HPLC法测定补骨脂-青龙衣酊中补骨脂素的含量
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摘要
目的:建立测定补骨脂-青龙衣酊中补骨脂素含量的方法。方法:采用高效液相色谱法。色谱柱为Shim-pack VP-ODS C18,流动相为甲醇-水(60∶40,V/V),流速为0.8 ml/min,检测波长为246 nm,柱温为25℃,进样量为20μl。结果:补骨脂素的检测质量浓度线性范围为2.49~39.90μg/ml;精密度、稳定性、重复性试验的RSD<2.00%;加样回收率范围为97.23%~100.58%,RSD=1.17%(n=6)。结论:该方法快速简便、准确可靠,可用于补骨脂-青龙衣酊中补骨脂素的含量测定。
OBJECTIVE:To establish a method for the content determination of psoralen in Psoralea-pericarpium juglates liniment. METHODS:HPLC was performed on the column of Shim-pack VP-ODS C18 with mobile phase of methanol-water(60∶40,V/V)at a flow rate of 0.8 ml/min,detection wavelength was 246 nm,column temperature was 25 ℃,and injection volume was 20μl. RESULTS:The linear range of psoralen was 2.49-39.90 μg/ml;RSDs of precision,stability and reproducibility tests were lower than 2.00%;recovery was 97.23%-100.58%(RSD=1.17%,n=6). CONCLUSIONS:The method is fast,simple,accurate and reliable,and can be used for the content determination of psoralen in Psoralea-pericarpium juglates liniment.
OBJECTIVE:To establish a method for the content determination of psoralen in Psoralea-pericarpium juglates liniment. METHODS:HPLC was performed on the column of Shim-pack VP-ODS C18 with mobile phase of methanol-water(60∶40,V/V)at a flow rate of 0.8 ml/min,detection wavelength was 246 nm,column temperature was 25 ℃,and injection volume was 20μl. RESULTS:The linear range of psoralen was 2.49-39.90 μg/ml;RSDs of precision,stability and reproducibility tests were lower than 2.00%;recovery was 97.23%-100.58%(RSD=1.17%,n=6). CONCLUSIONS:The method is fast,simple,accurate and reliable,and can be used for the content determination of psoralen in Psoralea-pericarpium juglates liniment.
引文
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[16]周岚,乙引,伍庆,等.HPLC同时测定仙灵骨葆胶囊中朝藿定C、淫羊藿苷、补骨脂素和异补骨脂素[J].中草药,2011,42(10):1 998.
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[19]唐丽琴,张善堂,陈象青,等.HPLC法测定不同来源补骨脂中补骨脂素和异补骨脂素的含量[J].中国药师,2011,14(3):377.
[2]周峻伟,姚卫海,郭玉红,等.从肝论治白癜风病因病机浅析[J].实用中医内科杂志,2009,23(10):6.
[3]于晓梅.白癜风从肝肾论治90例临床观察[J].北京中医杂志,2002,21(6):345.
[4]Krüger C,Schallreuter KU.A review of the worldwide prevalence of vitiligo in children/adolescents and adults[J].Int J Dermatol.2012,51(10):1 206.
[5]赵瑞亭,陈晓明,侯晓军.补骨脂-青龙衣酊的制备工艺研究[J].中国药房,2008,19(30):2 349.
[6]熊鸿燕.补骨脂素的光敏反应[J].中国消毒学杂志,2002,19(4):231.
[7]吴疆,魏巍,袁永兵.补骨脂的化学成分和药理作用研究进展[J].药物评价研究,2011,34(3):217.
[8]李洪武,朱文元.治疗白癜风复方中药体外对蘑菇酪氨酸酶活性的作用[J].临床皮肤科杂志,2000,29(3):133.
[9]黄玥,胡卫平,赵东保,等.核桃青果皮色素成分分析及稳定性研究[J].中国农学通报,2012,28(30):272.
[10]李淑芳,孙光春,鲍淑娟,等.核桃皮酊剂毒性试验研究[J].贵阳医学院学报,1997,22(4):356.
[11]余平,刘长姣,乔春峰,等.利用超高压技术提取补骨脂中补骨脂素和异补骨脂素[J].食品科学,2011,32(20):39
[12]卢彦芳,安静,蒋晔.微波萃取快速分析补骨脂中补骨脂素和异补骨脂素[J].中国中药杂志,2011,36(9):1 158.
[13]刘素玲,蔡益锐.HPLC法测定归芍通络合剂中补骨脂素、异补骨脂素的含量[J].中国药师,2012,15(5):668.
[14]覃建明,莫文电,谭忠谋.RP-HPLC法测定老年咳喘片中补骨脂素和异补骨脂素的含量[J].世界中医药,2010,5(3):221.
[15]任青花,赵剑锋,赵承孝.白癜风浓缩丸质量标准的研究[J].山西医科大学学报,2012,43(4):268.
[16]周岚,乙引,伍庆,等.HPLC同时测定仙灵骨葆胶囊中朝藿定C、淫羊藿苷、补骨脂素和异补骨脂素[J].中草药,2011,42(10):1 998.
[17]宣铁锋.高效液相色谱法测定生发酊中补骨脂素和异补骨脂素含量[J].中国药业,2012,21(18):37.
[18]张婷,龚志成,唐芬芬.补骨脂微乳液中补骨脂素与异补骨脂素的含量测定[J].医药导报,2012,31(12):1 619.
[19]唐丽琴,张善堂,陈象青,等.HPLC法测定不同来源补骨脂中补骨脂素和异补骨脂素的含量[J].中国药师,2011,14(3):377.