温度突变对毛蚶不同组织抗氧化酶活性的影响
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摘要
为探究温度突变对毛蚶鳃、外套膜和肝胰腺组织中抗氧化酶活性的影响,将毛蚶由11℃(对照组)分别转移至14、17、20、23℃4个温度条件下,在转移后的0、3、6、12、24、48、96 h进行取样,测定毛蚶3种组织中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性的变化情况。试验结果表明,毛蚶鳃、外套膜和肝胰腺中的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性在试验期间均呈先升后降的趋势;除20℃和14℃温度组外套膜和肝胰腺中的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性,在6 h到达最大值并显著高于对照组(P<0.05)外,其余温度组各组织中3种抗氧化酶活性,均在3 h时达到最大值并显著高于对照组(P<0.05),24 h后各组织抗氧化酶活性均恢复至对照组水平。毛蚶3种组织中的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性在不同温度下均以肝胰腺最高,外套膜次之,鳃最低。本研究探索了温度突变对毛蚶的存活及抗氧化酶活性的影响,明确了毛蚶对温度突变的自身调节过程,为毛蚶养殖提供了科学依据。
In order to understand the effect of sudden temperature change on the antioxidant enzyme activity in different organs of ark shell Scapharca subcrenata, ark shell living in 11 ℃(control group) were transferred directly into four different temperature conditions of 14 ℃, 17 ℃, 20 ℃ and 23 ℃. Activities of superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GSH-PX) were determined in the organs of gill, mantle and hepatopancreas of ark shell sampled 0, 3, 6, 12, 24, 48 and 96 hours after the transferring. The results showed that with the rising of the temperature and the increase in treatment time, activities of SOD, CAT and GSH-PX in all the organs were increased at first and decreased afterwards. The maximal activities of of SOD, CAT and GSH-PX were found to be signifantly higher in mantle and hepatopancreas in 14 ℃ group and 20 ℃ group after 6 hours of treatment than that in the control(P<0.05). For the rest temperature groups the maximal activities of the three antioxidant enzymes were observed after 3 hours, significantly higher than that in the control(P<0.05). After 24 hours, activities of antioxidant enzyme of all groups recovered to the level in the control. In all temperature groups, the highest activity level of SOD, CAT and GSH-PX appeared in hepatopancreas, followed by the mantle and the minimal values in gill.
In order to understand the effect of sudden temperature change on the antioxidant enzyme activity in different organs of ark shell Scapharca subcrenata, ark shell living in 11 ℃(control group) were transferred directly into four different temperature conditions of 14 ℃, 17 ℃, 20 ℃ and 23 ℃. Activities of superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GSH-PX) were determined in the organs of gill, mantle and hepatopancreas of ark shell sampled 0, 3, 6, 12, 24, 48 and 96 hours after the transferring. The results showed that with the rising of the temperature and the increase in treatment time, activities of SOD, CAT and GSH-PX in all the organs were increased at first and decreased afterwards. The maximal activities of of SOD, CAT and GSH-PX were found to be signifantly higher in mantle and hepatopancreas in 14 ℃ group and 20 ℃ group after 6 hours of treatment than that in the control(P<0.05). For the rest temperature groups the maximal activities of the three antioxidant enzymes were observed after 3 hours, significantly higher than that in the control(P<0.05). After 24 hours, activities of antioxidant enzyme of all groups recovered to the level in the control. In all temperature groups, the highest activity level of SOD, CAT and GSH-PX appeared in hepatopancreas, followed by the mantle and the minimal values in gill.
引文
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[7] 郭勤单,王有基,吕为群,等.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J].水生生物学报,2014,38(1):58-67.
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[12] 刘峰,刘阳阳,楼宝,等.温度对小黄鱼体内抗氧化酶及消化酶活性的影响[J].海洋学报,2016,38(12):76-85.
[13] 钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究[J].南方水产科学,2015,11(6):49-57.
[14] 胡静,吴开畅,叶乐,等.急性盐度胁迫对克氏双锯鱼幼鱼过氧化氢酶的影响[J].南方水产科学,2015,11(6):73-78.
[15] 李晓英,沈睿杰,董志国,等.温度骤升和窒息胁迫对青蛤抗氧化酶活性的影响[J].中国饲料,2009(1):39-42.
[16] 李晓英,董志国,阎斌伦,等.青蛤POD组织差异及温度骤升和窒息胁迫对青蛤POD的影响[J].海洋通报,2010,29(5):521-525.
[17] 王怡,胡婉彬,李家祥,等.急性盐度胁迫对紫石房蛤(Saxidomus purpurata)鳃组织结构及4种酶活性的影响[J].中国农业科技导报,2016,18(5):178-186.
[18] 邓平平,施永梅,汪洋,等.盐度对长江刀鲚幼鱼非特异性免疫酶和消化酶活性的影响[J].大连海洋大学学报,2016,31(5):533-537.
[19] Hu M H,Li L S,Sui Y M,et al.Effect of pH and temperature on antioxidant responses of the thick shell mussel Mytilus coruscus[J].Fish and Shellfish Immunology,2015,46(2):573-583.
[20] 曹善茂,王潇,刘钢,等.温度胁迫对岩扇贝幼贝抗氧化酶活力的影响[J].大连海洋大学学报,2018,33(2):223-227.
[21] 傅郁.海湾扇贝外套膜组织学组织化学研究[D].青岛:中国海洋大学,2007.
[22] 吴静.温度和盐度对华贵栉孔扇贝(Mimachlamys nobilis Reeve)存活、免疫指标及生理指标的影响[D].福州:福建师范大学,2016.
[23] 李文龙,梁兴明,梁萌青,等.温度对大菱鲆幼鱼生长及免疫相关酶活性的影响[J].水产科学,2017,36(3):311-316.
[2] 沈源远,顾亚卿,苗家禄.1979年渤海湾北部海区毛蚶资源调查报告[J].天津水产,1980(7):1-5.
[3] 杨玉香,余晓亭,郑国富,等.毛蚶幼贝生活习性研究[J].水产科学,2004,23(12):18-20.
[4] 杨凤,刘丹,于倩,等.环境因子对毛蚶排遗和泥沙净化的影响[J].大连海洋大学学报,2012,27(6):523-527.
[5] 姜祖辉,王俊.温度和规格对毛蚶耗氧率和排氨率的影响[J].青岛大学学报,1999,12(1):75-79.
[6] 亢玉静.温度和盐度对西藏拟溞抗氧化酶及热休克蛋白HSP70基因表达的影响[D].大连:大连海洋大学,2013.
[7] 郭勤单,王有基,吕为群,等.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J].水生生物学报,2014,38(1):58-67.
[8] Pipe R K,Porter C,Livingstone D R.Antioxidant enzymes associated with the blood cells and hemolymph of the mussel Mytilus edulis[J].Fish and Shellfish Immunology,1993,3(3):221-233.
[9] 陈雷,张嵩,郭良勇,等.温度对毛蚶、文蛤和青蛤潜沙能力的影响[J].水产学杂志,2016,29(3):35-38.
[10] Chatterjee N,Pal A K,Manush S M,et al.Thermal tolerance and oxygen consumption of Labeo rohita and Cyprinus carpio early fingerlings acclimated to three different temperatures [J].Journal of Thermal Biology,2004,29(6):265-270.
[11] Martinez-A′lvarez R M,Morales A E,Sanz A.Antioxidant defenses in fish:biotic and abiotic factors [J].Reviews in Fish Biology and Fisheries,2005,15(1/2):75-88.
[12] 刘峰,刘阳阳,楼宝,等.温度对小黄鱼体内抗氧化酶及消化酶活性的影响[J].海洋学报,2016,38(12):76-85.
[13] 钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究[J].南方水产科学,2015,11(6):49-57.
[14] 胡静,吴开畅,叶乐,等.急性盐度胁迫对克氏双锯鱼幼鱼过氧化氢酶的影响[J].南方水产科学,2015,11(6):73-78.
[15] 李晓英,沈睿杰,董志国,等.温度骤升和窒息胁迫对青蛤抗氧化酶活性的影响[J].中国饲料,2009(1):39-42.
[16] 李晓英,董志国,阎斌伦,等.青蛤POD组织差异及温度骤升和窒息胁迫对青蛤POD的影响[J].海洋通报,2010,29(5):521-525.
[17] 王怡,胡婉彬,李家祥,等.急性盐度胁迫对紫石房蛤(Saxidomus purpurata)鳃组织结构及4种酶活性的影响[J].中国农业科技导报,2016,18(5):178-186.
[18] 邓平平,施永梅,汪洋,等.盐度对长江刀鲚幼鱼非特异性免疫酶和消化酶活性的影响[J].大连海洋大学学报,2016,31(5):533-537.
[19] Hu M H,Li L S,Sui Y M,et al.Effect of pH and temperature on antioxidant responses of the thick shell mussel Mytilus coruscus[J].Fish and Shellfish Immunology,2015,46(2):573-583.
[20] 曹善茂,王潇,刘钢,等.温度胁迫对岩扇贝幼贝抗氧化酶活力的影响[J].大连海洋大学学报,2018,33(2):223-227.
[21] 傅郁.海湾扇贝外套膜组织学组织化学研究[D].青岛:中国海洋大学,2007.
[22] 吴静.温度和盐度对华贵栉孔扇贝(Mimachlamys nobilis Reeve)存活、免疫指标及生理指标的影响[D].福州:福建师范大学,2016.
[23] 李文龙,梁兴明,梁萌青,等.温度对大菱鲆幼鱼生长及免疫相关酶活性的影响[J].水产科学,2017,36(3):311-316.