生物超微弱发光在针刺研究中的应用思路
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摘要
生物超微弱发光(UPE)是广泛存在于包括人类、动物、植物等一切生物生命活动中的生理现象,它反映了生物体的能量代谢现象。从20世纪开始,UPE就已经在中医经络腧穴机制研究中有所应用,主要的发现为人体某些穴位和经络具有高发光的特性,但想通过UPE技术展现穴位及经络真实性的问题还尚未解决。现基于UPE仪器采集信号及成像系统的发展,拟从人体穴位与非穴位、针刺前后穴位发光强度及延迟效应方面入手,对人体穴位的UPE现象及实质继续进行探究。本文就其在针刺效应研究中的应用进行初步探讨,并就其在经络腧穴方面的应用进行展望。
Ultra-weak bioluminescence(UWL)is a physiological phenomenon widely existing in all the biological activities including human,animals,plants,etc.,which reflects the energy metabolism of the organism.Since the last century,ultraweak photon emission(UPE)has been applied to the study of the essence of meridians and acupoints of traditional Chinese medicine and obtained some results as the higher luminescence characteristics,but many problems remain unsolved due to the limitation of detection technology.In recent years,along with the development of bioluminescence signal acquiring system and imaging system,we are able to further explore the characteristics and biological mechanisms of UWL of acupuncture points and meridians in the human body.We proposed to study changes of ultra-weak luminous intensity of acupuncture points and meridians before and after needling stimulation,and the delayed effect of UPE phenomenon,etc.,trying to reveal their regularities and essence.In this paper,the prospect of application of UPE to acupuncture research is also discussed by combining newly acquired results of some biological substances of acupoints in experimental studies.
Ultra-weak bioluminescence(UWL)is a physiological phenomenon widely existing in all the biological activities including human,animals,plants,etc.,which reflects the energy metabolism of the organism.Since the last century,ultraweak photon emission(UPE)has been applied to the study of the essence of meridians and acupoints of traditional Chinese medicine and obtained some results as the higher luminescence characteristics,but many problems remain unsolved due to the limitation of detection technology.In recent years,along with the development of bioluminescence signal acquiring system and imaging system,we are able to further explore the characteristics and biological mechanisms of UWL of acupuncture points and meridians in the human body.We proposed to study changes of ultra-weak luminous intensity of acupuncture points and meridians before and after needling stimulation,and the delayed effect of UPE phenomenon,etc.,trying to reveal their regularities and essence.In this paper,the prospect of application of UPE to acupuncture research is also discussed by combining newly acquired results of some biological substances of acupoints in experimental studies.
引文
[1]LISTED N.Biophoton emission[J].Experientia,1988,44(7):543-600.
[2]DEVARAJ B,USA M,INABA H.Biophotons:ultraweak light emission from living systems[J].Current Opin Solid Stat Mat Sci,1997,2(2):188-193.
[3]严智强,程静.体表超微弱冷光信息规律在中医“证”,针刺得气和腧穴特性研究[J].北京中医药,1993,12(1):51-53.
[4]顾樵.生命系统的超弱光子辐射[J].科学,1989,75(1):35-40.
[5]冯前进,刘润兰.生物光子辐射中的阴阳相互作用机制[J].山西中医学院学报,2015,16(1):15-17.
[6]张晓辉,余宁梅,刘锴.基于LED的生物延迟发光检测系统[J].仪器仪表学报,2010,31(8):1878-1884.
[7]TEIJIRO I,MITSUO H,NOBUYUKI H,et al.Two-dimensional imaging of ultra-weak emission from intact soybean roots[J].Photochemist Photobiol,1989,50(3):283-286.
[8]BINKOH Y,YOSHIO G,KATSURO S,et al.Ultra-weak chemiluminescence of smokersblood[J].Arch Environmental Health Intern J,1985,40(3):148.
[9]TAKEDA M,TANNO Y,KOBAYASHI M,et al.A novel method of assessing carcinoma cell proliferation by biophoton emission[J].Cancer Lett,1998,127(1/2):155-160.
[10]MAKINO T,KATO K,LYOZUMI H,et al.Ultraweak luminescence generated by sweet potato and fusarium oxysporum interactions associated with a defense response[J].Photochemist Photobiol,1996,64(6):953-956.
[11]XING D,TAN S,TANG Y,et al.Observation of biophoton emission from plants in the process of defense response[J].Sci Bull,1999,44(23):2159-2162.
[12]GURWITSCH A.Die natur des spezifischen erregars der zellteilung[J].Arch Entw,1923,100(1/2):11-40.
[13]COLLI L,FACCHINI U.Light emission by germinating plants[J].Nuovo Cimento,1954,12(1):150-153.
[14]RUTH B,POPP F A.Experimentelle untersuchungen zur ultraschwachen photonen-emission biologischer systeme/experimental investigations on ultraweak photonemission from biological systems[J].Zeitschrift Für Naturforschung C,1976,31(11/12):741-745.
[15]RATTEMEYER M,POPP F A,NAGL W.Evidence of photon emission from DNA in living systems[J].Sci Nat,1981,68(11):572-573.
[16]王维江,韩俊英.生物超弱发光机制及其检测方法研究进展[J].广东工业大学学报,2000,17(1):49-54.
[17]POPP F A,LI K H,GU Q.Recent advances in biophoton research and its applications[M].Singapore:World Scientific,1992:46-47.
[18]强红,王香凤,朱若华,等.生物超微弱发光现象及其应用[J].化学教育,2006,27(2):6-9.
[19]郝洪升,程宝泉,张尚忠,等.原发性肝癌、肝硬化患者血清超微弱发光检测的临床研究[J].中国实用内科杂志,1996,16(7):415-415.
[20]祁李娟.血液的超微弱光子辐射和吸收光谱的研究[D].南京:南京航空航天大学,2008.
[21]SUN M,van WIJK E,KOVAL S,et al.Measuring ultraweak photon emission as a non-invasive diagnostic tool for detecting early-stage type 2diabetes:a step toward personalized medicine[J].J Photochem Photobiol B,2017,166:86-93.
[22]胡天喜.血清的超弱发光与疾病诊断[J].生物化学与生物物理进展,1988,13(1):20-24.
[23]赵占娟,王秀生,檀艳丽,等.人食管癌细胞株的超微弱发光与延迟发光[J].医学研究与教育,2009,26(2):5-7.
[24]严智强,于书庄,李君华.虚证动物模型体表冷光信息的研究[J].中医杂志,1982,26(1):72-74.
[25]张涛,杭群.针灸现代研究与临床[M].北京:中国医学出版社,1998:8.
[26]刘湘容,陈长水,余锦望,等.艾灸前后手厥阴心包经及其周边非经络组织的光传输特性探究[J].中国激光,2011,38(4):113-117.
[27]胡正发,王雄庭,张伟,等.人体小肠经及周边非经络组织的光传输特性研究[J].量子电子学报,2011,28(6):715-719.
[28]严智强,史燕清,王一中,等.人体十四正经高冷光特性的研究[J].针刺研究,1989,14(3):389-394.
[29]蒋兴慧,刘汉平,郭周义,等.经络腧穴的光学特性研究进展[J].激光生物学报,2010,19(1):127-135.
[30]严智强,舒琪,林伟力,等.子母穴冷光特性的探讨[J].山东中医药大学学报,1984,8(4):34-35.
[31]严智强,张旭良.人体体表发光的初步探讨[J].生物化学与生物物理进展,1979,6(2):50-54.
[32]杨文英,周文新,吕越,等.80例健康人体躯干部经穴超微弱发光实验研究[J].深圳中西医结合杂志,1995,1(3):1-3.
[33]杨文英,倪秀冬.原发性高血压病人经穴超微弱发光光谱观察[J].中国中医基础医学杂志,1998,4(8):49-51.
[34]严智强.体表冷光在生物医学工程学中应用的探讨[J].北京生物医学工程,1981,1(1):28-33.
[35]严智强,于书庄.循经感传与经穴发光变化之间关系的探讨[J].中医杂志,1980,30(8):55-58.
[36]池旭生,庞大本,李玉梅,等.人体体表超弱光发射的测量[J].生物物理学报,1994,10(1):165-169.
[37]PARK S H,KIM J,KOO T H.Magneto-acupuncture stimuli effects on ultraweak photon emission from hands of healthy persons[J].J Acu-Merid Stud,2009,2(1):40-48.
[38]YANG J M,CHOI C,HYUM-HEE,et al.Left-right and Yin-Yang balance of biophoton emission from hands[J].Acup Electro-therap Res,2004,29(3/4):197.
[39]韩吟华,丁光宏,沈雪勇,等.人体体表穴位点红外辐射光谱特征及其与ATP能量代谢的关系[J].生物医学工程学进展,2005,26(4):198-200.
[40]李永明.关于肥大细胞与经穴现象的“宋氏理论”及研究方向[J].中国针灸,2016,36(10):1063-1068.
[41]何伟,吴美玲,景向红,等.穴位的本态:穴位组织细胞化学的动态变化[J].中国针灸,2015,35(11):1181-1186.
[42]XU J,ZHENG S,PAN X,et al.The existence of propagated sensation along the meridian proved by neuroelectrophysiology[J].Neural Regen Res,2013,8(28):2633-2640.
[43]ZHANG W B,ZHAO Y,KJELL F,et al.Understanding propagated sensation along meridians by volume transmission in peripheral tissue[J].Chin J Integr Med,2013,19(5):330.
[44]李明伟,王频.人体经穴电学特性研究[J].中医药临床杂志,2015,27(10):1392-1394.
[45]佘延芬,齐丛会,朱江.国内外穴位电学特性研究的历史及进展评述[J].中国针灸,2010,30(12):1047-1050.
[46]STANCIULESCU R B T D.Light in shaping life[J].Homoeopathic Links,2015,28(1):64-65.
[2]DEVARAJ B,USA M,INABA H.Biophotons:ultraweak light emission from living systems[J].Current Opin Solid Stat Mat Sci,1997,2(2):188-193.
[3]严智强,程静.体表超微弱冷光信息规律在中医“证”,针刺得气和腧穴特性研究[J].北京中医药,1993,12(1):51-53.
[4]顾樵.生命系统的超弱光子辐射[J].科学,1989,75(1):35-40.
[5]冯前进,刘润兰.生物光子辐射中的阴阳相互作用机制[J].山西中医学院学报,2015,16(1):15-17.
[6]张晓辉,余宁梅,刘锴.基于LED的生物延迟发光检测系统[J].仪器仪表学报,2010,31(8):1878-1884.
[7]TEIJIRO I,MITSUO H,NOBUYUKI H,et al.Two-dimensional imaging of ultra-weak emission from intact soybean roots[J].Photochemist Photobiol,1989,50(3):283-286.
[8]BINKOH Y,YOSHIO G,KATSURO S,et al.Ultra-weak chemiluminescence of smokersblood[J].Arch Environmental Health Intern J,1985,40(3):148.
[9]TAKEDA M,TANNO Y,KOBAYASHI M,et al.A novel method of assessing carcinoma cell proliferation by biophoton emission[J].Cancer Lett,1998,127(1/2):155-160.
[10]MAKINO T,KATO K,LYOZUMI H,et al.Ultraweak luminescence generated by sweet potato and fusarium oxysporum interactions associated with a defense response[J].Photochemist Photobiol,1996,64(6):953-956.
[11]XING D,TAN S,TANG Y,et al.Observation of biophoton emission from plants in the process of defense response[J].Sci Bull,1999,44(23):2159-2162.
[12]GURWITSCH A.Die natur des spezifischen erregars der zellteilung[J].Arch Entw,1923,100(1/2):11-40.
[13]COLLI L,FACCHINI U.Light emission by germinating plants[J].Nuovo Cimento,1954,12(1):150-153.
[14]RUTH B,POPP F A.Experimentelle untersuchungen zur ultraschwachen photonen-emission biologischer systeme/experimental investigations on ultraweak photonemission from biological systems[J].Zeitschrift Für Naturforschung C,1976,31(11/12):741-745.
[15]RATTEMEYER M,POPP F A,NAGL W.Evidence of photon emission from DNA in living systems[J].Sci Nat,1981,68(11):572-573.
[16]王维江,韩俊英.生物超弱发光机制及其检测方法研究进展[J].广东工业大学学报,2000,17(1):49-54.
[17]POPP F A,LI K H,GU Q.Recent advances in biophoton research and its applications[M].Singapore:World Scientific,1992:46-47.
[18]强红,王香凤,朱若华,等.生物超微弱发光现象及其应用[J].化学教育,2006,27(2):6-9.
[19]郝洪升,程宝泉,张尚忠,等.原发性肝癌、肝硬化患者血清超微弱发光检测的临床研究[J].中国实用内科杂志,1996,16(7):415-415.
[20]祁李娟.血液的超微弱光子辐射和吸收光谱的研究[D].南京:南京航空航天大学,2008.
[21]SUN M,van WIJK E,KOVAL S,et al.Measuring ultraweak photon emission as a non-invasive diagnostic tool for detecting early-stage type 2diabetes:a step toward personalized medicine[J].J Photochem Photobiol B,2017,166:86-93.
[22]胡天喜.血清的超弱发光与疾病诊断[J].生物化学与生物物理进展,1988,13(1):20-24.
[23]赵占娟,王秀生,檀艳丽,等.人食管癌细胞株的超微弱发光与延迟发光[J].医学研究与教育,2009,26(2):5-7.
[24]严智强,于书庄,李君华.虚证动物模型体表冷光信息的研究[J].中医杂志,1982,26(1):72-74.
[25]张涛,杭群.针灸现代研究与临床[M].北京:中国医学出版社,1998:8.
[26]刘湘容,陈长水,余锦望,等.艾灸前后手厥阴心包经及其周边非经络组织的光传输特性探究[J].中国激光,2011,38(4):113-117.
[27]胡正发,王雄庭,张伟,等.人体小肠经及周边非经络组织的光传输特性研究[J].量子电子学报,2011,28(6):715-719.
[28]严智强,史燕清,王一中,等.人体十四正经高冷光特性的研究[J].针刺研究,1989,14(3):389-394.
[29]蒋兴慧,刘汉平,郭周义,等.经络腧穴的光学特性研究进展[J].激光生物学报,2010,19(1):127-135.
[30]严智强,舒琪,林伟力,等.子母穴冷光特性的探讨[J].山东中医药大学学报,1984,8(4):34-35.
[31]严智强,张旭良.人体体表发光的初步探讨[J].生物化学与生物物理进展,1979,6(2):50-54.
[32]杨文英,周文新,吕越,等.80例健康人体躯干部经穴超微弱发光实验研究[J].深圳中西医结合杂志,1995,1(3):1-3.
[33]杨文英,倪秀冬.原发性高血压病人经穴超微弱发光光谱观察[J].中国中医基础医学杂志,1998,4(8):49-51.
[34]严智强.体表冷光在生物医学工程学中应用的探讨[J].北京生物医学工程,1981,1(1):28-33.
[35]严智强,于书庄.循经感传与经穴发光变化之间关系的探讨[J].中医杂志,1980,30(8):55-58.
[36]池旭生,庞大本,李玉梅,等.人体体表超弱光发射的测量[J].生物物理学报,1994,10(1):165-169.
[37]PARK S H,KIM J,KOO T H.Magneto-acupuncture stimuli effects on ultraweak photon emission from hands of healthy persons[J].J Acu-Merid Stud,2009,2(1):40-48.
[38]YANG J M,CHOI C,HYUM-HEE,et al.Left-right and Yin-Yang balance of biophoton emission from hands[J].Acup Electro-therap Res,2004,29(3/4):197.
[39]韩吟华,丁光宏,沈雪勇,等.人体体表穴位点红外辐射光谱特征及其与ATP能量代谢的关系[J].生物医学工程学进展,2005,26(4):198-200.
[40]李永明.关于肥大细胞与经穴现象的“宋氏理论”及研究方向[J].中国针灸,2016,36(10):1063-1068.
[41]何伟,吴美玲,景向红,等.穴位的本态:穴位组织细胞化学的动态变化[J].中国针灸,2015,35(11):1181-1186.
[42]XU J,ZHENG S,PAN X,et al.The existence of propagated sensation along the meridian proved by neuroelectrophysiology[J].Neural Regen Res,2013,8(28):2633-2640.
[43]ZHANG W B,ZHAO Y,KJELL F,et al.Understanding propagated sensation along meridians by volume transmission in peripheral tissue[J].Chin J Integr Med,2013,19(5):330.
[44]李明伟,王频.人体经穴电学特性研究[J].中医药临床杂志,2015,27(10):1392-1394.
[45]佘延芬,齐丛会,朱江.国内外穴位电学特性研究的历史及进展评述[J].中国针灸,2010,30(12):1047-1050.
[46]STANCIULESCU R B T D.Light in shaping life[J].Homoeopathic Links,2015,28(1):64-65.