超高效液相色谱-质谱法测定药包材中的双酚A
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摘要
目的建立超高效液相色谱-串联三重四级杆质谱联用测定药品包装材料中双酚A的检测方法。方法采用BEH C_(18)液相柱分离,流动相为乙腈和水,采用梯度洗脱方式,电喷雾离子源,负离子扫描,多反应监测模式进行质谱分析。结果 BPA在0.5～106.3 ng·mL~(-1)范围内,线性关系良好(r=0.999 9)。结论该方法操作简便,灵敏度高,能满足药品包装材料中双酚A含量的测定。
Objective To establish a method for the determination of bisphenol A in pharmaceutical packaging materials by ultrahigh performance liquid chromatography-tandem triple quadruple mass spectrometry.Methods BEH C_(18) column was used to separate acetonitrile and water as mobile phase.Gradient elution method,electrospray ion source,negative ion scanning and multi-reaction monitoring mode were used for mass spectrometry analysis.Results The linear calibration curve of bisphenol A was obtained in the concentration range of 0.5～106.3 ng·mL~(-1)(r=0.999 9).Conclusion The method was simple,sensitive and can be used to determine the content of bisphenol A in pharmaceutical packaging materials.
Objective To establish a method for the determination of bisphenol A in pharmaceutical packaging materials by ultrahigh performance liquid chromatography-tandem triple quadruple mass spectrometry.Methods BEH C_(18) column was used to separate acetonitrile and water as mobile phase.Gradient elution method,electrospray ion source,negative ion scanning and multi-reaction monitoring mode were used for mass spectrometry analysis.Results The linear calibration curve of bisphenol A was obtained in the concentration range of 0.5～106.3 ng·mL~(-1)(r=0.999 9).Conclusion The method was simple,sensitive and can be used to determine the content of bisphenol A in pharmaceutical packaging materials.
引文
[1] 胡珊珊,申秀英,许晓路,等.双酚A的毒理学效应及其作用机制的研究进展[J].江西科学,2006,24(5):383-386.
[2] 王佳,詹平.双酚A对机体影响及其机制的研究进展[J].预防医学情报杂志,2005,21(5):541-544.
[3] 郑力行,石峻岭,周志俊.高效液相色谱法测定血清中的双酚A[J].中华预防医学杂志,2003,37(6):450-451.
[4] 陈志锋,刘晓华,孙利.高效液相色谱法测定食品中的双酚类物质[J].检验检疫学刊,2009,19(6):9-12.
[5] 杨红梅,王浩,刘艳琴,等.液相色谱-串联三重四极杆质谱测定饮料中双酚A残留[J].食品研究与开发,2009,30(12):137-139.
[6] 唐熙,陈高群,梁鸣,等.气相色谱法测定塑料奶瓶中迁移出的双酚A[J].福建分析测试,2010,19(4):13-17.
[7] 卫碧文,缪俊文,于文佳.气相色谱-质谱法分析食品包装材料中双酚A[J].分析试验室,2009,28(1):107-109.
[8] 蒋小良,曾铭,郝雨,等.超高效液相色谱-质谱法快速测定塑料包装饮料中双酚A[J].分析测试技术与仪器,2013,19(2):77-82.
[2] 王佳,詹平.双酚A对机体影响及其机制的研究进展[J].预防医学情报杂志,2005,21(5):541-544.
[3] 郑力行,石峻岭,周志俊.高效液相色谱法测定血清中的双酚A[J].中华预防医学杂志,2003,37(6):450-451.
[4] 陈志锋,刘晓华,孙利.高效液相色谱法测定食品中的双酚类物质[J].检验检疫学刊,2009,19(6):9-12.
[5] 杨红梅,王浩,刘艳琴,等.液相色谱-串联三重四极杆质谱测定饮料中双酚A残留[J].食品研究与开发,2009,30(12):137-139.
[6] 唐熙,陈高群,梁鸣,等.气相色谱法测定塑料奶瓶中迁移出的双酚A[J].福建分析测试,2010,19(4):13-17.
[7] 卫碧文,缪俊文,于文佳.气相色谱-质谱法分析食品包装材料中双酚A[J].分析试验室,2009,28(1):107-109.
[8] 蒋小良,曾铭,郝雨,等.超高效液相色谱-质谱法快速测定塑料包装饮料中双酚A[J].分析测试技术与仪器,2013,19(2):77-82.