低温胁迫对4个菜用甘薯品种生理生化特性的影响
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摘要
为探索菜用甘薯抗寒机理,以‘宁菜3号’、‘CH-2’、‘福薯18’、‘福薯10号’4个菜用甘薯品种为试验材料,采用温室越冬和人工气候室模拟低温胁迫的方法,对菜用甘薯抗寒性与POD、SOD活性及可溶性蛋白质含量间的关系进行了研究。结果表明:受低温胁迫后,‘宁菜3号’、‘CH-2’的POD活性增加速度快、活性强、酶活力持久,SOD活性高,SOD活性及可溶性蛋白质含量下降幅度相对较小。在低温胁迫后,POD、SOD活性变化及可溶性蛋白质含量的不同可能是引起菜用甘薯抗寒性差异的原因之一。本研究对菜用甘薯栽培的品种选择具有一定的指导意义,为进一步探索菜用甘薯抗寒机理提供参考。
In order to explore the cold resistance mechanism of vegetable sweet potato,four sweet potato varieties('Ningcai No.3','CH-2','Fukushu 18' and 'Fushu 10') were used as experimental materials.Meanwhile,low-temperature stress was simulated through greenhouse wintering and artificial climate chamber.Thus,the relationship between cold resistance and POD,SOD activity,soluble protein content in vegetable sweet potato was studied.The results showed that,under low temperature stress,the POD activity of 'Ningcai No.3','CH-2' increased rapidly,and its activity was strong,but the decline of SOD activity and soluble protein content was relatively small and SOD activity was high.The change of POD,SOD activity and the difference of the soluble protein content might be one of the reasons for the difference of cold resistance of vegetable sweet potato after low temperature stress.There might have a guiding significance for the selection of vegetable sweet potatoes in this study,and provided a reference for further exploring of cold resistance mechanism in vegetable sweet potatoes.
In order to explore the cold resistance mechanism of vegetable sweet potato,four sweet potato varieties('Ningcai No.3','CH-2','Fukushu 18' and 'Fushu 10') were used as experimental materials.Meanwhile,low-temperature stress was simulated through greenhouse wintering and artificial climate chamber.Thus,the relationship between cold resistance and POD,SOD activity,soluble protein content in vegetable sweet potato was studied.The results showed that,under low temperature stress,the POD activity of 'Ningcai No.3','CH-2' increased rapidly,and its activity was strong,but the decline of SOD activity and soluble protein content was relatively small and SOD activity was high.The change of POD,SOD activity and the difference of the soluble protein content might be one of the reasons for the difference of cold resistance of vegetable sweet potato after low temperature stress.There might have a guiding significance for the selection of vegetable sweet potatoes in this study,and provided a reference for further exploring of cold resistance mechanism in vegetable sweet potatoes.
引文
He H.Y.,Tian L.P.,and Xue L.,2007,Research progress on physiology and biochemistry of plant cold hardiness,Tianjin Nongye Kexue(Tianjin Agricultural Sciences),13(2):10-13(和红云,田丽萍,薛琳,2007,植物抗寒性生理生化研究进展,天津农业科学,13(2):10-13)
Li J.S.,Geng G.D.,and Cheng Z.H.,2003,Effects of chilling stress on chill-resistance physiological and biochemicalindexes of eggplant seedlings,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)),31(1):90-92(李建设,耿广东,程智慧,2003,低温胁迫对茄子幼苗抗寒性生理生化指标的影响,西北农林科技大学学报(自然科学版),31(1):90-92)
Meng F.Z.,Zhang Z.X.,Yu X.C.,and Ai X.Z.,2005,Effect of low temperature stress on some physiological and biochemical characteristics of Chinese cabbage in field,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of Eco-Agriculture),13(2):84-86(孟凡珍,张振贤,于贤昌,艾希珍,2005,田间低温胁迫对大白菜某些理化特性的影响研究,中国生态农业学报,13(2):84-86)
Wang Y.H.,Yang X.S.,Yao G.X.,Zhu H.Y.,Lei J.,and Su W.J.,2011,Research status and characteristics of sweetpotato for vegetable,Hubei Nongye Keji(Hubei Agricultural Sciences),50(10):2028-2030(望宇洪,杨新笋,姚国新,祝海英,雷剑,苏文瑾,2011,菜用甘薯的特征特性与研究现状,湖北农业科学,50(10):2028-2030)
Wang Y.Z.,Yang Z.G.,Zeng X.Q.,and Zhang T.H.,2010,Effect on the content of proline,SOD and CAT and protein in leaf of artemisia annua at low temperature stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),29(5):927-930(王易振,杨治国,曾祥琼,张廷惠,2010,低温处理对青蒿叶片脯氨酸、SOD和CAT的含量及蛋白质的影响,基因组学与应用生物学,29(5):927-930)
Wu Y.J.,and Liu J.H.,2007,Effects of chilling stress on chill resistance physiological and biochemical indexes of muskmelon seedlings,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)),35(3):139-143(武雁军,刘建辉,2007,低温胁迫对厚皮甜瓜幼苗抗寒性生理生化指标的影响,西北农林科技大学学报(自然科学版),35(3):139-143)
Yang N.N.,Sun W.C.,Liu Z.G.,Shi P.H.,Fang Y.,Wu J.Y.,Zeng X.C.,Kong D.J.,Lu M.H.,and Wang Y.,2014,Morphological characters and physiological mechanisms of cold resistance of winter rapeseed in northern China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(3):452-461(杨宁宁,孙万仓,刘自刚,史鹏辉,方彦,武军艳,曾秀存,孔德晶,鲁美宏,王月,2014,北方冬油菜抗寒性的形态与生理机制,中国农业科学,47(3):452-461)
Zhang X.B.,Zhu G.P.,Nan W.Z.,Zhan L.L.,and Teng S.M.,2016,Evaluation of cold tolerance of five sweet potato varieties by membership function method,Redai Shengwu Xuebao(Journal of Tropical Biology),7(4):450-456(张小贝,朱国鹏,南文卓,占雷雷,滕少梅,2016,隶属函数法对5种菜用甘薯耐寒性的评价,热带生物学报,7(4):450-456)
Zhang X.B.,Zhu Z.X.,Nan W.Z.,Sun Y.B.,and Zhu G.P.,2017,Effects of 2,4-epibrassinolide(EBR)on cold resistance-related physiological-biochemical indexes of vegetable sweet potato,Anhui Nongye Daxue Xuebao(Journal of Anhui A-gricultural University),44(3):525-529(张小贝,祝志欣,南文卓,孙言博,朱国鹏,2017,2,4-表油菜素内酯(EBR)对菜用甘薯抗寒生理生化的影响,安徽农业大学学报,44(3):525-529)
Zhou H.,Zhang C.F.,and Huang Y.F.,2009,Development and utilization of sweet potato variety for vegetable use with shoot tip,Zuowu Yanjiu(Crop Research),23(3):220-224(周虹,张超凡,黄艳岚,2009,茎尖菜用甘薯品种的开发与利用,作物研究,23(3):220-224)
Zhu H.X.,Sun W.C.,Deng B.,Yan N.,Wu J.Y.,Fan H.L.,Ye J.,Zeng J.,Liu Y.L.,and Zhang Y.H.,2007,Study on cold hardiness and its physiological and biochemical characteristics of winter turnip rape(Brassica campetris),Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),16(4):34-38(朱惠霞,孙万仓,邓斌,燕妮,武军艳,范惠玲,叶剑,曾军,刘雅丽,张亚宏,2007,白菜型冬油菜品种的抗寒性及其生理生化特性,西北农业学报,16(4):34-38)
Li J.S.,Geng G.D.,and Cheng Z.H.,2003,Effects of chilling stress on chill-resistance physiological and biochemicalindexes of eggplant seedlings,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)),31(1):90-92(李建设,耿广东,程智慧,2003,低温胁迫对茄子幼苗抗寒性生理生化指标的影响,西北农林科技大学学报(自然科学版),31(1):90-92)
Meng F.Z.,Zhang Z.X.,Yu X.C.,and Ai X.Z.,2005,Effect of low temperature stress on some physiological and biochemical characteristics of Chinese cabbage in field,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of Eco-Agriculture),13(2):84-86(孟凡珍,张振贤,于贤昌,艾希珍,2005,田间低温胁迫对大白菜某些理化特性的影响研究,中国生态农业学报,13(2):84-86)
Wang Y.H.,Yang X.S.,Yao G.X.,Zhu H.Y.,Lei J.,and Su W.J.,2011,Research status and characteristics of sweetpotato for vegetable,Hubei Nongye Keji(Hubei Agricultural Sciences),50(10):2028-2030(望宇洪,杨新笋,姚国新,祝海英,雷剑,苏文瑾,2011,菜用甘薯的特征特性与研究现状,湖北农业科学,50(10):2028-2030)
Wang Y.Z.,Yang Z.G.,Zeng X.Q.,and Zhang T.H.,2010,Effect on the content of proline,SOD and CAT and protein in leaf of artemisia annua at low temperature stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),29(5):927-930(王易振,杨治国,曾祥琼,张廷惠,2010,低温处理对青蒿叶片脯氨酸、SOD和CAT的含量及蛋白质的影响,基因组学与应用生物学,29(5):927-930)
Wu Y.J.,and Liu J.H.,2007,Effects of chilling stress on chill resistance physiological and biochemical indexes of muskmelon seedlings,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)),35(3):139-143(武雁军,刘建辉,2007,低温胁迫对厚皮甜瓜幼苗抗寒性生理生化指标的影响,西北农林科技大学学报(自然科学版),35(3):139-143)
Yang N.N.,Sun W.C.,Liu Z.G.,Shi P.H.,Fang Y.,Wu J.Y.,Zeng X.C.,Kong D.J.,Lu M.H.,and Wang Y.,2014,Morphological characters and physiological mechanisms of cold resistance of winter rapeseed in northern China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(3):452-461(杨宁宁,孙万仓,刘自刚,史鹏辉,方彦,武军艳,曾秀存,孔德晶,鲁美宏,王月,2014,北方冬油菜抗寒性的形态与生理机制,中国农业科学,47(3):452-461)
Zhang X.B.,Zhu G.P.,Nan W.Z.,Zhan L.L.,and Teng S.M.,2016,Evaluation of cold tolerance of five sweet potato varieties by membership function method,Redai Shengwu Xuebao(Journal of Tropical Biology),7(4):450-456(张小贝,朱国鹏,南文卓,占雷雷,滕少梅,2016,隶属函数法对5种菜用甘薯耐寒性的评价,热带生物学报,7(4):450-456)
Zhang X.B.,Zhu Z.X.,Nan W.Z.,Sun Y.B.,and Zhu G.P.,2017,Effects of 2,4-epibrassinolide(EBR)on cold resistance-related physiological-biochemical indexes of vegetable sweet potato,Anhui Nongye Daxue Xuebao(Journal of Anhui A-gricultural University),44(3):525-529(张小贝,祝志欣,南文卓,孙言博,朱国鹏,2017,2,4-表油菜素内酯(EBR)对菜用甘薯抗寒生理生化的影响,安徽农业大学学报,44(3):525-529)
Zhou H.,Zhang C.F.,and Huang Y.F.,2009,Development and utilization of sweet potato variety for vegetable use with shoot tip,Zuowu Yanjiu(Crop Research),23(3):220-224(周虹,张超凡,黄艳岚,2009,茎尖菜用甘薯品种的开发与利用,作物研究,23(3):220-224)
Zhu H.X.,Sun W.C.,Deng B.,Yan N.,Wu J.Y.,Fan H.L.,Ye J.,Zeng J.,Liu Y.L.,and Zhang Y.H.,2007,Study on cold hardiness and its physiological and biochemical characteristics of winter turnip rape(Brassica campetris),Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),16(4):34-38(朱惠霞,孙万仓,邓斌,燕妮,武军艳,范惠玲,叶剑,曾军,刘雅丽,张亚宏,2007,白菜型冬油菜品种的抗寒性及其生理生化特性,西北农业学报,16(4):34-38)