低温胁迫对紫花苜蓿越冬芽组织显微结构的影响
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摘要
为了探究青藏高原地区独特的气候条件下青大1号紫花苜蓿新品系低温胁迫下组织显微结构变化,试验通过显微结构观测,探索冷胁迫下青大1号紫花苜蓿新品系越冬芽组织结构变化情况。结果表明:随着胁迫时间的逐渐延长,新品系紫花苜蓿越冬芽组织结构越发紊乱,外表皮出现破裂现象,部分细胞壁结构出现明显变形;在-20℃处理24 h,部分紫花苜蓿越冬芽组织细胞核及细胞器即出现解离现象。
The aim of this experiment was to explore the effect of low temperature stress on tissue microstructural changes of the new line Qingda No.1 Alfalfa under the special climate conditions in Qinghai-Tibet Plateau area. The observation method of microstructure was employed in this experiment to research bud tissue structural changes of the new line Qingda No.1 Alfalfa under low temperature stress. The results indicated that the more cold stress time increased,the more disordered tissue microstructure of overwintering bud of the new line Alfalfa got. Outer skin happened to fracture phenomena and obvious distortion was found in the structure of partial cell walls. After a 24 h treatment at-20 ℃,dissociation phenomena appeared in the cell nucleus and other organelles of part of overwintering bud of Alfalfa.
The aim of this experiment was to explore the effect of low temperature stress on tissue microstructural changes of the new line Qingda No.1 Alfalfa under the special climate conditions in Qinghai-Tibet Plateau area. The observation method of microstructure was employed in this experiment to research bud tissue structural changes of the new line Qingda No.1 Alfalfa under low temperature stress. The results indicated that the more cold stress time increased,the more disordered tissue microstructure of overwintering bud of the new line Alfalfa got. Outer skin happened to fracture phenomena and obvious distortion was found in the structure of partial cell walls. After a 24 h treatment at-20 ℃,dissociation phenomena appeared in the cell nucleus and other organelles of part of overwintering bud of Alfalfa.
引文
[1]孙启忠,王育青,侯向阳.紫花苜蓿越冬性研究概述[J].草业科学,2004,21(3):21-25.
[2]赵曼容,贾恢先.植物学使用技术[M].兰州:甘肃民族出版社,1993.
[3]李正理.植物制片技术[M].北京:科学出版社,1987.
[4]VIANDS D R.Variability and selection for characters associated with root regeneration capability in alfalfa[J].Crop Sci,1998,28(1):232-236.
[5]STOUT P C,HALL J W.Fall growth and winter survival of alfalfa in interior British Columbia[J].Can J Plant Sci,1989,69(1):491-499.
[6]刘志鹏,杨青川,呼天明.侧根型紫花苜蓿遗传基础及其育种研究进展[J].中国草地,2003,25(3):66-71.
[7]梁慧敏,夏阳.苜蓿抗寒性及根蘖性状的表现与过氧化物酶同工酶关系的研究[J].草业学报,1998,7(4):55-60.
[8]SULC R M,ALBRECHT K A,DUKE S H.Leakage of intracellular substances as an indicator of freezing injury in alfalfa[J].Crop Sci,1991,31(2):430-435.
[9]PEDERSON G A,HILL R R JR,KENDALL W A.Genetic variability for root characters in alfalfa populations differing in winter hardiness[J].Crop Sci,1984,24(3):465-468.
[10]魏兴琥,杨萍,李森,等.超载放牧与那曲地区高山嵩草草甸植被退化及其退化指标的探讨[J].草业学报,2005,14(3):41-49.
[2]赵曼容,贾恢先.植物学使用技术[M].兰州:甘肃民族出版社,1993.
[3]李正理.植物制片技术[M].北京:科学出版社,1987.
[4]VIANDS D R.Variability and selection for characters associated with root regeneration capability in alfalfa[J].Crop Sci,1998,28(1):232-236.
[5]STOUT P C,HALL J W.Fall growth and winter survival of alfalfa in interior British Columbia[J].Can J Plant Sci,1989,69(1):491-499.
[6]刘志鹏,杨青川,呼天明.侧根型紫花苜蓿遗传基础及其育种研究进展[J].中国草地,2003,25(3):66-71.
[7]梁慧敏,夏阳.苜蓿抗寒性及根蘖性状的表现与过氧化物酶同工酶关系的研究[J].草业学报,1998,7(4):55-60.
[8]SULC R M,ALBRECHT K A,DUKE S H.Leakage of intracellular substances as an indicator of freezing injury in alfalfa[J].Crop Sci,1991,31(2):430-435.
[9]PEDERSON G A,HILL R R JR,KENDALL W A.Genetic variability for root characters in alfalfa populations differing in winter hardiness[J].Crop Sci,1984,24(3):465-468.
[10]魏兴琥,杨萍,李森,等.超载放牧与那曲地区高山嵩草草甸植被退化及其退化指标的探讨[J].草业学报,2005,14(3):41-49.