微电解水用于SPF级实验动物饮用水初探
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摘要
目的对屏障繁育设施中应用微电解无菌水系统的输水管网、水进行细菌监测,并观察饮用微电解无菌水对SPF级SD大鼠血液生化的影响。方法从2015年～2017年对微电解制水系统6个出水点进行跟踪采样检测。21日龄SD大鼠分为2组,每组雌雄各20只,分别饮用微电解水、高压灭菌水,饮用1～8个月后分别麻醉,腹主动脉取血,检测血液生化。结果对微电解制水系统长达2年多的跟踪检测,微电解水、输水管网均未检测到细菌。21日龄SD大鼠分别饮用微电解水与饮用高压灭菌水1~8月后检测,血液生化指标差异无统计学意义。结论在屏障繁育设施应用微电解无菌水系统,对水及管网有持续性消毒杀菌作用,水和管网长期保持无菌; 21日龄SD大鼠饮用微电解水8个月对血液生化指标没有明显影响。
Objective To monitor the bacteria in the pipe network and the water used in microbiological sterile water system in barrier breeding facilities, and to observe the effect of drinking microelectrolysis sterile water on blood biochemical in SPF-grade SD rats. Methods From the period of 2015 to 2017, 6 outlet points of the micro-electrolysis water system were sampled and detected; 21-day-old SD 20 rats were divided into 2 groups, half male and half female, drinking micro-electrolyzed water and high pressure water respectively. After drinking Sterilized water 1 to 8 months, Blood was taken from the abdominal aorta to detect blood biochemical. Results The microelectrolytic water system has been detected for more than 2 years. Using the current detection technology, no bacteria have been detected in the micro-electrolyzed water and the pipe network. There was no difference in blood biochemical indicators after drinking 1 to 8 months of water in 2 groups. Conclusion The application of micro-electrolysis sterile water system in barrier breeding facilities has a continuous disinfection and bactericidal effect on the pipe network and the water, and can kept sterile for a long time. No effect on blood biochemical indicators were observed in 21-day-old SD rats drinking micro-electrolyzed water for 8 months.
Objective To monitor the bacteria in the pipe network and the water used in microbiological sterile water system in barrier breeding facilities, and to observe the effect of drinking microelectrolysis sterile water on blood biochemical in SPF-grade SD rats. Methods From the period of 2015 to 2017, 6 outlet points of the micro-electrolysis water system were sampled and detected; 21-day-old SD 20 rats were divided into 2 groups, half male and half female, drinking micro-electrolyzed water and high pressure water respectively. After drinking Sterilized water 1 to 8 months, Blood was taken from the abdominal aorta to detect blood biochemical. Results The microelectrolytic water system has been detected for more than 2 years. Using the current detection technology, no bacteria have been detected in the micro-electrolyzed water and the pipe network. There was no difference in blood biochemical indicators after drinking 1 to 8 months of water in 2 groups. Conclusion The application of micro-electrolysis sterile water system in barrier breeding facilities has a continuous disinfection and bactericidal effect on the pipe network and the water, and can kept sterile for a long time. No effect on blood biochemical indicators were observed in 21-day-old SD rats drinking micro-electrolyzed water for 8 months.
引文
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[2]张德胜,高廷耀,李国建,等.微电解水处理的实验研究[J].中国给水排水,1998,14(3):5-7.
[3]赵鹏志.新型管道直饮水管网杀菌器[J].给水排水,2008,34(增刊):287-288.
[4]李兵,陈永昌,王明莲,等微电解灭活大肠杆菌的实验研究[J].净水技术,2007,34:8-10.
[5]王雪峰,李建宏,马宇翔,等.微电解水处理器的杀菌作用研究[J].给水排水,2001,27(11):40-42
[6]朱晓帆,赵修贤,曾抗美,等.饮用水的微电解法灭菌研究[J].四川大学学报:工程科学版,2000,32(1):112-114
[7]王永华,袁伟,舒宏新.饮水中挥发性卤代烃研究[J].环境科学学报,1994,11(2):244-250.
[8]Patermaraxis G,Fountounidis E.Disinfection of water by electrochemical treatment[J].Water Res,1990,24(12):1491-1496.