氮肥和植物激素对龙胆越冬芽形成的影响
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摘要
龙胆越冬芽的数量和发育状况决定翌年地上植株的生长发育,进而影响根系生长和产量,而越冬芽形成受植物内源激素调控。采用HPLC-MS/MS法测定了龙胆越冬芽发育各时期的内源激素含量,并阐明了各种内源激素对龙胆越冬芽形成的作用及作用时期。通过施用氮肥和外源激素,考察其对龙胆越冬芽形成的影响。结果表明:在龙胆越冬芽未形成和形成初期施以氮肥及外源激素,可以有效地促进越冬芽形成,增加越冬芽数量。
The number and development of overwintering buds in Gentiana scabra determine the growth and development of aboveground plants in the following year,and then affect the growth and yield of the roots,while the overwintering bud formation is regulated by endogenous hormones. In this study,HPLC-MS/MS method was used to determine the content of endogenous hormones during different development stages of overwintering buds,and the effects of various endogenous hormones on the formation of overwintering buds and their corresponding stages were analyzed and explained. The effects of nitrogen fertilizer and exogenous hormones on the number of overwintering buds were investigated. The results showed that nitrogen and exogenous hormones could effectively increase the number of overwintering buds during the stage before overwintering bud formation and the early stage of overwintering bud formation.
The number and development of overwintering buds in Gentiana scabra determine the growth and development of aboveground plants in the following year,and then affect the growth and yield of the roots,while the overwintering bud formation is regulated by endogenous hormones. In this study,HPLC-MS/MS method was used to determine the content of endogenous hormones during different development stages of overwintering buds,and the effects of various endogenous hormones on the formation of overwintering buds and their corresponding stages were analyzed and explained. The effects of nitrogen fertilizer and exogenous hormones on the number of overwintering buds were investigated. The results showed that nitrogen and exogenous hormones could effectively increase the number of overwintering buds during the stage before overwintering bud formation and the early stage of overwintering bud formation.
引文
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[21]李俊香,温超,刘凤栾,等.温度对切花菊‘深志’侧芽形成的影响[J].中国农业大学学报,2014,19(1):74-79.
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[2]沈涛,李远菊,张霁,等.林药复合栽培滇龙胆HPLC指纹图谱计量特征与质量评价[J].中国药学杂志,2015,50(7):579-585.
[3]Takahashi H,Uchimiya H.The gentio-oligosaccharide gentiobiose functions in the modulation of bud dormancy in the herbaceous Perennial Gentiana.[J].Plant Cell,2014,26(10):3949-3963.
[4]Sun Lili,Jiang Tian,Zhang Haiyan,et al.Phytohormone regulation of root growth triggered by P deficiency or Al toxicity[J].Journal of Experimental Botany,2016,67(12):3655-3664.
[5]Fu Jing,Huang Zuanhua,Wang Zhiqin,et al.Pre-anthesis non-structural carbohydrate reserve in the stem enhances the sink strength of inferior spikelets during grain filling of rice[J].Field Crops Research,2011,123:170-182.
[6]Hosokawa K,Nakano M,Oikawa Y,et al.Adventitious shoot regeneration from leaf,stem and root explants of commercial cultivars of Gentiana[J].Plant Cell Reports,1996,15(8):578-581.
[7]刘霞,唐铭,许永华,等.茖葱不定芽分化的再生体系[J].吉林农业大学学报,2015,37(4):434-439.
[8]毕晓颖,张晓冬,魏秀娟,等.非洲菊叶片愈伤组织诱导和不定芽分化的研究[J].吉林农业大学学报,2014,36(1):66-70.
[9]张荻.百子莲花芽分化及开花机理研究[D].哈尔滨:东北林业大学,2011.
[10]马有宁.液相串联质谱测定水稻37种内源激素方法的研究[D].北京:中国农业科学院,2011.
[11]许智宏,薛红卫.植物激素作用的分子机理[M].上海:上海科学技术出版社,2012:1-10.
[12]Suzuki T,Miwa K,Ishikawa K,et al.The Arabidopsis sensor His-kinase,AHk4,can respond to cytokinins.[J].Plant&Cell Physiology,2001,42(2):107-113.
[13]Imamura T,Higuchi A,Sekine K T.High concentrations of sucrose induce overwintering bud formation in gentian plantlets cultured in vitro[J].Plant Biotechnology,2014,31(2):97-104.
[14]Schoellhorn R K,Barrett J E,Bartuska C A,et al.Elevated temperature affects axillary meristem development in dendranthema×grandiflorum‘Improved Mefo’[J].Hortscience A Publication of the American Society for Horticultural Science,2001,36(6):1049-1052.
[15]Huh Y J,Jin H L,Sang K P,et al.Endogenous indole-3-acetic acid and trans-zeatin ribosides in relation to axillary bud formation in standard chrysanthemum[J].Horticulture,Environment,and Biotechnology,2011,52(2):128-132.
[16]Vishwakarma K,Upadhyay N,Kumar N,et al.Abscisic acid signaling and abiotic stress tolerance in plants:A review on current knowledge and future prospects[J].Frontiers in Plant Science,2017,8:161.
[17]Achard P,Gusti A C S,Alioua M,et al.Gibberellin signaling controls cell proliferation rate in Arabidopsis[J].Current Biology,2009,19(14):1188.
[18]Susana Ubeda-Toms,Femn Federici,Uda Casimiro,et al.Gibberelin signaling in the endodermis controls Arabidopsis root meristem size[J].Current Biology,2009,19(14):1194-1199.
[19]Takahashi H,Nishihara M.Gentiobiose feeding in gentian in vitro overwintering buds or plantlets[J].Bio-Protocal,2015,5(12):1499.
[20]刘杨,丁艳锋,王强盛,等.植物生长调节剂对水稻分蘖芽生长和内源激素变化的调控效应[J].作物学报,2011,37(4):670-676.
[21]李俊香,温超,刘凤栾,等.温度对切花菊‘深志’侧芽形成的影响[J].中国农业大学学报,2014,19(1):74-79.
[22]姜贝贝.菊花侧芽形成过程中内源激素变化及Dg Ls L基因遗传转化研究[D].南京:南京农业大学,2009.