某羊场和田羊与策勒黑羊无浆体季节性感染PCR检测
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摘要
为了解和田羊和策勒黑羊无浆体季节动态感染情况,分别于2015年冬至、2016年春分、夏至和秋分,在策勒县某牧业合作社采集和田羊和策勒黑羊血液样本共120份,应用PCR对无浆体的MSP4基因和16SrRNA基因进行扩增。结果发现,检测绵羊的无浆体总阳性率为99.2%(119/120),以绵羊无浆体(Anaplasma ovis)和嗜吞噬无浆体(A.phagocytophilum)混合感染为主,占阳性样本比例为61.3%(73/119),A.ovis和A.phagocytophilum占阳性样本比例分别为31.1%(37/119)和7.6%(9/119)。在不同季节,和田羊和策勒黑羊无浆体均普遍感染,差异不显著(P>0.05)。结果显示,新疆策勒县某羊场绵羊的无浆体病全年均可流行,应引起人们重视。
To understand the prevalence of Anaplasmain Hotan sheep and Qika black sheep in various seasons,120 blood samples of Hotan sheep and Qika black sheep were collected from a sheep farm in Qika county in the Winter Solstice in 2015,the Spring Equinox,the Summer Solstice and the Autumn Equinox in2016,respectively.All samples were examined by PCR based on the MSP4 and 16SrRNA genes of Anaplasma.The overall positive rate of Anaplamain sheep was 99.2%(119/120).Co-infections with Anaplasma ovis and A.phagocytophilum were predominant and the proportion of them in positive samples was61.3%(73/119).The proportion of A.ovis and A.phagocytophilumin positive samples was 31.1%(37/119)and 7.6%(9/119),respectively.The Anaplasmainfections in Hotan sheep and Qika black sheep were common in various seasons,and the difference was not significant(P>0.05).The result showed that Anaplasmainfections in sheep can occur throughout the year in Qika county Xinjiang,which should be noticed.
To understand the prevalence of Anaplasmain Hotan sheep and Qika black sheep in various seasons,120 blood samples of Hotan sheep and Qika black sheep were collected from a sheep farm in Qika county in the Winter Solstice in 2015,the Spring Equinox,the Summer Solstice and the Autumn Equinox in2016,respectively.All samples were examined by PCR based on the MSP4 and 16SrRNA genes of Anaplasma.The overall positive rate of Anaplamain sheep was 99.2%(119/120).Co-infections with Anaplasma ovis and A.phagocytophilum were predominant and the proportion of them in positive samples was61.3%(73/119).The proportion of A.ovis and A.phagocytophilumin positive samples was 31.1%(37/119)and 7.6%(9/119),respectively.The Anaplasmainfections in Hotan sheep and Qika black sheep were common in various seasons,and the difference was not significant(P>0.05).The result showed that Anaplasmainfections in sheep can occur throughout the year in Qika county Xinjiang,which should be noticed.
引文
[1]宝福凯,柳爱华,马海滨.人类无浆体病的病原学和流行病学研究进展[J].热带医学杂志,2008,8(11):1193-1195.
[2] Hornok S,Elek V,de la Fuente J,et al.First serological and molecular evidence on the endemicity of Anaplasma ovis and A.marginalein Hungary[J].Vet Microbiol,2007,122(3-4):316-322.
[3]孙明,刘志杰,马米玲,等.甘肃省景泰县羊无浆体病的诊断[J].动物医学进展,2012,33(4):114-117.
[4]吕文顺,吕文祥,张其才,等.山羊边虫病的研究-病原鉴定和临床症状的观察[J].中国兽医科技,1987,2(3):5-8.
[5] Liu Z,Ma M,Wang Z,et al.Molecular survey and genetic identification of Anaplasma species in goats from central and southern China[J].Appl Environ Microbiol,2012,78(2):464-470.
[6] Yang J,Li Y,Liu Z,et al.Molecular detection and characterization of Anaplasma spp.in sheep and cattle from Xinjiang,northwest China[J].Parasit Vectors,2015,8:108.
[7]张文静,张志敏,崔艳艳,等.我国部分地区奶山羊无浆体感染情况的调查[J].动物医学进展,2015,36(9):123-127.
[8]孙彩琴,刘建枝,黄磊,等.西藏当雄县无浆体分子流行病学调查[J].动物医学进展,2012,33(10):9-13.
[9]迟庆安,刘志杰,李有全,等.中国南方七省份牛无浆体病的流行病学调查[J].中国兽医科学,2014,44(1):98-103.
[10]季志东,胡天佑,宋步刚,等.山羊边虫病的研究——流行病学调查及治疗[J].中国兽医科技,1989(5):8-9.
[11] Yang J,Han R,Niu Q,et al.Occurrence of four Anaplasma species with veterinary and public health significance in sheep,northwestern China[J].Ticks Tick Borne Dis,2018,9(1):82-85.
[12] Ismail N,McBride J W.Tick-borne emerging infections:Ehrlichiosis and Anaplasmosis[J].Clin Lab Med,2017,37(2):317-340.
[13] Bown K J,Lambin X,Telford G R,et al.Relative importance of Ixodes ricinus and Ixodes trianguliceps as vectors for Anaplasma phagocytophilum and Babesia microti in field vole(Microtus agrestis)populations[J].Appl Environ Microbiol,2008,74(23):7118-7125.
[14] Baldridge G D,Scoles G A,Burkhardt N Y,et al.Transovarial transmission of francisella-like endosymbionts and Anaplasma phagocytophilumvariants in Dermacentor albipictus(Acari:Ixodidae)[J].J Med Entomol,2009,46(3):625-632.
[15] Belkahia H,Ben Said M,El Mabrouk N,et al.Seasonal dynamics,spatial distribution and genetic analysis of Anaplasma species infecting small ruminants from Northern Tunisia[J].Infect Genet Evol,2017,54:66-73.
[2] Hornok S,Elek V,de la Fuente J,et al.First serological and molecular evidence on the endemicity of Anaplasma ovis and A.marginalein Hungary[J].Vet Microbiol,2007,122(3-4):316-322.
[3]孙明,刘志杰,马米玲,等.甘肃省景泰县羊无浆体病的诊断[J].动物医学进展,2012,33(4):114-117.
[4]吕文顺,吕文祥,张其才,等.山羊边虫病的研究-病原鉴定和临床症状的观察[J].中国兽医科技,1987,2(3):5-8.
[5] Liu Z,Ma M,Wang Z,et al.Molecular survey and genetic identification of Anaplasma species in goats from central and southern China[J].Appl Environ Microbiol,2012,78(2):464-470.
[6] Yang J,Li Y,Liu Z,et al.Molecular detection and characterization of Anaplasma spp.in sheep and cattle from Xinjiang,northwest China[J].Parasit Vectors,2015,8:108.
[7]张文静,张志敏,崔艳艳,等.我国部分地区奶山羊无浆体感染情况的调查[J].动物医学进展,2015,36(9):123-127.
[8]孙彩琴,刘建枝,黄磊,等.西藏当雄县无浆体分子流行病学调查[J].动物医学进展,2012,33(10):9-13.
[9]迟庆安,刘志杰,李有全,等.中国南方七省份牛无浆体病的流行病学调查[J].中国兽医科学,2014,44(1):98-103.
[10]季志东,胡天佑,宋步刚,等.山羊边虫病的研究——流行病学调查及治疗[J].中国兽医科技,1989(5):8-9.
[11] Yang J,Han R,Niu Q,et al.Occurrence of four Anaplasma species with veterinary and public health significance in sheep,northwestern China[J].Ticks Tick Borne Dis,2018,9(1):82-85.
[12] Ismail N,McBride J W.Tick-borne emerging infections:Ehrlichiosis and Anaplasmosis[J].Clin Lab Med,2017,37(2):317-340.
[13] Bown K J,Lambin X,Telford G R,et al.Relative importance of Ixodes ricinus and Ixodes trianguliceps as vectors for Anaplasma phagocytophilum and Babesia microti in field vole(Microtus agrestis)populations[J].Appl Environ Microbiol,2008,74(23):7118-7125.
[14] Baldridge G D,Scoles G A,Burkhardt N Y,et al.Transovarial transmission of francisella-like endosymbionts and Anaplasma phagocytophilumvariants in Dermacentor albipictus(Acari:Ixodidae)[J].J Med Entomol,2009,46(3):625-632.
[15] Belkahia H,Ben Said M,El Mabrouk N,et al.Seasonal dynamics,spatial distribution and genetic analysis of Anaplasma species infecting small ruminants from Northern Tunisia[J].Infect Genet Evol,2017,54:66-73.