小麦超弱发光特性研究
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摘要
应用一种超高灵敏光电倍增管,设计一套用于测量粮食超弱发光的数字单光子计数在线检测系统,实现对储藏条件下粮食超弱发光的绝对量测量。对不同品种、不同新陈度小麦超弱发光进行系统测试。结果表明,在常温储藏条件下,小麦自身的超弱发光是一个稳定的辐射过程,其发光强度在几十个光子/(cm2·s)左右;同一品种,不同新陈度小麦样品超弱发光能力差异明显,随着老化程度的增加,脂肪酸值逐渐增大,其超弱发光强度逐渐减弱,小麦自身超弱发光与其脂肪酸值负相关。在此基础上测量小麦样品在不同温度条件下超弱发光的辐射强度,研究小麦超弱发光随自身温度变化的规律。
A high sensitive single photon counting system with a new photomultiplier tube has been adopted to detect the ultra- weak biophoton emission from wheat. It could fulfill the absolute quantity detection of photon emitted from wheat in storage condition. The ultra- weak biophoton emission from various breeds and freshness has been measured and analyzed. It is demonstrated that the ultra- weak biophoton emission from wheat is a steady radiation process under room temperature. The intensity of ultra- weak photon emission from wheat is about scores of photons /( cm2·s). The difference of the biophoton emission ability for different freshness with the same breed is significant has been also found. As the degree of aging increases,the fatty acids becomes bigger,while the ultra- weak biophoton emission becomes weaker,which verified the negative correlation between the biophoton emission and the fatty acids. Finally,the ultra- weak biophoton emission from wheat has been measured under different temperatures,and the changing regularities of the ultra- weak photon emission from wheat depended on its temperature have been studied.
A high sensitive single photon counting system with a new photomultiplier tube has been adopted to detect the ultra- weak biophoton emission from wheat. It could fulfill the absolute quantity detection of photon emitted from wheat in storage condition. The ultra- weak biophoton emission from various breeds and freshness has been measured and analyzed. It is demonstrated that the ultra- weak biophoton emission from wheat is a steady radiation process under room temperature. The intensity of ultra- weak photon emission from wheat is about scores of photons /( cm2·s). The difference of the biophoton emission ability for different freshness with the same breed is significant has been also found. As the degree of aging increases,the fatty acids becomes bigger,while the ultra- weak biophoton emission becomes weaker,which verified the negative correlation between the biophoton emission and the fatty acids. Finally,the ultra- weak biophoton emission from wheat has been measured under different temperatures,and the changing regularities of the ultra- weak photon emission from wheat depended on its temperature have been studied.
引文
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[13]鲍杰,吴才章.基于虚拟仪器的小麦新陈度检测[J].中国粮油学报,2011,26(6):102-105
[14]唐庆,郑万芳,柯文,等.小麦脂肪酸值的变化规律及其在新鲜度判定中的应用[J].粮油仓储科技通讯,2007,27(4):45-47
[15]陈兆庆.小麦脂肪酸值是判断小麦新鲜度的一项指标[J].中国高新技术企业,2010,17(19):39-40.
[2]Musumeci F,Applegate L A,Privitera G,et al.Spectral analysis of laser-induced ultraweak delayed luminescence in cultured normal and tumor human cells:temperature dependence[J].Journal of Photochemistry and Photobiology B:Biology,2005,79(2):93-99
[3]Nigglia H J,Scaletta C,Yu Yan,et al.Ultraweak photon emission in assessing bone growth factor efficiency using fibroblastic differentiation[J].Journal of Photochemistry and Photobiology B:Biology,2001,64(1):62-68
[4]Havaux M.Spontaneous and thermoinduced photon emission:new methods to detect and quantify oxidative stress in plants[J].Trends in Plant Science,2003,8(9):409-413
[5]Yu Yan,Popp F A,Sigrist S,et al.Further analysis of delayed luminescence of plants[J].Journal of Photochemistry and Photobiology B:Biology,2005,78(3):235-244
[6]Kageyama C,Katoa K,Iyozumi H,et al.Photon emissions from rice cells elicited by N-acetylchitooligosaccharide are generated through phospholipid signaling in close association with the production of reactive oxygen species[J].Plant Physiology and Biochemistry,2006,44(11):901-909
[7]Colli L.Further measurement on the bioluminescence of the seedling[J].Experiment,1955,11(12):479
[8]Chen W L,Xing D,Wang J,et al.Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition[J].Luminescence,2003,18(1):19-24
[9]Triglia A,Grasso F,Musumeci F,et al.Temperature Dependence of the Ultraweak Spontaneous Photon Emission from Soya Seeds[J].Nuovo Cimento D,2003,15(11):1360-1370
[10]李韶山,王艳,郭周义,等.萌发花生种子超弱发光的研究[J].光子学报,2000,29(11):966-969
[11]郑亚飞.两种蔬菜种子生物超弱发光与活力相关性的的研究[J].德州学院学学报,2010,26(6):5-7
[12]吴文福,夏艳辉,周德义,等.干燥后玉米种子生物超弱发光与活力相关性的研究[J].农业工程学报,2002,18(3):8-10
[13]鲍杰,吴才章.基于虚拟仪器的小麦新陈度检测[J].中国粮油学报,2011,26(6):102-105
[14]唐庆,郑万芳,柯文,等.小麦脂肪酸值的变化规律及其在新鲜度判定中的应用[J].粮油仓储科技通讯,2007,27(4):45-47
[15]陈兆庆.小麦脂肪酸值是判断小麦新鲜度的一项指标[J].中国高新技术企业,2010,17(19):39-40.