玛咖结合运动疗法对糖尿病大鼠肾线粒体呼吸链酶活性及肾功能的影响
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摘要
目的观察玛咖结合运动疗法对糖尿病大鼠肾线粒体呼吸酶活性及肾功能的影响。方法将40只糖尿病模型大鼠随机分为模型对照组、玛咖组、运动组和玛咖+运动组。玛咖组和玛咖+运动组每日灌胃玛咖粉剂(5 g/kg),运动组和玛咖+运动组进行跑台训练。6周后各组大鼠进行力竭运动(35 m/min),随后即刻麻醉提取肾线粒体,测定线粒体呼吸链酶RCCI、RCCII、RCCIII、RCCIV活性及血尿素氮(blood urea nitrogen,BUN)、血清肌酐(serum creatinine,SCr)、血糖(blood glucose)含量。结果与对照组比较,玛咖组大鼠肾线粒体呼吸链酶RCCII,运动组RCCI、RCCII,玛咖+运动组RCCI、RCCII、RCCIV活性显著升高;与玛咖组比较,玛咖+运动组RCCI、RCCII活性显著升高;与运动组比较,玛咖+运动组RCCII活性显著升高。与对照组比较,玛咖组、玛咖+运动组大鼠血尿素氮含量,运动组、玛咖+运动组血清肌酐、血糖水平降低,差异有显著性(P<0. 05或P<0. 01);与玛咖组比较,玛咖+运动组血清肌酐、血糖水平降低,差异有显著性(P<0. 05);与运动组比较,玛咖+运动组血尿素氮、血糖水平降低,差异有显著性(P<0. 05)。结论玛咖结合运动疗法可以提高老龄糖尿病大鼠肾线粒呼吸功能,提高机体氧化磷酸化水平,改善肾机能。
Objective To observe the effect of Maca combined exercise therapy on mitochondrial respiratory enzyme activity and renal function in diabetic rats. Methods Forty diabetic rats were randomly divided into model control group,Maca group,training group and Maca + training group. The training and Maca + training groups underwent treadmill exercise training every day( 5 g/kg). After 6 weeks,rats in each group were subjected to exhaustive exercise( 35 m/min)followed by immediate extraction of renal mitochondria under anesthesia. Activity of the mitochondrial respiratory chain enzymes RCCI,RCCII,RCCIII and RCCIV,blood urea nitrogen( BUN),serum creatinine( SCr),and blood glucose contents were measured. Results Compared with the control group,the Maca group RCCII,training group RCCI and RCCII,and Maca + training group RCCI,RCCII and RCCIV activities were significantly increased. Compared with the Maca group,RCCI and RCCII activities in the Maca + training group were significantly increased. Compared with the training group,the RCCII activity in the Maca + training group was significantly increased. Compared with the control group,the content of blood urea nitrogen in the Maca and Maca + training groups,and the levels of serum creatinine and blood glucose in the training group and Maca + training group were significantly decreased( P < 0. 05 or P < 0. 01).Compared with the Maca group,the levels of serum creatinine and blood glucose were significantly decreased in the Maca +training group( P < 0. 05). Compared with the training group,the levels of blood urea nitrogen and blood glucose in the Maca + training group were significantly decreased( P < 0. 05). Conclusions Maca combined with exercise therapy improved the respiratory function,increased the level of oxidative phosphorylation,and improved kidney function in aged diabetic rats.
Objective To observe the effect of Maca combined exercise therapy on mitochondrial respiratory enzyme activity and renal function in diabetic rats. Methods Forty diabetic rats were randomly divided into model control group,Maca group,training group and Maca + training group. The training and Maca + training groups underwent treadmill exercise training every day( 5 g/kg). After 6 weeks,rats in each group were subjected to exhaustive exercise( 35 m/min)followed by immediate extraction of renal mitochondria under anesthesia. Activity of the mitochondrial respiratory chain enzymes RCCI,RCCII,RCCIII and RCCIV,blood urea nitrogen( BUN),serum creatinine( SCr),and blood glucose contents were measured. Results Compared with the control group,the Maca group RCCII,training group RCCI and RCCII,and Maca + training group RCCI,RCCII and RCCIV activities were significantly increased. Compared with the Maca group,RCCI and RCCII activities in the Maca + training group were significantly increased. Compared with the training group,the RCCII activity in the Maca + training group was significantly increased. Compared with the control group,the content of blood urea nitrogen in the Maca and Maca + training groups,and the levels of serum creatinine and blood glucose in the training group and Maca + training group were significantly decreased( P < 0. 05 or P < 0. 01).Compared with the Maca group,the levels of serum creatinine and blood glucose were significantly decreased in the Maca +training group( P < 0. 05). Compared with the training group,the levels of blood urea nitrogen and blood glucose in the Maca + training group were significantly decreased( P < 0. 05). Conclusions Maca combined with exercise therapy improved the respiratory function,increased the level of oxidative phosphorylation,and improved kidney function in aged diabetic rats.
引文
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[28]查圣华.玛咖活性成分及抗疲劳功能研究[D].中国科学院大学,2016:15-36.
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[2]黄敏,赵玲玲.呼吸链酶复合体Ⅰ研究进展[J].广东医学,2014,32(5):662-664.
[3]楚璐雅.玛咖对人体抗氧化能力、免疫功能及血脂代谢的影响[D].北京体育大学,2016:8-30.
[4]柳琴,程贝.西藏玛咖对小鼠抗疲劳作用的初步研究[J].中国药师,2015,18(3):511-513.
[5]彭丽娜,贾鹏.玛咖对力竭运动后老年大鼠肾脏线粒体呼吸功能及抗衰老能力的影响[J].中国组织工程研究,2016,20(18):2711-2716.
[6]彭丽娜,张冰.玛咖对老年大鼠脑组织线粒体呼吸链酶活性及抗衰老能力的影响[J].中国老年学杂志,2018,38(1):50-52.
[7]彭丽娜,彭丽妍.玛咖结合运动对阿毒素诱导心肌炎大鼠心肌线粒体呼吸链酶活性及抗氧化损伤能力的影响[J].临床心血管病杂志,2016,32(8):853-856.
[8]邵俊伟,蔡逊.高脂饮食联合链脲佐菌素建立2型糖尿病大鼠模型的研究进展[J].中国实验动物学报,2014,22(4):90-93.
[9]朱超,朱莹莹.Ⅱ型糖尿病动物模型的构建[J].中国实验动物学报,2013,21(2):84-87.
[10]田野,高铁群.大鼠运动性疲劳模型的建立[J].北京体育大学学报,1995,18(4):49-53.
[11]高玉秋,李彬,王彦武,等.玛咖片缓解体力疲劳功能的实验研究[J].中国食物与营养,2014,20(8):76-77.
[12] Zwicker K,Dikalov S,Matuschka S,et al. Oxygen radical generation and enzymatic properties of mitochondria in hypoxia/reoxygenation[J]. Arzneimittel-Forschung,1998,48(6):629-636.
[13] Vyatkina G,Bhatia V,Gerstner A,et al. Impaired mitochondrial respiratory chain and bioenergetics during chagasic cardiomyopathy development[J]. Biochim Biophys Acta,2004,1689(2):162-173.
[14]习雪峰.运动和EGCG对Ⅱ型糖尿病大鼠海马线粒体功能的改善作用及机制研究[D].苏州大学,2014:20-80.
[15]陈德明,牛衍龙.糖尿病运动治疗方案中运动项目选择的研究[J].哈尔滨体育学院学报,2014,32(2):87-96.
[16]刘银银.有氧运动对老龄大鼠心肌线粒体能量代谢及自由基代谢的影响[D].西北师范大学,2014:21-26.
[17]朱洪竹,肖国强,朱梅菊,等.有氧运动对2型糖尿病大鼠肾脏VEGF-A及VEGFR2蛋白表达的影响[J].上海体育学院学报,2015,39(6):61-67,83.
[18]林子桐,张超,沈雪梅.糖尿病肾病发病机制研究进展[J].中国药理学与毒理学杂志,2014,28(5):765-771.
[19]刘芳,杨华,周文江,等.诱发性2型糖尿病小鼠模型与自发性db/db小鼠特性的比较[J].中国实验动物学报,2014,22(6):54-59.
[20]陈木,唐赵平.短期有氧运动对糖尿病大鼠肾功能的影响[J].上饶师范学院学报,2014,34(6):111-115.
[21]李一梅,刘宽芝.氧化应激、线粒体功能障碍与2型糖尿病[J].临床荟萃,2014,29(2):213-215.
[22]汤春雪.天然玛咖制剂对运动员身体机能状态影响的功效研究[D].北京体育大学,2016:20-30.
[23]许敏,徐丽,宋辉,等.玛咖的研究进展[J].食品安全质量检测学报,2015,6(7):2775-2781.
[24]何黛,侯改霞.有氧运动对糖尿病肾病大鼠肾功能影响研究[J].北京体育大学学报,2009,32(12):66-68.
[25]姜勤佳,陈雅,袁春华.不同强度有氧运动对糖尿病肾病疾病大鼠肾功能影响研究[J].山西体育科技,2012,32(3):15-18.
[26]朱竟赫,赵金明,秦文艳,等.高脂血症实验动物模型研究概述[J].实验动物科学,2012,29(2):48-52.
[27]谈文博.适度运动对增龄大鼠骨骼肌线粒体能量代谢及自由基代谢的影响[D].西北师范大学,2014:15-20.
[28]查圣华.玛咖活性成分及抗疲劳功能研究[D].中国科学院大学,2016:15-36.
[29]罗曦娟,王正珍,朱玲,等.有氧和抗阻运动对糖尿病前期人群血糖干预效果的比较研究[J].中国运动医学杂志,2015,34(9):831-837.