超声波辅助提取金线草黄酮工艺研究
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摘要
以金线草为原料,黄酮类化合物提取率为指标,研究了超声波辅助法提取黄酮类化合物的最佳工艺条件。通过单因素试验研究了液料比、乙醇浓度、超声波处理时间3个因素对提取效果的影响,并利用响应面试验设计对提取工艺进行优化,得到最佳工艺条件为液料比23∶1(m L/g)、乙醇浓度75%、超声波处理时间11 min,在此条件下黄酮类化合物的实际提取率为2.576%。
With Rubia membranacea as raw material, the optimum ultrasound-assisted extraction conditions of flavonoids were studied, using extraction rate of flavonoids as a index. The effects of liquid to material ratio, ethanol concentration and ultrasonic treatment time on the extraction efficiency were studied by single factor experiment, and the extraction process was optimized by response surface design. The optimum conditions were obtained as follows: liquid to material ratio 23 ∶ 1(mL/g), ethanol concentration 75 %, ultrasonic treatment time 11 min. Under these conditions, the actual extraction rate of flavonoids was 2.576 %.
With Rubia membranacea as raw material, the optimum ultrasound-assisted extraction conditions of flavonoids were studied, using extraction rate of flavonoids as a index. The effects of liquid to material ratio, ethanol concentration and ultrasonic treatment time on the extraction efficiency were studied by single factor experiment, and the extraction process was optimized by response surface design. The optimum conditions were obtained as follows: liquid to material ratio 23 ∶ 1(mL/g), ethanol concentration 75 %, ultrasonic treatment time 11 min. Under these conditions, the actual extraction rate of flavonoids was 2.576 %.
引文
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[11]高波,胡洪瑞,李河,等.超声波辅助提取刺芫荽黄酮条件的优化[J].江苏农业科学, 2017, 45(6):175-177.
[2]常徽,糜漫天.植物黄酮抗肿瘤研究进展[J].环境卫生学杂志,2006, 33(5):296-300.
[3]周晓丽,邵震,李婷婷,等.木瓜黄酮的提取及抗氧化性研究[J].食品工业科技, 2007, 28(8):170-172.
[4]李红军.银杏叶中黄酮类化合物的提取及其体外抗氧化活性研究[J].北方园艺, 2013(5):156-159.
[5]李湘洲,栾芳菲,张胜,等.黄酮类化合物的抗肿瘤和抗血管生成作用研究进展[J].食品与机械, 2016, 32(1):199-201.
[6]徐春华,张治广,谢明杰.大豆异黄酮的抗氧化和抗肿瘤活性研究[J].大豆科学, 2010, 29(5):870-873.
[7]万巧凤,吴莉,杨美玲,等.槲皮素对甲型H1N1流感病毒诱导的A549细胞凋亡效应酶Caspase-3的影响[J].中国中医药信息杂志, 2011, 18(10):42-44.
[8]高波,胡洪瑞,李河,等.超声波辅助提取刺芫荽黄酮条件的优化[J].江苏农业科学, 2017, 45(6):175-177.
[9]刘珊,胡洪利,陈惠.响应面法优化金龙胆草总黄酮的超声波提取工艺研究[J].河南农业科学, 2013, 42(1):148-151, 159.
[10]王蕙,赵树全,吴凌,等.正交实验法优选银杏叶总黄酮的半仿生提取工艺[J].食品科技, 2009, 34(2):207-209.
[11]高波,胡洪瑞,李河,等.超声波辅助提取刺芫荽黄酮条件的优化[J].江苏农业科学, 2017, 45(6):175-177.