延胡索中重金属及有害元素的测定
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摘要
本试验应用电热板湿法消解法处理样品,采用电感耦合等离子体-质谱(ICP-MS)法测定延胡索中铜(Cu)、镍(Ni)、铬(Cr)、铅(Pb)、镉(Cd)、锡(Sn)、钴(Co)、汞(Hg)、砷(As)9种重金属及有害元素含量。测定结果表明,9种重金属及有害元素的线性关系良好(r≥0.999 8),检测限为0.001 6～0.022 7μg/g,加样回收率为96.0%～100.4%,RSD≤3.25%;延胡索中Cu、As、Pb、Hg、Cd的含量均低于国家标准《药用植物及制剂进出口绿色行业标准》和《中国药典》2015年版的限量值。所建立的ICP-MS方法具有良好的精密度、重复性和准确性。本试验从有害元素的角度为延胡索在临床应用的安全性评估及建立其质量安全控制标准等提供科学依据。
The samples were treated by hot plate wet digestion method, and nine heavy metals and harmful elements such as Cu,Ni,Cr,Pb,Cd,Sn,Co, Hg and As were determined by inductively coupled plasma-mass spectrometry(ICP-MS). The results show that the linear relationship between the nine heavy metals and harmful elements is good(r≥0.999 8), the detection limit is 0.001 6-0.022 7μg/g, and the recovery rate is 96.0%-100.4%, RSD≤3.25%, the content of Cu, As, Pb, Hg and Cd in Corydalis is lower than the national standard( "Green Industry Standard for Import and Export of Medicinal Plants and Preparations" and "Chinese Pharmacopoeia" 2015 Edition);The ICP-MS method has good precision, repeatability and accuracy. This test provides a scientific basis for the safety evaluation of the clinical application and the establishment of its quality and safety control standards from the perspective of harmful elements.
The samples were treated by hot plate wet digestion method, and nine heavy metals and harmful elements such as Cu,Ni,Cr,Pb,Cd,Sn,Co, Hg and As were determined by inductively coupled plasma-mass spectrometry(ICP-MS). The results show that the linear relationship between the nine heavy metals and harmful elements is good(r≥0.999 8), the detection limit is 0.001 6-0.022 7μg/g, and the recovery rate is 96.0%-100.4%, RSD≤3.25%, the content of Cu, As, Pb, Hg and Cd in Corydalis is lower than the national standard( "Green Industry Standard for Import and Export of Medicinal Plants and Preparations" and "Chinese Pharmacopoeia" 2015 Edition);The ICP-MS method has good precision, repeatability and accuracy. This test provides a scientific basis for the safety evaluation of the clinical application and the establishment of its quality and safety control standards from the perspective of harmful elements.
引文
[1]杜巍娟.延胡索提取物在小鼠体内的药代动力学研究[D].杭州:浙江大学, 2017.
[2]陈东东,王起文,李祥,等.延胡索中1个新生物碱[J].中草药,2016,47(12):2084-2088.
[3]余平,岳显可,顾超,等.延胡索不同部位化学成分及指纹图谱比较分析[J].中华中医药学刊,2017,(6):1435-1438.
[4]石俊敏,韩伟立,叶文才,等.延胡索的化学成分研究[J].天然产物研究与开发,2011,23(4):647-651.
[5]张晓丽,曲扬,侯家鸣,等.延胡索的化学成分[J].沈阳药科大学学报,2008,25(7):537-540.
[6]赵丽沙,董宇,寿旦.延胡索生物碱类化学成分及质量控制研究进展[J].中华中医药学刊,2017,(2):299-302.
[7]李荣,蔡青青,牛彦兵,等.生、熟延胡索饮片药理作用的对比研究[J].中国实验方剂学杂志,2014,20(19):133-137.
[8]胡甜甜,张雪,马世中,等.延胡索中的生物碱成分[J].中国中药杂志,2009,34(15):1917-1920.
[9]王欢,毕福钧,林彤,等.延胡索HPLC指纹图谱研究及9种生物碱含量测定[J].中药材,2017,40(3):624-629.
[10]鲁春梅,张春森,姜立勇.延胡索化学成分及药理作用研究进展[J].中国现代药物应用,2011,5(15):126-127.
[11]刘嘉,蔡小军,狄留庆.延胡索全碱注射液对麻醉犬血流动力学的影响[J].中国现代药物应用,2008, 2(7):6-8.。
[12]程星烨,石钺,郑顺亮,等.延胡索抗心肌缺血有效部位化学成分研究[J].中药材,2008,31(11):1656-1658.
[13]贺凯,高建莉,赵光树.延胡索化学成分、药理作用及质量控制研究进展[J].中草药,2007,38(12):1909-1912.
[14]张国铎.活性指导下中药独活与延胡索抗肿瘤有效成分的分离及其抑瘤作用研究[D].南京:南京中医药大学,2009.
[15]白雪,肖海涛,杨杰,等.延胡索总生物碱对小鼠抗应激能力的研究[J].贵阳医学院学报,2008,33(2):139-140.
[16]王红,田明,王淼,等.延胡索现代药理及临床研究进展[J].中医药学报,2010,38(6):108-111.
[17]Zhu M,Jiang Y,Cui B,et al.Determination of the Heavy Metal Levels in and the Implications for Human Health Risk Assessment[J].Human&Ecological Risk Assessment An International Journal,2015, 21(5):1218-1229.
[18]邹亮,周浓,杨颖,等.不同产地滇重楼中重金属含量的湿法消解-原子荧光光度法测定[J].时珍国医国药, 2010, 21(4):1014-1015.
[19]WM2-2001,药用植物及制剂进出口绿色行业标准[S].
[2]陈东东,王起文,李祥,等.延胡索中1个新生物碱[J].中草药,2016,47(12):2084-2088.
[3]余平,岳显可,顾超,等.延胡索不同部位化学成分及指纹图谱比较分析[J].中华中医药学刊,2017,(6):1435-1438.
[4]石俊敏,韩伟立,叶文才,等.延胡索的化学成分研究[J].天然产物研究与开发,2011,23(4):647-651.
[5]张晓丽,曲扬,侯家鸣,等.延胡索的化学成分[J].沈阳药科大学学报,2008,25(7):537-540.
[6]赵丽沙,董宇,寿旦.延胡索生物碱类化学成分及质量控制研究进展[J].中华中医药学刊,2017,(2):299-302.
[7]李荣,蔡青青,牛彦兵,等.生、熟延胡索饮片药理作用的对比研究[J].中国实验方剂学杂志,2014,20(19):133-137.
[8]胡甜甜,张雪,马世中,等.延胡索中的生物碱成分[J].中国中药杂志,2009,34(15):1917-1920.
[9]王欢,毕福钧,林彤,等.延胡索HPLC指纹图谱研究及9种生物碱含量测定[J].中药材,2017,40(3):624-629.
[10]鲁春梅,张春森,姜立勇.延胡索化学成分及药理作用研究进展[J].中国现代药物应用,2011,5(15):126-127.
[11]刘嘉,蔡小军,狄留庆.延胡索全碱注射液对麻醉犬血流动力学的影响[J].中国现代药物应用,2008, 2(7):6-8.。
[12]程星烨,石钺,郑顺亮,等.延胡索抗心肌缺血有效部位化学成分研究[J].中药材,2008,31(11):1656-1658.
[13]贺凯,高建莉,赵光树.延胡索化学成分、药理作用及质量控制研究进展[J].中草药,2007,38(12):1909-1912.
[14]张国铎.活性指导下中药独活与延胡索抗肿瘤有效成分的分离及其抑瘤作用研究[D].南京:南京中医药大学,2009.
[15]白雪,肖海涛,杨杰,等.延胡索总生物碱对小鼠抗应激能力的研究[J].贵阳医学院学报,2008,33(2):139-140.
[16]王红,田明,王淼,等.延胡索现代药理及临床研究进展[J].中医药学报,2010,38(6):108-111.
[17]Zhu M,Jiang Y,Cui B,et al.Determination of the Heavy Metal Levels in and the Implications for Human Health Risk Assessment[J].Human&Ecological Risk Assessment An International Journal,2015, 21(5):1218-1229.
[18]邹亮,周浓,杨颖,等.不同产地滇重楼中重金属含量的湿法消解-原子荧光光度法测定[J].时珍国医国药, 2010, 21(4):1014-1015.
[19]WM2-2001,药用植物及制剂进出口绿色行业标准[S].