人工老化对小麦种子活力及超弱发光的影响
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摘要
为阐明人工老化对种子超弱发光(UWL)的影响及UWL与种子活力的相关性,探索一种种子活力无损检测新方法,以小麦种子‘济麦22’为材料,研究了人工老化过程中超弱发光的变化及其与种子活力、ATP含量的关系。研究结果显示:随着老化时间的延长,种子的发芽率、发芽势、发芽指数、UWL强度和ATP含量均逐渐下降;人工老化48 h后,与对照相比分别降低了19.19%、34.34%、38.78%、27.77%和13.84%。相关分析表明,UWL强度与发芽率、发芽势、发芽指数和ATP含量显著正相关。因此UWL作为一种快速、无损、灵敏的检测方法可应用于种子活力无损检测研究。
In order to ascertain the effect of artificial aging on ultraweak luminescence(UWL) and thecorrelation between UWL and seed vigor, and explore a new way of seed vigor non-destructive testing, seeds ofwheat variety‘Jimai 22'were used to study the change of UWL intensity and its relationship to seed vigor andATP content during artificial aging. The results showed that with the extension of artificial aging time, thegermination percentage, germination potential, germination index, UWL intensity and ATP content decreasedgradually; after artificial aging for 48 h, they were reduced by 19.19%, 34.34%, 38.78%, 27.77% and 13.84%,respectively, compared with CK. Correlation analysis indicated that the UWL intensity was significantlypositive correlated with germination percentage, germination potential, germination index and ATP content. Itwas concluded that UWL as a fast, non-destructive and sensitive detection method could be applied in thestudy of seed vigor non-destructive testing.
In order to ascertain the effect of artificial aging on ultraweak luminescence(UWL) and thecorrelation between UWL and seed vigor, and explore a new way of seed vigor non-destructive testing, seeds ofwheat variety‘Jimai 22'were used to study the change of UWL intensity and its relationship to seed vigor andATP content during artificial aging. The results showed that with the extension of artificial aging time, thegermination percentage, germination potential, germination index, UWL intensity and ATP content decreasedgradually; after artificial aging for 48 h, they were reduced by 19.19%, 34.34%, 38.78%, 27.77% and 13.84%,respectively, compared with CK. Correlation analysis indicated that the UWL intensity was significantlypositive correlated with germination percentage, germination potential, germination index and ATP content. Itwas concluded that UWL as a fast, non-destructive and sensitive detection method could be applied in thestudy of seed vigor non-destructive testing.
引文
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[2]张新华,杨洪强.植物的超微弱发光[J].山东农业大学学报:自然科学版,2003,34(4):605-608.
[3]Popp F A.Biophotonemmission[J].Experientia,1988,44(7):543-544.
[4]Hideg E,Bjorn L O.Ultraweak light emission,free radicals,chilling and light sensitivity[J].Physiol Plant,1996,98:223-228.
[5]Slawlnski J.Luminescence research and its relation to ultraweak cell radiation[J].Experiential,1988,33(7):559-571.
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[8]谭石慈,邢达,唐永红,等.植物叶片超微弱发光光谱研究[J].光子学报,2000,29(11):961-965.
[9]张新华,李富军,申琳,等.超弱发光技术在植物逆境生理研究中的应用[J].植物生理学通讯,2009,45(9):931-935.
[10]Yan Y,Popp F A,Rothe G M.Correlation between germination capacity and biophoton emission of barley seeds(Hordeumvulgare L.)[J].Seed Sci Technol,2003,31(2):249-258.
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[12]张新华,杨洪强,李富军.水分胁迫下苹果幼苗超弱发光及一些生理特性的变化[J].西北植物学报,2004,24(4):720-724.
[13]袁佐清.生物超微弱发光研究进展[J].安徽农业科学,2008,36(8):3092-3094.
[14]张菊平,张兴志,巩振辉.超微弱发光在蔬菜研究中的应用[J].中国农学通报,2006,22(1):220-222.
[15]李富军,张新华.微弱发光技术在农产品检测中的应用研究进展[J].农业工程学报,2006,22(12),260-264.
[16]国家技术监督局.中华人民共和国国家标准:农作物种子检验规程(GB/T3543.3)[M].北京:中国标准出版社,1995.
[17]王维光,顾俭本.从叶片中提取ATP方法的比较[J].植物生理学通讯,1986(5):54-55.
[18]鲍杰,吴才章.基于虚拟仪器的小麦活力检测[J].中国粮油学报,2011,26(6):102-105.
[19]吴才章,徐振方,王继伟.小麦发光特性研究[J].中国粮油学报,2014,29(4):78-81.
[20]郑亚飞.两种蔬菜种子生物超弱发光与活力相关性的研究[J].德州学院学报,2010,26(6):5-7.
[21]张文兰,田茜,李群,等.低温保存对4种作物种子超弱发光特性影响规律的研究[J].山东农业科学,2014,46(12):38-42.
[22]国颖,杨洪强.高温对平邑甜茶幼苗生物发光与能量代谢的影响[J].园艺学报,2008,35(1):99-102.
[23]林桂玉,黄在范,张翠华,等.菊花花芽分化期超微弱发光及其生理代谢的变化[J].园艺学报,2008,35(12):1819-1824.
[24]张新华,杨洪强,李富军,等.杏花开放过程中超微弱发光和ATP及活性氧含量的变化[J].植物生理与分子生物学学报,2004,30(1):41-44.
[2]张新华,杨洪强.植物的超微弱发光[J].山东农业大学学报:自然科学版,2003,34(4):605-608.
[3]Popp F A.Biophotonemmission[J].Experientia,1988,44(7):543-544.
[4]Hideg E,Bjorn L O.Ultraweak light emission,free radicals,chilling and light sensitivity[J].Physiol Plant,1996,98:223-228.
[5]Slawlnski J.Luminescence research and its relation to ultraweak cell radiation[J].Experiential,1988,33(7):559-571.
[6]Rattemeyer M R,Popp F A,Nagl W,et al.Evidence of photon emission from DNA in living cells[J].Naturwissenschaften,1981,68:572-573.
[7]毛大璋,沈恂,张月敬,等.代谢抑制剂对萌发绿豆超弱发光的影响[J].生物物理学报,1988,4(2):116-120.
[8]谭石慈,邢达,唐永红,等.植物叶片超微弱发光光谱研究[J].光子学报,2000,29(11):961-965.
[9]张新华,李富军,申琳,等.超弱发光技术在植物逆境生理研究中的应用[J].植物生理学通讯,2009,45(9):931-935.
[10]Yan Y,Popp F A,Rothe G M.Correlation between germination capacity and biophoton emission of barley seeds(Hordeumvulgare L.)[J].Seed Sci Technol,2003,31(2):249-258.
[11]Costanzo E,Gulino M,Lanzano L,et al.Single seed viability checked bydelayedluminescence[J].Euro Biophys J.,2008,37(2):235-238.
[12]张新华,杨洪强,李富军.水分胁迫下苹果幼苗超弱发光及一些生理特性的变化[J].西北植物学报,2004,24(4):720-724.
[13]袁佐清.生物超微弱发光研究进展[J].安徽农业科学,2008,36(8):3092-3094.
[14]张菊平,张兴志,巩振辉.超微弱发光在蔬菜研究中的应用[J].中国农学通报,2006,22(1):220-222.
[15]李富军,张新华.微弱发光技术在农产品检测中的应用研究进展[J].农业工程学报,2006,22(12),260-264.
[16]国家技术监督局.中华人民共和国国家标准:农作物种子检验规程(GB/T3543.3)[M].北京:中国标准出版社,1995.
[17]王维光,顾俭本.从叶片中提取ATP方法的比较[J].植物生理学通讯,1986(5):54-55.
[18]鲍杰,吴才章.基于虚拟仪器的小麦活力检测[J].中国粮油学报,2011,26(6):102-105.
[19]吴才章,徐振方,王继伟.小麦发光特性研究[J].中国粮油学报,2014,29(4):78-81.
[20]郑亚飞.两种蔬菜种子生物超弱发光与活力相关性的研究[J].德州学院学报,2010,26(6):5-7.
[21]张文兰,田茜,李群,等.低温保存对4种作物种子超弱发光特性影响规律的研究[J].山东农业科学,2014,46(12):38-42.
[22]国颖,杨洪强.高温对平邑甜茶幼苗生物发光与能量代谢的影响[J].园艺学报,2008,35(1):99-102.
[23]林桂玉,黄在范,张翠华,等.菊花花芽分化期超微弱发光及其生理代谢的变化[J].园艺学报,2008,35(12):1819-1824.
[24]张新华,杨洪强,李富军,等.杏花开放过程中超微弱发光和ATP及活性氧含量的变化[J].植物生理与分子生物学学报,2004,30(1):41-44.