莲原花青素与金属离子相互作用对抗氧化的影响
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摘要
采用DPPH·测试法研究莲原花青素(LSPC)与金属离子反应前、后对清除DPPH·能力的影响以及对抑制脂肪氧化和脂肪氧合酶酶活能力的影响。结果表明:LSPC与金属离子反应后清除DPPH·的能力较LSPC强。金属离子与LSPC协同作用比单独使用LSPC的抑制脂肪氧化能力强。金属离子与LSPC的配合物具有强的抗氧化能力。低浓度的金属离子与LSPC有协同抑制脂肪氧合酶酶活的能力。金属离子与LSPC反应后对脂肪氧合酶酶活的抑制能力较LSPC明显提高。
The DPPH· test was used to study the effeacts of anthocyanin and metal ions on DPPH· scavenging ability and the inhibition of lipoxygenase and lipoxygenase activity. The results showed that the ability of LSPC to remove DPPH· after reaction with metal ions was stronger than LSPC. The interaction between metal ions and LSPC was stronger than that of LSPC alone, and the complexes of metal ions with LSPC had stronger antioxidant capacity. Low concentration of metal ions and LSPC have synergistic inhibition of lipoxygenase enzyme activity, metal ions and LSPC response to lipoxygenase enzyme activity inhibition than LSPC has significantly improved.
The DPPH· test was used to study the effeacts of anthocyanin and metal ions on DPPH· scavenging ability and the inhibition of lipoxygenase and lipoxygenase activity. The results showed that the ability of LSPC to remove DPPH· after reaction with metal ions was stronger than LSPC. The interaction between metal ions and LSPC was stronger than that of LSPC alone, and the complexes of metal ions with LSPC had stronger antioxidant capacity. Low concentration of metal ions and LSPC have synergistic inhibition of lipoxygenase enzyme activity, metal ions and LSPC response to lipoxygenase enzyme activity inhibition than LSPC has significantly improved.
引文
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[13] INOUE M B, INOUE M, Fernando Q, et a1. Potentiometric and H1NMR studies of complexation of Al(Ⅲ)with(-)-epigallocatechin gallate, a major active constituent of green tea[J]. Journal of Inorganic Biochemistey, 2002, 88(1):7-13.
[14] KOSTYUK V A, POTAPOVICH A I, VLADYKOVSKAYA E N, et al. Influence of metal ions on flavonoid protection against asbestos-induceded cell injury[J]. Arch Bioehem BioPHys, 2001, 385(1):129-137.
[15]钟珍萍.大豆脂氧合酶的研究概况[J].福建农业科技, 1992,(5):21-22.
[2]凌智群,谢笔钧.莲房原花青素及其生物、药理活性研究[D].武汉:华中农业大学, 2001.
[3]沈生荣,赵玉芳,赵保路.铁存在下儿茶素的抗自由基效应[J].茶叶科学, 1997(s1):40-44.
[4]王辉.大豆脂肪氧合酶纯化及同工酶特性比较研究[D].上海:上海海洋大学, 2008.
[5]田其英,尹贵中,华欲飞.大豆脂肪氧合酶同工酶活性的影响因素研究[J].食品工业科技, 2008(1):156-159.
[6] FERNANDEZ M T, MIRA M L, FLORENCIO M H, et al. Iron and copper chelation by flavonoids:an electrospray mass spectrometry study[J]. J Inorg Biochem, 2002, 92(2):105-111.
[7] OHYOSHI E, SAKATA T, KURIHARA M. Complexation of al-uminium with(-)-epigallocathechin gallate studied by spectrophotometry[J]. Journal of Inorganic Biochemistry, 1999, 73(1/2):31-34.
[8] VAN ACKER S A B E, VAN BALEN G P, VAN DEN BERG D J, et a1. Influence of ironchelation on the antioxidantactivity of flavonoids[J]. Biochemical Pharmacology, 1998, 568:935-943.
[9]张红雨.黄酮类抗氧化剂结构活性关系的理论解释[J].中国科学, 1999, 29(1):91-96.
[10] GUST J. SUWALSKI J. Relationship between radical scavenging effects and anticorrosive properties of polypHenols[J]. Corrosion, 1995, 51(1):37-44.
[11] SAKANO K, MIZUTANI M, MURATA M, et al.Procyanidin B2 has anti-and pro-oxidant effects on metal-mediated DNA damage[J]. Free Radical Biology&Medicine, 2005, 39(8?):1041-1049.
[12]陈书婷.脂肪氧合酶的分离纯化及其催化多不饱和脂肪酸的研究[D].无锡:江南大学, 2011.
[13] INOUE M B, INOUE M, Fernando Q, et a1. Potentiometric and H1NMR studies of complexation of Al(Ⅲ)with(-)-epigallocatechin gallate, a major active constituent of green tea[J]. Journal of Inorganic Biochemistey, 2002, 88(1):7-13.
[14] KOSTYUK V A, POTAPOVICH A I, VLADYKOVSKAYA E N, et al. Influence of metal ions on flavonoid protection against asbestos-induceded cell injury[J]. Arch Bioehem BioPHys, 2001, 385(1):129-137.
[15]钟珍萍.大豆脂氧合酶的研究概况[J].福建农业科技, 1992,(5):21-22.