分离自土壤Paraboeremia属真菌一新记录种
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摘要
采集贵州贵阳花溪湿地公园山林土壤,分离得到Paraboeremia属真菌一新记录种,对该种结合形态特征及分子系统学分析基于ITS,LSU,rpb2和tub四个基因片段,在系统进化树上,该标本HGUP1801与采自荷兰卷柏叶片上的Paraboeremia selaginellae聚集在一起,并且形成很高的支持率,将该种定名为Paraboeremia selaginellae。研究标本保存在贵州大学植物病理学实验室(HGUP)。
A newly recorded strain of the genus Paraboeremia was isolated from the soil of the woodland of Huaxi Wetland Park in Guiyang, Guizhou.This species was identified by combining morphological characters and phylogenetic analysis based on sequences of Internal Transcribed Spacer(ITS),Ribosomal Large Subunit(LSU),RNA polymeraseⅡ largest subunit 2(rpb2)andβ-tubulin(tub).The phylogenetic analysis revealed that the strain HGUP1801 was clustered with Paraboeremia selaginellae isolated from the leaves of Selaginellaat Netherland. The strain was strongly supported to be identified as Paraboeremia selaginellae. The holotype and ex-type cultures are preserved in the Plant Pathology Laboratory of Guizhou University.(HGUP).
A newly recorded strain of the genus Paraboeremia was isolated from the soil of the woodland of Huaxi Wetland Park in Guiyang, Guizhou.This species was identified by combining morphological characters and phylogenetic analysis based on sequences of Internal Transcribed Spacer(ITS),Ribosomal Large Subunit(LSU),RNA polymeraseⅡ largest subunit 2(rpb2)andβ-tubulin(tub).The phylogenetic analysis revealed that the strain HGUP1801 was clustered with Paraboeremia selaginellae isolated from the leaves of Selaginellaat Netherland. The strain was strongly supported to be identified as Paraboeremia selaginellae. The holotype and ex-type cultures are preserved in the Plant Pathology Laboratory of Guizhou University.(HGUP).
引文
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[2] Jiang JR,Chen Q,Cai L.Polyphasic characterisation of three novel species of Paraboeremia[J].Mycological Progress,2016,16(4):285-295.
[3] Aveslcamp MM,de Gruyter J,Woudenberg JHC,et al.Highlights of the Didymellaceae:A polyphasic approach to characterise Phoma and related pleosporalean genera[J].Studies in Mycology,2010,65(1):1-60.
[4] Aveskamp MM,deGruyter J,Crous PW,Biology and recent developments in the systematics of Phoma.a complex genus of major quarantine significance[J].Fungal Diversity,2008(31):1-18.
[5] de Gruyter J,Woudenberg JHC,Aveslcamp MM,et al.Molecular phylogeny of Phoma and allied anamorph genera:Towards a reclassification of the Phoma complex[J].Mycological Research,2009,113(4):508-519.
[6] Aveskamp MM,Johannes DG,Monica AM,et al.DNA phylogeny reveals polyphyly of Phoma section Peyronellaea and multiple taxonomic novelties[J].Mycologia,2009,101(3):363-382.
[7] De Gruyter J,Woudenberg JHC,Aveskamp MM,et al.Systematic reappraisal of species in Phoma section Paraphoma,Pyrenochaeta and Pleurophoma[J].Mycologia,2010,102(5):1066-1081.
[8] De Gruyter J,Woudenberg JHC,Aveskamp MM,et al.Redisposition of phoma-like anamorphs in Pleosporales[J].Studies in Mycology,2012(75):1-36.
[9] Chen Q,Hou LW,Duan WJ,et al.Didymellaceae revisited[J].Studies in Mycology,2017(87):105-159.
[10] Valenzuela-Lopez N,Cano-Lira JF,Guarro J, et al. Coelomycetous Dothideomycetes with emphasis on the families Cucurbitariaceae and Didymellaceae[J]. Studies in Mycology,2018(90):1-69.
[11] White TJ,Bruns T,Lee S.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.In:PCR Protocols:a guide to methods and applications[M].California:Academic Press,1990:315-322.
[12] Vilgalys R,Hester M,Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species[J].Journal of Bacteriology,1990,172(8):4238-4246.
[13] Liu YJ,Whelen S,Hall BD,Phylogenetic relationships among ascomycetes evidence from an RNA polymerase II subunit[J].Molecular Biology and Evolution, 1999,16(12):1799-1808.
[14] Boerema GH,de Gruyter J,Noordeloos ME,New names in Phoma[J].Persoonia,1995,16(1):131-131.
[15] 蔡琼,丁贵杰,森林土壤生态环境质量评价指标研究概述[J].山地农业生物学报,2006,25(3):256-261.