刚度对肿瘤细胞生物学行为影响的研究进展
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摘要
刚度在医学领域用来描述生物组织力学特性,不同组织器官的刚度差别很大,而相同组织器官的刚度在一定范围内波动。正常组织与其相应部位肿瘤组织的刚度截然不同,肿瘤细胞的刚度较相应的正常组织要小。刚度对肿瘤细胞增殖、迁移、分化等生物学行为存在重要影响,甚至可以影响肿瘤细胞对放射治疗及化学治疗的反应。从刚度这一生物力学特性研究角度出发,可以丰富人们对肿瘤形成机制的认识,为临床有效防治肿瘤提供新方向。
Stiffness is used to describe the mechanical properties of biological tissues in medicine. The stiffness of different tissues and organs is different,and of the same tissues and organs fluctuates in a certain range. Moreover,the stiffness of normal tissue is quite different from that of its corresponding tumor tissue that tumor cells' stiffness is lower. Stiffness has important effects on biological behavior such as proliferation,migration and differentiation of tumor cells,and even affects the response of tumor cells to radiotherapy and chemotherapy. The angle of biomechanical characteristics of stiffness,can enrich the understanding of the mechanism of tumor formation and provide a new direction for effective prevention and treatment of tumors.
Stiffness is used to describe the mechanical properties of biological tissues in medicine. The stiffness of different tissues and organs is different,and of the same tissues and organs fluctuates in a certain range. Moreover,the stiffness of normal tissue is quite different from that of its corresponding tumor tissue that tumor cells' stiffness is lower. Stiffness has important effects on biological behavior such as proliferation,migration and differentiation of tumor cells,and even affects the response of tumor cells to radiotherapy and chemotherapy. The angle of biomechanical characteristics of stiffness,can enrich the understanding of the mechanism of tumor formation and provide a new direction for effective prevention and treatment of tumors.
引文
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[2]Engler AJ,Griffin MA,Sen S,et al.Myotubes differentiate optimally on substrates with tissue-like stiffness:Pathological implications for soft or stiff microenvironments[J].J Cell Biol,2004,166(6):877-887.
[3]Yeung T,Georges PC,Flanagan LA,et al.Effects of substrate stiffness on cell morphology,cytoskeletal structure,and adhesion[J].Cell Motil Cytoskeleton,2005,60(1):24-34.
[4]Bade ND,Kamien RD,Assoian RK,et al.Curvature and Rho activation differentially control the alignment of cells and stress fibers[J].Sci Adv,2017,3(9):e1700150.
[5]Leventa I,Georgesa PC,Janmey PA,et al.Soft biological materials and their impact on cell function[J].Soft Matter,2007,3:299-306.
[6]Ji T,Zhao Y,Ding Y,et al.Using functional nanomaterials to target and regulate the tumor microenvironment:Diagnostic and therapeutic applications[J].Adv Mater,2013,25(26):3508-3525.
[7]Shieh AC.Biomechanical forces shape the tumor microenvironment[J].Ann Biomed Eng,2011,39(5):1379-1389.
[8]Wang M,Chai N,Sha B,et al.The effect of substrate stiffness on cancer cell volume homeostasis[J].J Cell Physiol,2017,233(2):1414-1423.
[9]Butcher DT,Alliston T,Weaver VM.A tense situation:Forcing tumour progression[J].Nat Rev Cancer,2009,9(2):108-122.
[10]Levental KR,Yu H,Kass L,et al.Matrix crosslinking forces tumor progression by enhancing integrin signaling[J].Cell,2009,139(5):891-906.
[11]Stanton MM,Rankenberg JM,Park BW,et al.Cell behavior on surface modified polydimethylsiloxane(PDMS)[J].Macromol Biosci,2014,14(7):953-964.
[12]Zhou B,Gao X,Wang C,et al.Functionalized PDMS with versatile and scalable surface roughness gradients for cell culture[J].ACS Appl Mater Interfaces,2015,7(31):17181-17187.
[13]Wang T,Lai JH,Han LH,et al.Chondrogenic differentiation of adipose-derived stromal cells in combinatorial hydrogels containing cartilage matrix proteins with decoupled mechanical stiffness[J].Tissue Eng Part A,2014,20(15/16):2131-2139.
[14]Lange JR,Fabry B.Cell and tissue mechanics in cell migration[J].Exp Cell Res,2013,319(16):2418-2423.
[15]Suresh S.Biomechanics and biophysics of cancer cells[J].Acta Biomater,2007,3(4):413-438.
[16]Omidvar R,Tafazzoli-Shadpour M,Shokrgozar MA,et al.Atomic force microscope-based single cell force spectroscopy of breast cancer cell lines:An approach for evaluating cellular invasion[J].J Biomech,2014,47(13):3373-3379.
[17]Ramos JR,Pabijan J,Garcia R,et al.The softening of human bladder cancer cells happens at an early stage of the malignancy process[J].Beilstein J Nanotechnol,2014,5:447-457.
[18]Hayashi K,Iwata M.Stiffness of cancer cells measured with an AFM indentation method[J].J Mech Behav Biomed Mater,2015,49:105-111.
[19]Zhao X,Zhong Y,Ye T,et al.Discrimination between cervical cancer cells and normal cervical cells based on longitudinal elasticity using atomic force microscopy[J].Nanoscale Res Lett,2015,10(1):482-483.
[20]Cross SE,Jin YS,Rao J,et al.Nanomechanical analysis of cells from cancer patients[J].Nat Nanotechnol,2007,2(12):780-783.
[21]Bastatas L,Martinez-Marin D,Matthews J,et al.AFM nanomechanics and calcium dynamics of prostate cancer cells with distinct metastatic potential[J].Biochim Biophys Acta,2012,1820(7):1111-1120.
[22]Lo CM,Wang HB,Dembo M,et al.Cell movement is guided by the rigidity of the substrate[J].Biophys J,2000,79(1):144-152.
[23]Ladoux B,Mège RM.Mechanobiology of collective cell behaviours[J].Nat Rev Mol Cell Biol,2017,18(12):743-757.
[24]Feng J,Tang Y,Xu Y,et al.Substrate stiffness influences the outcome of antitumor drug screening in vitro[J].Clin Hemorheol Microcirc,2013,55(1):121-131.
[25]Liu C,Li X,Hua W,et al.Porous matrix stiffness modulates response to targeted therapy in breast carcinoma[J].Small,2016,12(34):4675-4681.
[26]Schrader J,Gordon-Walker TT,Aucott RL,et al.Matrix stiffness modulates proliferation,chemotherapeutic response,and dormancy in hepatocellular carcinoma cells[J].Hepatology,2011,53(4):1192-1205.
[27]Leight JL,Wozniak MA,Chen S,et al.Matrix rigidity regulates a switch between TGF-beta1-induced apoptosis and epithelia-mesenchymal transition[J].Mol Biol Cell,2012,23(5):781-791.
[28]Tzima E,Irani-Tehrani M,Kiosses WB,et al.A mechanosensory complex that mediates the endothelial cell response to fluid shear stress[J].Nature,2005,437(7057):426-431.
[29]Lin HH,Lin HK,Lin IH,et al.Mechanical phenotype of cancer cells:Cell softening and loss of stiffness sensing[J].Oncotarget,2015,6(25):20946-20958.
[30]Kass L,Erler JT,Dembo M,et al.Mammary epithelial cell:Influence of extracellular matrix composition and organization during development and tumorigenesis[J].Int J Biochem Cell Biol,2007,39(11):1987-1994.
[31]Provenzano PP,Inman DR,Eliceiri KW,et al.Collagen density promotes mammary tumor initiation and progression[J].BMC Med,2008,6:11.
[32]Wenstrup RJ,Florer JB,Brunskill EW,et al.Type V collagen controls the initiation of collagen fibril assembly[J].J Biol Chem,2004,279(51):53331-53337.
[33]Sharma S,Santiskulvong C,Bentolila LA,et al.Correlative nanomechanical profiling with super-resolution F-actin imaging reveals novel insights into mechanisms of cisplatin resistance in ovarian cancer cells[J].Nanomedicine,2012,8(5):757-766.
[34]Hui L,Zhang J,Ding X,et al.Matrix stiffness regulates the proliferation,stemness and chemoresistance of laryngeal squamous cancer cells[J].Int J Oncol,2017,50(4):1439-1447.