.,1993,beta'-COP,a novel subunitof coatomer,EMBO J.,12(7):2841-2845
Sweet D.J.,and Pelham H.R.,1993,The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kD a cytoplasmic proteinthat interacts with the cytoplasmic domain of Sec20p,EMBO J.,12(7):2831-2840
Vanrheenen S.M.,Reilly B.A.,Chamberlain S.J.,and Waters M.G.,2001,Dsl1p,an essential protein required for membranetraffic at the endoplasmic reticulum/Golgi interface in yeast,Traffic,2(3):212-231
Zhang Z.Y.,Yang X.L.,Zhao X.N.,Zhang H.L.,Shen Z.,and LiL.X.,2015,Effects of SYP7 family genes on plant growthand development,Fenzi Zhiwu Yuzhong(Molecular PlantBreeding),13(4):711-716(张忠阳,杨孝丽,赵潇男,张海龙,申哲,李立新,2015,SYP7家族基因对植物生长发育的影响,分子植物育种,13(4):711-716)