微波超声波组合提取猴头菇多糖工艺优化及其抗氧化活性
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摘要
通过单因素试验分别考察粉碎粒度、料液体积质量比、提取温度、提取时间、微波功率和超声波功率对猴头菇多糖提取得率的影响,确定各因素的适宜水平。在单因素试验基础上,应用Box-Behnken试验设计和响应面分析法,探讨料液体积质量比、提取温度、提取时间和超声波功率对提取猴头菇多糖得率的影响。响应面优化结果表明,微波超声波组合提取猴头菇多糖的最优工艺为:粉碎粒度20目、液料体积质量比20 mL/g、提取温度74℃、提取时间16 min、微波功率200 W、超声波功率1 052 W。在最优工艺条件下,多糖得率为6.44%,非常接近预测值,说明所以优化的提取工艺参数可靠。体外抗氧化活性结果表明,微波超声波组合提取的猴头菇多糖抗氧化活性较高,对羟基自由基、DPPH自由基和超氧阴离子自由基清除作用显著,可以作为一种良好的天然抗氧化剂。
A series of single-factor experiments were made to investigate the effects of particle size,liquid-to-solid ratio,extraction temperature,extraction time,microwave power,ultrasonic power. On the basis of single factor investigation,response surface methodology(RSM) based on Box-Behnken experimental design was used to explore the effect of liquid-to-solid ratio,extraction temperature,extraction time and ultrasonic power on extraction efficiency.The optimum process parameters for an integrated microwave and ultrasonic reaction system extraction process were obtained as:particle size 20,the ratio of water to material 20 mL/g,extraction temperature 74 ℃,extraction time 16 min,microwave power 200 W,ultrasonic power 1 052 W,6.44% of the yield of polysaccharide was achieved. The predicted values for optimization process conditions were in good agreement with experimental data,indicating that the optimum process parameters by RMS were reliable. The Hericium erinaceus polysaccharides showed potential scavenging activity against hydroxyl hydroxyl,superoxide anionradical and DPPH. This study indicates that Hericium erinaceus polysaccharides are a good source of natural antioxidants.
A series of single-factor experiments were made to investigate the effects of particle size,liquid-to-solid ratio,extraction temperature,extraction time,microwave power,ultrasonic power. On the basis of single factor investigation,response surface methodology(RSM) based on Box-Behnken experimental design was used to explore the effect of liquid-to-solid ratio,extraction temperature,extraction time and ultrasonic power on extraction efficiency.The optimum process parameters for an integrated microwave and ultrasonic reaction system extraction process were obtained as:particle size 20,the ratio of water to material 20 mL/g,extraction temperature 74 ℃,extraction time 16 min,microwave power 200 W,ultrasonic power 1 052 W,6.44% of the yield of polysaccharide was achieved. The predicted values for optimization process conditions were in good agreement with experimental data,indicating that the optimum process parameters by RMS were reliable. The Hericium erinaceus polysaccharides showed potential scavenging activity against hydroxyl hydroxyl,superoxide anionradical and DPPH. This study indicates that Hericium erinaceus polysaccharides are a good source of natural antioxidants.
引文
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[4]BYUNG K Y,JUN B P,CHI H S.Hypolipidemic effect of an ex-o-biopolymer produced from a submerged mycelial culture of Heri-cium erinaceum[J].Bioscience Biotechnology and Biochemistry,2003,67(6):1292-1298.
[5]KIM D M,PYUN C W,KO H G.Isolation of antimicrobial sub-stances from Hericiumerinaceum[J].Mycobiology,2000,28:33-38.
[6]LIU Rixin.Research and utilization of edible[J].Edible Fungi of China,1998(4):5-7.(in Chinese)
[7]MIAO Xiaoyan,CHEN Ping,CUI Jue,et al.Microwave-assisted enzymatic extraction of polysaccharides from cultured mycelia of Hericium erinaceus[J].Food Science,2012(2):94-97.(in Chinese)
[8]TENG Jingtong,ZHAN Xiaolong,GONG Kun et al.Optimization of ultrasonic-microwave synergistic extraction of total flavonoids from leaves of Punica grnatum L.by response surface methodology[J].Journal of Food Science and Biotechnology,2015(7):744-750.(in Chinese)
[9]XU Guangyu,YAN Jun,GUO Xiaoqiang,et al.The betterment and apply of phenol-sulphate acid method[J].Food Science,2005(8):342-346.(in Chinese)
[10]王福荣.酿酒分析与检测[M].北京:化学工业出版社,2005.
[11]LUO A X,HE X J,ZHOU S D,et al.Purification,composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl[J].Carbohydrate Polymers,2010,79(4):1014-1019.
[12]QIAO D L,KE C L,HU B,et al.Antioxidant activities of polysaccharides from Hyriopsis cumingii[J].Carbohydrate Polymers,2009,78:199-204.
[13]XU Yajuan,ZHAO Yanjing,HU Hong.Research on the measurement of the SOD activity via pyrogallol auto-oxidation[J].Journal of Southwest University for Nationalities:Natural Science Edition,2006,32(6):1207-1209.(in Chinese)
[14]ZHANG Changjun,YUAN Fangyuan,SHAO Hongbing.Application of extracting polysaccharide compound by ultrasonic method[J].Chemical Industry Times,2007,21(2):54-56.(in Chinese)
[15]ZHANG Daijia,LIU Chuanbin,XIU Zhilong,et al.Application of microwave technique in extraction of effective components from plant[J].Chinese Traditional and Herbal Drugs,2000,31(9):5-6.(in Chinese)
[16]ZHU Fangming,MA Xin,ZHANG Anqiang,et al.Optimization of ultrasound-assisted extraction of Radix Glehniae polysaccharides using response surface methology[J].Food Science Aand Technology,2011,36(7):153-156.(in Chinese)
[17]刘四光.自养小球藻(Chlorella autotrophica)多糖提取,纯化,化学结构及抗菌活性研究[D].厦门:厦门大学,2007.
[18]XU H,SUN L,SHI Y,et al.Optimization of cultivation conditions for extracellular polysaccharide and mycelium biomass byMorchella esculenta As51620[J].Biochemical Engineering Journal,2008,39(1):66-73.
[19]WAN Jun,JIANG Aimin,QU Zhi,et al.Optimization of processing conditions for camembert cheese by response surface method[J].Modern Food Science and Technology,2012,28(7):840-844.(in Chinese)