玉米种子人工老化过程中超弱发光与生理代谢的关系（英文）
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摘要
以玉米品种"郑单958"种子为材料,研究了种子人工老化过程中超弱发光(ultraweak luminescence,UWL)的变化及其与种子活力、相对电导率、ATP含量的关系。结果表明,随着老化时间的延长,种子的发芽率、发芽势和发芽指数、UWL强度、ATP含量均逐渐下降,相对电导率逐渐升高;UWL强度与发芽率、发芽势、发芽指数和ATP含量显著正相关,与相对电导率显著负相关。表明,UWL与以ATP含量为标志的能量水平密切相关;UWL作为一种无损、灵敏、快速的物理检测指标,可应用于种子活力无损检测研究。
The seeds of maize(Zea mays L.) variety Zhengdan 958 were used to study the change of ultraweak luminescence( UWL) intensity and its relationship with seed vigor, relative electric conductivity and ATP content during artificial aging.The results indicated that the germination percentage, germination potential, germination index, UWL intensity and ATP content decreased gradually, while the relative electric conductivity increased with the prolongation of artificial aging. The correlation analysis showed that UWL intensity was significantly positively correlated with germination percentage, germination potential, germination index and ATP content, while significantly negatively correlated with relative electric conductivity. It was concluded that UWL was closely related to the energy level symbolized by ATP content, and as a fast nondestructive physical indicator with better sensitivity, it could be applied into the study of seed vigor nondestructive testing.
The seeds of maize(Zea mays L.) variety Zhengdan 958 were used to study the change of ultraweak luminescence( UWL) intensity and its relationship with seed vigor, relative electric conductivity and ATP content during artificial aging.The results indicated that the germination percentage, germination potential, germination index, UWL intensity and ATP content decreased gradually, while the relative electric conductivity increased with the prolongation of artificial aging. The correlation analysis showed that UWL intensity was significantly positively correlated with germination percentage, germination potential, germination index and ATP content, while significantly negatively correlated with relative electric conductivity. It was concluded that UWL was closely related to the energy level symbolized by ATP content, and as a fast nondestructive physical indicator with better sensitivity, it could be applied into the study of seed vigor nondestructive testing.
引文
[1]ZHANG XH(张新华),YANG HQ(杨洪强).Ultraweak bioluminescence in plan(植物的超微弱发光)[J].Journal o Shandong Agricultural University(Natural Science Edition)(山东农业大学学报(自然科学版)),2003,34(4):605-608.
[2]YANG HL(杨海莲),WANG HM(王红梅),YU JF(于家峰),et al.The influence of temperature treating on ultraweak luminescence from leaves(温度胁迫对叶片超弱发光的影响)[J].Journal of Sichuan Normal University(Natural Science)(四川师范大学学报(自然科学版)),2008,31(4):468-470.
[3]ZHU GY(朱冠宇),LIU H(刘欢),LIANGS(梁爽),et al.Changes of ultra-weak luminescence during the process of strawberry fruit development and maturation(草莓果实发育成熟过程中超微弱发光的变化)[J].Inner Mongolia Agricultural Science and Technology(内蒙古农业科技),2015,43(3):9-11.
[4]ZHANG XH(张新华),LI FJ(李富军),SHEN L(申琳),et al.Application of ultraweak luminescence technique in research of plant stress physiology(超弱发光技术在植物逆境生理研究中的应用)[J].Plant Physiology Communications(植物生理学通讯),2009,4(9):931-935.
[5]YAN Y,POPP F A,ROTHE G M.Correlation between germination capacity and biophoton emission of barley seeds(Hordeum vulgare L.)[J].Seed Sci.Technol.,2003,31(2):249-258.
[6]COSTANZO E,GULINO M,LANZANOL,et al.Single seed viability checked by delayed luminescence[J].Euro.Biophys.J.,2008,37(2):235-238.
[7]ZHANG JP(张菊平),ZHANG XZ(张兴志),GONG ZH(巩振辉).Application of ultraweak bioluminescence in vegetable research(超微弱发光在蔬菜研究中的应用)[J].Chinese Agricultural Science Bulletin(中国农学通报),2006,22(1):220-222.
[8]YUAN ZQ(袁佐清).Research progress of ultraweak bioluminescence(生物超微弱发光研究进展)[J].Journal of Anhui Agricultural Sciences(安徽农业科学),2008,36(8):3092-3094.
[9]CHEN W L,XING D,HE Y H.Determination the vigor of rice seed with different degrees of aging with ultraweak chemiluminescence during early imbition[J].Acta Bot.Sin.,2002,44(11):1376-1379.
[10]CHEN W L,XING D,WANG J,et al.Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition[J].Luminescence,2003,18(1):19-24.
[11]BAOJ(鲍杰),WU CZ(吴才章).Detection technique of wheat freshness based on virtual instrument(基于虚拟仪器的小麦活力检测)[J].journal Of The Chinese Cereals and Oils Association(中国粮油学报),2011,26(6):102-105.
[12]WU CZ(吴才章),XU ZF(徐振方),WANG JW(王继伟).Research on the Characteristics of Ultra-Weak Biophoton Emission from the Storage Wheat(小麦发光特性研究)[J].Journal of the Chinese Cereals and Oils Association(中国粮油学报),2014,29(4):78-81.
[13]ZHANG WL(张文兰),TIAN Q(田茜),LI Q(李群),et al.Effects of low-temperature preservation on ultra-weak luminescence of four species of crop seeds(低温保存对4种作物种子超弱发光特性影响规律的研究)[J].Shandong Agricultural Sciences(山东农业科学),2014,46(12):38-42.
[14]WANG CF(王春芳),ZHAN Y(詹耀),HU FF(胡菲菲),et al.Detection of the germination rate of rice and wheat basing on ultra weak luminescence technology(基于超弱发光技术的稻谷和小麦种子发芽率检测)[J].Journal of the Chinese Cereals and Oils Association(中国粮油学报),2003,28(2):105-199.
[15]Administration of Quality and Technology Supervision(国家技术监督局).Rules for agricultural seed testing(GB/T3543.3农作物种子检验规程)[S].Beijing:Standards Press of China(北京:中国标准出版社),1995.
[16]WANG WG(王维光),GU JB(顾俭本).Comparisons in leaf ATP extraction methods(从叶片中提取ATP方法的比较)[J].Plant Physiology Communications(植物生理学通讯),1986(5):54-55.
[17]WANG WG(王维光),HAN JY(韩俊英).Advance in the study of ultr-weak bioluminescent mechanisms and detecting methods(生物超弱发光机制及其检测方法研究进展)[J].Journal of Guangdong University of Technology(广东工业大学学报),17(1):49-54.
[18]ABLES F B.Plant chemiluminescence[J].Anun.Rev.Plant Physiol.1986,37:49-72.
[19]WANG HF(王华芳),YIN WL(尹伟伦),ZHENG CX(郑彩霞),et al.Ultraweak luminescence of plants(植物的超弱发光)[J].Journal of Beijing Forestry University(北京林业大学学报),1996,18(2):83-89.
[20]ZHANG XH(张新华),YANG HQ(杨洪强),LI FJ(李富军),et al.Changes in ultraweak luminescence,atp and active oxygen contents during apricot florescence(杏花开放过程中超微弱发光和ATP及活性氧含量的变化)[J].Journal of Plant Physiology and Molecular Biology(植物生理与分子生物学学报),2004,30(1):41-44.
[21]LIN GY(林桂玉),HUANG ZF(黄在范),ZHANG CH(张翠华),et al.Changes of ultraweak luminescence intensity,respiration rate and physiological metabolism of chrysanthemum during floral differentiation(菊花花芽分化期超微弱发光及其生理代谢的变化)[J].Acta Horticulturae Sinica(园艺学报),2008,35(12):1819-1824.
[2]YANG HL(杨海莲),WANG HM(王红梅),YU JF(于家峰),et al.The influence of temperature treating on ultraweak luminescence from leaves(温度胁迫对叶片超弱发光的影响)[J].Journal of Sichuan Normal University(Natural Science)(四川师范大学学报(自然科学版)),2008,31(4):468-470.
[3]ZHU GY(朱冠宇),LIU H(刘欢),LIANGS(梁爽),et al.Changes of ultra-weak luminescence during the process of strawberry fruit development and maturation(草莓果实发育成熟过程中超微弱发光的变化)[J].Inner Mongolia Agricultural Science and Technology(内蒙古农业科技),2015,43(3):9-11.
[4]ZHANG XH(张新华),LI FJ(李富军),SHEN L(申琳),et al.Application of ultraweak luminescence technique in research of plant stress physiology(超弱发光技术在植物逆境生理研究中的应用)[J].Plant Physiology Communications(植物生理学通讯),2009,4(9):931-935.
[5]YAN Y,POPP F A,ROTHE G M.Correlation between germination capacity and biophoton emission of barley seeds(Hordeum vulgare L.)[J].Seed Sci.Technol.,2003,31(2):249-258.
[6]COSTANZO E,GULINO M,LANZANOL,et al.Single seed viability checked by delayed luminescence[J].Euro.Biophys.J.,2008,37(2):235-238.
[7]ZHANG JP(张菊平),ZHANG XZ(张兴志),GONG ZH(巩振辉).Application of ultraweak bioluminescence in vegetable research(超微弱发光在蔬菜研究中的应用)[J].Chinese Agricultural Science Bulletin(中国农学通报),2006,22(1):220-222.
[8]YUAN ZQ(袁佐清).Research progress of ultraweak bioluminescence(生物超微弱发光研究进展)[J].Journal of Anhui Agricultural Sciences(安徽农业科学),2008,36(8):3092-3094.
[9]CHEN W L,XING D,HE Y H.Determination the vigor of rice seed with different degrees of aging with ultraweak chemiluminescence during early imbition[J].Acta Bot.Sin.,2002,44(11):1376-1379.
[10]CHEN W L,XING D,WANG J,et al.Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition[J].Luminescence,2003,18(1):19-24.
[11]BAOJ(鲍杰),WU CZ(吴才章).Detection technique of wheat freshness based on virtual instrument(基于虚拟仪器的小麦活力检测)[J].journal Of The Chinese Cereals and Oils Association(中国粮油学报),2011,26(6):102-105.
[12]WU CZ(吴才章),XU ZF(徐振方),WANG JW(王继伟).Research on the Characteristics of Ultra-Weak Biophoton Emission from the Storage Wheat(小麦发光特性研究)[J].Journal of the Chinese Cereals and Oils Association(中国粮油学报),2014,29(4):78-81.
[13]ZHANG WL(张文兰),TIAN Q(田茜),LI Q(李群),et al.Effects of low-temperature preservation on ultra-weak luminescence of four species of crop seeds(低温保存对4种作物种子超弱发光特性影响规律的研究)[J].Shandong Agricultural Sciences(山东农业科学),2014,46(12):38-42.
[14]WANG CF(王春芳),ZHAN Y(詹耀),HU FF(胡菲菲),et al.Detection of the germination rate of rice and wheat basing on ultra weak luminescence technology(基于超弱发光技术的稻谷和小麦种子发芽率检测)[J].Journal of the Chinese Cereals and Oils Association(中国粮油学报),2003,28(2):105-199.
[15]Administration of Quality and Technology Supervision(国家技术监督局).Rules for agricultural seed testing(GB/T3543.3农作物种子检验规程)[S].Beijing:Standards Press of China(北京:中国标准出版社),1995.
[16]WANG WG(王维光),GU JB(顾俭本).Comparisons in leaf ATP extraction methods(从叶片中提取ATP方法的比较)[J].Plant Physiology Communications(植物生理学通讯),1986(5):54-55.
[17]WANG WG(王维光),HAN JY(韩俊英).Advance in the study of ultr-weak bioluminescent mechanisms and detecting methods(生物超弱发光机制及其检测方法研究进展)[J].Journal of Guangdong University of Technology(广东工业大学学报),17(1):49-54.
[18]ABLES F B.Plant chemiluminescence[J].Anun.Rev.Plant Physiol.1986,37:49-72.
[19]WANG HF(王华芳),YIN WL(尹伟伦),ZHENG CX(郑彩霞),et al.Ultraweak luminescence of plants(植物的超弱发光)[J].Journal of Beijing Forestry University(北京林业大学学报),1996,18(2):83-89.
[20]ZHANG XH(张新华),YANG HQ(杨洪强),LI FJ(李富军),et al.Changes in ultraweak luminescence,atp and active oxygen contents during apricot florescence(杏花开放过程中超微弱发光和ATP及活性氧含量的变化)[J].Journal of Plant Physiology and Molecular Biology(植物生理与分子生物学学报),2004,30(1):41-44.
[21]LIN GY(林桂玉),HUANG ZF(黄在范),ZHANG CH(张翠华),et al.Changes of ultraweak luminescence intensity,respiration rate and physiological metabolism of chrysanthemum during floral differentiation(菊花花芽分化期超微弱发光及其生理代谢的变化)[J].Acta Horticulturae Sinica(园艺学报),2008,35(12):1819-1824.