海带多糖对超重环境下微波辐射损伤的防治
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摘要
目的探讨海带多糖对超重环境下微波辐射损伤的防治作用。方法制作大鼠超重环境下微波损伤模型,通过灌胃给予20 mg/ml海带多糖(低剂量组)和40 mg/ml海带多糖(高剂量组),观察防护效果。结果血浆中超氧阴离子在模型组显著低于对照组(P<0.05),低剂量海带多糖组和高剂量组与对照组比较差异无统计学意义(P>0.05)。丙二醛(MDA)和超氧化物歧化酶(SOD)在模型组与对照组差异无统计学意义(P>0.05),高剂量组及低剂量组显著高于对照组(P<0.05)。过氧化氢酶(CAT)在模型组显著低于对照组(P<0.05),而低剂量组与对照组比较差异无统计学意义(P>0.05)。谷胱甘肽过氧化物酶(GSH-POX)在模型组与对照组差异有统计学意义(P<0.05);高剂量组与对照组比较差异无统计学意义(P<0.05)。一氧化碳(NO)、一氧化碳合酶(NOS)和还原型合胱甘肽(GSH)在模型组、低剂量海带多糖组和高剂量组与对照组比较差异有统计学意义(P<0.05)。在肝组织匀浆中,过氧化氢酶(CAT)在模型组降低,但与对照组比较差异无统计学意义(P>0.05);在低剂量组与对照组比较差异无统计学意义(P>0.05)。NOS在模型组显著高于对照组(P<0.05),但低剂量组和高剂量组与对照组比较差异无统计学意义(P>0.05)。GSH-POX在模型组显著低于对照组(P<0.05)。GSH在模型组显著高于对照组(P<0.05),低剂量组与对照组比较差异无统计学意义(P>0.05)。结论海带对超重微波的过氧化损伤的防护的调节可能通过CAT及GSH-POX的清除和增强还原型GSH的抗氧化来实现,具体机制有待进一步研究。
【Objective】 To research prevention and treatment effects of laminarin on overoxidation damage of microwave radiation in rats under supergravity. 【Methods】 The rat model of microwave damage under supergravity was made. The rats were given 20 mg/ml(lower-dose group) or 40 mg/ml laminarin(high-dose group) by intragastric administration and the protective effects were observed. 【Results】 The plasma superoxide anion was significantly lower in the model group than in the control group(P < 0.05), but not significantly different between either the lower-dose or high-dose group and the control group(P > 0.05). MDA and SOD were not obviously different between the model group and control group(P > 0.05). MDA and SOD in the lower-dose and high-dose groups were significantly higher than those in the control group(P < 0.05). Catalase(CAT) decreased significantly in the model group compared with the control group(P < 0.05). GSH-POX had a significant difference between the model group and control group(P < 0.05), but no significant difference between the high-dose group and the control group(P > 0.05). NO, NOS and GSH in the model group, lowerdose group and high-dose group were significantly different from those in the control group(P < 0.05). In liver homogenate CAT decreased in the model group, but had no obvious difference from that in the control group(P > 0.05). GSH-POX was significantly lower in the model group than that in the control group(P < 0.05).GSH was significantly higher in the model group than in the control group(P < 0.05), but was not significantly different between the lower-dose group and the control group(P > 0.05). 【Conclusions】 Laminarin regulation to overoxidation damage of microwave radiation under supergravity could clear reactive oxygen species by CAT and GSH-POX and increase GSH antioxidation power. The concrete mechanism needs further study.
【Objective】 To research prevention and treatment effects of laminarin on overoxidation damage of microwave radiation in rats under supergravity. 【Methods】 The rat model of microwave damage under supergravity was made. The rats were given 20 mg/ml(lower-dose group) or 40 mg/ml laminarin(high-dose group) by intragastric administration and the protective effects were observed. 【Results】 The plasma superoxide anion was significantly lower in the model group than in the control group(P < 0.05), but not significantly different between either the lower-dose or high-dose group and the control group(P > 0.05). MDA and SOD were not obviously different between the model group and control group(P > 0.05). MDA and SOD in the lower-dose and high-dose groups were significantly higher than those in the control group(P < 0.05). Catalase(CAT) decreased significantly in the model group compared with the control group(P < 0.05). GSH-POX had a significant difference between the model group and control group(P < 0.05), but no significant difference between the high-dose group and the control group(P > 0.05). NO, NOS and GSH in the model group, lowerdose group and high-dose group were significantly different from those in the control group(P < 0.05). In liver homogenate CAT decreased in the model group, but had no obvious difference from that in the control group(P > 0.05). GSH-POX was significantly lower in the model group than that in the control group(P < 0.05).GSH was significantly higher in the model group than in the control group(P < 0.05), but was not significantly different between the lower-dose group and the control group(P > 0.05). 【Conclusions】 Laminarin regulation to overoxidation damage of microwave radiation under supergravity could clear reactive oxygen species by CAT and GSH-POX and increase GSH antioxidation power. The concrete mechanism needs further study.
引文
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[14]陈耀明,陈景元,刘秀红,等.补锌对微波辐照大鼠脑组织氧化损伤的影响[J].第四军医大学学报,2005,26(5):444-446.
[15]杨瑛杰,于蕾,季宇彬.2 450 MHz微波对受孕母鼠血清自由基的影响[J].中国伤残医学,2010,18(1):31-33.
[2]PANKRATOV TA.Acidobacteria in microbial communities of the bog and tundra lichens[J].Microbiology,2012,81(1):51-58.
[3]ZHANG C,GAO J,ZHANG L.Effect of laminarin on the expression of GRP78 and GRP94 in rat after unilateral ureteral obstruction[J].Scandinavian Journal of Urology and Nephrology,2012,46(4):267-272.
[4]罗琼,任世成,杨明亮,等.海带多糖对睾丸受辐射雄性大鼠性功能的影响[J].营养学报,2009,31(3):259-262.
[5]刘军,罗琼,杨明亮,等.海带多糖干预对慢性电离辐射损伤后雄性大鼠生殖功能的影响[J].食品科学,2010,31(7):293-296.
[6]罗琼,吴晓旻,杨明亮,等.海带多糖的抗辐射作用与淋巴细胞凋亡关系研究[J].营养学报,2004,2(6):471-473.
[7]刘军,罗琼,阎俊,等.海带多糖对慢性局部电离辐射致大鼠睾丸细胞损伤的干预效应研究[J].营养学报,2011,33(1):61-65.
[8]李菁菁,罗琼,周银柱,等.多糖联合作用对辐射大鼠睾丸组织损伤恢复影响[J].中国公共卫生,2011,27(11):1450-1453.
[9]王承志,吕士杰,芦晓静,等.正交试验法优选海带多糖的最佳提取工艺[J].安徽农业科学,2011,39(35):21778.
[10]JIANG YX,LV SJ,LU XJ,et al.Orthogonal test design for optimization of the technology of laminarin from laminaria japomica aresch[J].World Automation Cogress,2012:1-4.
[11]丛建波,张清俊,王长振,等.高功率脉冲微波辐射对小鼠机体氧化应激及巨噬细胞氧耗的影响[J].军事医学科学院院刊,2009,33(3):213-215.
[12]CHENG D,LIANG B,LI M.Influence of laminarin polysaccahrides on oxidative damage[J].International Journal of Biological Macromolecules,2011,48(1):63-66.
[13]NOH MY,JO YH,PARK CH,et al.Cloning and induction patterns of Cu/Zn superoxide dismutase in responses to immune elicitors and nucleopolyhedrovirus in the beet armyworm Spodoptera exigua[J].Entomological Research,2010,40(6):336-342.
[14]陈耀明,陈景元,刘秀红,等.补锌对微波辐照大鼠脑组织氧化损伤的影响[J].第四军医大学学报,2005,26(5):444-446.
[15]杨瑛杰,于蕾,季宇彬.2 450 MHz微波对受孕母鼠血清自由基的影响[J].中国伤残医学,2010,18(1):31-33.