对照提取物在银杏叶制剂特征图谱中的应用
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摘要
目的建立银杏叶片、胶囊、滴丸特征图谱的评价方法。方法以对照提取物为参照,采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法,色谱柱为Agilent Poroshell 120 EC-C18(150 mm×4.6 mm,2.7μm),流动相为甲醇-0.1%甲酸梯度洗脱,流速为0.8 mL·min~(-1);ELSD检测器:漂移管温度105℃;氮气(N2)流速:2.5 L·min~(-1)。结果以银杏叶对照提取物和银杏叶总内酯对照提取物为参照,确立12个共有峰作为银杏叶制剂的特征峰,建立了银杏叶制剂的特征图谱。结论该方法简单、重复性良好,可为银杏叶制剂的质量控制提供有效鉴别方法。
Objective To establish the characteristic spectrum of ginkgo leaf tablets,ginkgo leaf capsules,and ginkgo leaf dropping pills. Methods HPLC-ELSD analysis was performed on an Agilent Poroshell 120 EC-C_(18) column( 150 mm ×4.6 mm,2.7 μm) with the methanol-0.1% formic acid as mobile phase at the gradient elution mode,flow rate was 0.8 mL·min~(-1).Results Referring to the reference extract,a total of 12 peaks were established in the characteristic spectrum and selected as the characteristic common peaks. Conclusion The established method was simple and sensitive with good reproducibility,so it could be used to control the quality of ginkgo leaf preparations.
Objective To establish the characteristic spectrum of ginkgo leaf tablets,ginkgo leaf capsules,and ginkgo leaf dropping pills. Methods HPLC-ELSD analysis was performed on an Agilent Poroshell 120 EC-C_(18) column( 150 mm ×4.6 mm,2.7 μm) with the methanol-0.1% formic acid as mobile phase at the gradient elution mode,flow rate was 0.8 mL·min~(-1).Results Referring to the reference extract,a total of 12 peaks were established in the characteristic spectrum and selected as the characteristic common peaks. Conclusion The established method was simple and sensitive with good reproducibility,so it could be used to control the quality of ginkgo leaf preparations.
引文
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[2]蔡华,叶方,黄良永,等.超高效液相质谱联用法研究柴胡特征指纹图谱[J].中国医院药学杂志,2014,34(5):359-364.
[3]施菁,王炜,陈柳蓉.白花土元胡药材的高效液相色谱法指纹图谱研究[J].医药导报,2008,27(9):1108-1110.
[4]王桂红,张远远,肖伊,等.不同产地个青皮高效液相色谱指纹图谱研究[J].医药导报,2011,30(4):429-431.
[5]王京辉,杜小伟,王萌萌,等.舒血宁注射液、银杏叶提取物及银杏叶指纹图谱研究[J].药物分析杂志,2008,28(7):1026-1030.
[6]赵一懿,王京辉,傅欣彤,等.银杏叶药材黄酮类成分指纹图谱研究及其中11种黄酮苷类成分定量分析[J].中国药学杂志,2013,48(18):1583-1588.
[7]陆晓峰,彭国平,金灯萍.银杏叶内酯成分的指纹图谱研究[J].天然产物研究与开发,2005,17(4):460-462.
[8]周欣,李章万,张雪琴,等.银杏叶片剂中银杏黄酮的HPLC指纹图谱研究[J].中国药学杂志,2005,40(2):93-95.
[9]王敏娟,李静,李杨,等.玄参饮片HPLC特征图谱研究[J].药物分析杂志,2010,30(4):657-660.
[10]刘东辉,杨丽娥,黄月纯,等.铁皮枫斗HPLC特征图谱研究[J].中国现代应用药学,2013,30(4):390-394.
[11]陆静娴,祝明,陈勇,等.乌灵胶囊的HPLC化学成分特征图谱研究[J].药物分析杂志,2011,31(4):764-767.
[12]杨桃,陈新,李龙,等.鄂产竹节参高效液相特征图谱的建立和分析[J].医药导报,2013,32(11):1503-1507.