SYP7家族基因对植物生长发育的影响
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摘要
植物在进化过程中获得特有的膜泡运输系统。其中,SYP7家族(包括SYP71,SYP72和SYP73)在植物细胞中参与膜泡运输和其他一些生命活动,如SYP71可能参与了植物细胞有丝分裂细胞板的形成,并调控豆科植物Lotus Japonicas的固氮作用;SYP72在高尔基体到内质网的逆向运输载体COPI小泡融合到内质网上起重要作用。本研究通过对SYP7家族中各基因的T-DNA插入突变体和过表达植株中表型分析,揭示了SYP7家族表达量的变化对拟南芥发育早期根的生长的影响。
Plants established their specific vesicle trafficking system during evolution. SYP7 family(including SYP71, SYP72 and SYP73) is involved in vesicle trafficking and other events in plant cells. For example, SYP71 is involved in cell plate formation during cytokinesis. SYP71 also regulates nitrogen fixation activity in Lotus Japonicas. SYP72 functions on retrograde transport from the Golgi apparatus to the ER when COPI vesicle fused to the ER membrane. In this study, we analyzed phenotypes of T-DNA insertion mutants and over-expression transgenic plants of SYP7 genes. The results indicated that the changes of expression level of the SYP7 genes affect Arabidopsis root growth in early development stage.
Plants established their specific vesicle trafficking system during evolution. SYP7 family(including SYP71, SYP72 and SYP73) is involved in vesicle trafficking and other events in plant cells. For example, SYP71 is involved in cell plate formation during cytokinesis. SYP71 also regulates nitrogen fixation activity in Lotus Japonicas. SYP72 functions on retrograde transport from the Golgi apparatus to the ER when COPI vesicle fused to the ER membrane. In this study, we analyzed phenotypes of T-DNA insertion mutants and over-expression transgenic plants of SYP7 genes. The results indicated that the changes of expression level of the SYP7 genes affect Arabidopsis root growth in early development stage.
引文
Bonifacino J.S.,and Glick B.S.,2004,The mechanisms of vesiclbudding and fusion,Cell,116(2):153-166
Box M.S.,Coustham V.,Dean C.,and Mylne J.S.,2011,ProtocolA simple phenol-based method for 96-well extraction of highquality RNA from Arabidopsis,Plant Methods,7:7
Cai H.Q.,Reinisch K.,and Ferro-Novick S.,2007,Coats,tethersRabs,and SNAREs work together to mediate the intracelluladestination of a transport vesicle,Dev.Cell,12(5):671-682
El Kasmi F.,Krause C.,Hiller U.,Stierhof Y.D.,Mayer U.,ConneL.,Kong L.,Reichardt I.,Sanderfoot A.A.,and Jurgens G2013,SNARE complexes of different composition jointlmediate membrane fusion in Arabidopsis cytokinesis,MolBiol.Cell,24(10):1593-1601
Hakoyama T.,Oi R.,Hazuma K.,Suga E.,Adachi Y.,KobayashM.,Akai R.,Sato S.,Fukai E.,Tabata S.,Shibata S.,Wu G.JHase Y.,Tanaka A.,Kawaguchi M.,Kouchi H.,Umehara Yand Suganuma N.,2012,The SNARE protein SYP71 expressed in vascular tissues is involved in symbiotic nitrogenfixation in Lotus japonicus nodules,Plant Physiol.,160(2)897-905
Lerich A.,Hillmer S.,Langhans M.,Scheuring D.,van Bentum P.and Robinson D.G.,2012,ER Import Sites and Their relationship to ER exit sites:a new model for bidirectionaER-golgi transport in higher plants,Front Plant Sci.,3:143
Lipka V.,Kwon C.,and Panstruga R.,2007,SNARE-ware:throle of SNARE-domain proteins in plant biology,Annu.RevCell Dev.Biol.,23:147-174
Shimada T.,Fuji K.,Tamura K.,Kondo M.,Nishimura M.,anHara-Nishimura I.,2003,Vacuolar sorting receptor for seedstorage proteins in Arabidopsis thaliana,Proc.Natl.AcadSci.USA,100(26):16095-16100
Suwastika I.N.,Uemura T.,Shiina T.,Sato M.H.,and Takeyasu K2008,SYP71,a plant-specific Qc-SNARE protein,revealdual localization to the plasma membrane and the endoplasmic reticulum in Arabidopsis,Cell Struct.Funct.,33(2)185-192
Box M.S.,Coustham V.,Dean C.,and Mylne J.S.,2011,ProtocolA simple phenol-based method for 96-well extraction of highquality RNA from Arabidopsis,Plant Methods,7:7
Cai H.Q.,Reinisch K.,and Ferro-Novick S.,2007,Coats,tethersRabs,and SNAREs work together to mediate the intracelluladestination of a transport vesicle,Dev.Cell,12(5):671-682
El Kasmi F.,Krause C.,Hiller U.,Stierhof Y.D.,Mayer U.,ConneL.,Kong L.,Reichardt I.,Sanderfoot A.A.,and Jurgens G2013,SNARE complexes of different composition jointlmediate membrane fusion in Arabidopsis cytokinesis,MolBiol.Cell,24(10):1593-1601
Hakoyama T.,Oi R.,Hazuma K.,Suga E.,Adachi Y.,KobayashM.,Akai R.,Sato S.,Fukai E.,Tabata S.,Shibata S.,Wu G.JHase Y.,Tanaka A.,Kawaguchi M.,Kouchi H.,Umehara Yand Suganuma N.,2012,The SNARE protein SYP71 expressed in vascular tissues is involved in symbiotic nitrogenfixation in Lotus japonicus nodules,Plant Physiol.,160(2)897-905
Lerich A.,Hillmer S.,Langhans M.,Scheuring D.,van Bentum P.and Robinson D.G.,2012,ER Import Sites and Their relationship to ER exit sites:a new model for bidirectionaER-golgi transport in higher plants,Front Plant Sci.,3:143
Lipka V.,Kwon C.,and Panstruga R.,2007,SNARE-ware:throle of SNARE-domain proteins in plant biology,Annu.RevCell Dev.Biol.,23:147-174
Shimada T.,Fuji K.,Tamura K.,Kondo M.,Nishimura M.,anHara-Nishimura I.,2003,Vacuolar sorting receptor for seedstorage proteins in Arabidopsis thaliana,Proc.Natl.AcadSci.USA,100(26):16095-16100
Suwastika I.N.,Uemura T.,Shiina T.,Sato M.H.,and Takeyasu K2008,SYP71,a plant-specific Qc-SNARE protein,revealdual localization to the plasma membrane and the endoplasmic reticulum in Arabidopsis,Cell Struct.Funct.,33(2)185-192