不同光质对苦荞芽黄酮类物质及抗氧化活性的影响
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摘要
将苦荞分别在红光(630 nm)、蓝光(460 nm)、白光(540 nm)条件下发芽,研究其芦丁、槲皮素、花青素含量和抗氧化活性的变化。采用高效液相色谱法(HPLC)测定苦荞芽中芦丁、槲皮素、花青素含量,用分光光度法测定苦荞芽提取物对DPPH自由基、ABTS自由基的清除能力。结果表明,光照条件下苦荞芽的生长受到抑制,其中抑制效果为蓝光>白光>红光;在蓝光条件下,苦荞芽中芦丁、槲皮苷和槲皮素含量显著增加,同时其抗氧化活性有所提高;红光和蓝光条件下,苦荞芽中花青素能够有效累积。
The changes of rutin, quercetin, anthocyanin content and antioxidant activity of tartary buckwheat buds under red light(630 nm), blue light(460 nm) and white light(540 nm) were investigated. The content of rutin, quercetin and anthocyanin in tartary buckwheat buds was determined by high performance liquid chromatography(HPLC). The scavenging ability of extracts of tartary buckwheat buds on DPPH free radicals and ABTS free radicals was determined by spectrophotometry. The results showed that the growth of tartary buckwheat buds was inhibited under light conditions, and the inhibitory effect was blue light>white light>red light; the content of rutin, quercetin and quercetin in tartary buckwheat buds increased significantly, as well as the antioxidant activity is improved under blue light conditions; the anthocyanins in the buckwheat bud can be effectively accumulated under the conditions of red light and blue light.
The changes of rutin, quercetin, anthocyanin content and antioxidant activity of tartary buckwheat buds under red light(630 nm), blue light(460 nm) and white light(540 nm) were investigated. The content of rutin, quercetin and anthocyanin in tartary buckwheat buds was determined by high performance liquid chromatography(HPLC). The scavenging ability of extracts of tartary buckwheat buds on DPPH free radicals and ABTS free radicals was determined by spectrophotometry. The results showed that the growth of tartary buckwheat buds was inhibited under light conditions, and the inhibitory effect was blue light>white light>red light; the content of rutin, quercetin and quercetin in tartary buckwheat buds increased significantly, as well as the antioxidant activity is improved under blue light conditions; the anthocyanins in the buckwheat bud can be effectively accumulated under the conditions of red light and blue light.
引文
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[16]孙军涛,任顺,查磊.萌发对荞麦籽粒营养成分的影响研究进展[J].农产品加工(学刊),2013,10(9):46-49.
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[18]赵钢.荞麦的营养与功能[M].北京:科学出版社,2012.
[19]陈志杰,吴嘉琪,马燕,等.植物食品原料中酚酸的生物合成与调控及其生物活性研究进展[J].食品科学,2018,39(7):321-328.
[20]Guo B,Liu YG,Yan Q,et al.Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim[J].Plant Growth Regulation,2007,52:259-263.
[2]马麟,彭镰心,赵钢.我国苦荞芽菜生产及其食品开发研究进展[J].农产品加工(学刊),2015,21:64-67.
[3]殷培蕾,李静,邓园园.光照对苦荞芽多酚类物质及抗氧化活性的影响[J].成都大学学报(自然科学版),2015,34(3):209-213.
[4]Tsurunaga Y,Takahashi T,Katsube T,et al.Effects of UV-B irradiation on the levels of anthocyanin,rutin and radical scavenging activity of buckwheat sprouts[J].Food Chemistry,2013,141(1):552-556.
[5]雒晓鹏,卜星星,赵海霞.LED光源对芽期苦荞黄酮合成的影响[J].食品科学,2015,36(03):86-89.
[6]张采琼,赵江林,赵钢,等.香菇多糖对苦荞萌发及黄酮合成的影响[J].食品工业,2014,(8):123-126.
[7]姚钰蓉.紫甘薯花青素的提取纯化、稳定性及抗氧化活性研究[D].保定:河北农业大学,2009.
[8]赵琳.苦荞萌发期生理活性及其蛋白抗菌性的研究[D].上海:上海师范大学,2012.
[9]张欢,徐志刚,崔瑾.不同光质对萝卜芽苗菜生长和营养品质的影响[J].中国蔬菜,2009,(10):28-32.
[10]娄秋艳.黑米花青素的组成、对益生菌和致病菌的活性研究[D].合肥:合肥工业大学,2017.
[11]胡可,韩科厅,戴思兰.环境因子调控植物花青素苷合成及呈色的机制[J].植物学报,2010,45(3):307-317.
[12]Li X,Thwe A A,Park N I.Accumulation of phenylpropanoids and correlated gene expression during the development of tartary buckwheat sprouts[J].Journal of Agricultural and Food Chemistry,2012,60:5629-5635.
[13]Zhou B,Wang Y,Zhan Y,et al.Chalcone synthase family genes have redundant roles in anthocyanin biosynthesis and in response to blue/UV-A light in turnip(Brassica rapa;Brassicaceae)[J].American Journal of Botany,2013,100(12):2458-2467.
[14]Ohto M,Onai K,Furukawa Y,et al.Effects of sugar on vegetative development and floral transition in Arabidopsis[J].Plant Physiology,2001,127(1):252-261.
[15]张泽岑,王能彬.光质对茶树花青素含量的影响[J].四川农业大学学报,2002,20(4):337-382.
[16]孙军涛,任顺,查磊.萌发对荞麦籽粒营养成分的影响研究进展[J].农产品加工(学刊),2013,10(9):46-49.
[17]任顺成,孙军涛.荞麦粉皮芽中黄酮类化合物抗氧化研究[J].河南工业大学学报(自然科学版),2008,29(2):15-17.
[18]赵钢.荞麦的营养与功能[M].北京:科学出版社,2012.
[19]陈志杰,吴嘉琪,马燕,等.植物食品原料中酚酸的生物合成与调控及其生物活性研究进展[J].食品科学,2018,39(7):321-328.
[20]Guo B,Liu YG,Yan Q,et al.Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim[J].Plant Growth Regulation,2007,52:259-263.