当归红芪超滤物对顺铂诱导大鼠肾足细胞损伤的保护作用研究
|
摘要
目的探究当归红芪超滤物(RAS-RH)对顺铂体外诱导大鼠肾足细胞损伤的保护作用。方法本实验选取大鼠肾足细胞分为对照组、药物组、顺铂组及联合组,采用倒置相差显微镜观察顺铂对大鼠肾足细胞生长的影响,利用实时细胞分析技术监测给予RAS-RH前、后细胞的生长指数(CI),流式细胞仪检测大鼠肾足细胞的凋亡率。结果顺铂对大鼠肾足细胞生长增殖具有明显抑制作用,联合组对顺铂诱导的大鼠肾足细胞损伤具显著的保护作用;流式细胞仪显示,联合组大鼠肾足细胞凋亡率明显低于顺铂组凋亡率(P<0.05)。结论 RAS-RH对顺铂诱导的体外大鼠肾足细胞损伤具有明显的保护作用。
Objective To investigate the protective effects of Radix Angelicae Sinensis and Radix Hedysari(RAS-RH)on rat kidney podocytes injury induced by cisplatin in vitro.Methods Rat kidney podocytes were divided into the control group,the drug group,the cisplatin group and the combination group.Cisplatin influence on rat kidney podocytes was observed by inverted phase contrast microscope.Cell growth index(CI)before and after RAS-RH was observed by real-time cell analysis,and the apoptosis rate was measured by flow cytometry.Results Cisplatin significantly inhibited the growth and proliferation of rat kidney podocytes.And the combination group showed significant protective effects on cisplatin-induced kidney podocytes injury.Flow cytometry showed that the apoptosis rate of kidney podocytes in the combination group was significantly lower than that in the cisplatin group(P<0.05).Conclusion RAS-RH had obvious protective effects on cisplatin-induced kidney podocytes injury in vitro.
Objective To investigate the protective effects of Radix Angelicae Sinensis and Radix Hedysari(RAS-RH)on rat kidney podocytes injury induced by cisplatin in vitro.Methods Rat kidney podocytes were divided into the control group,the drug group,the cisplatin group and the combination group.Cisplatin influence on rat kidney podocytes was observed by inverted phase contrast microscope.Cell growth index(CI)before and after RAS-RH was observed by real-time cell analysis,and the apoptosis rate was measured by flow cytometry.Results Cisplatin significantly inhibited the growth and proliferation of rat kidney podocytes.And the combination group showed significant protective effects on cisplatin-induced kidney podocytes injury.Flow cytometry showed that the apoptosis rate of kidney podocytes in the combination group was significantly lower than that in the cisplatin group(P<0.05).Conclusion RAS-RH had obvious protective effects on cisplatin-induced kidney podocytes injury in vitro.
引文
[1]张静,周文.顺铂肾毒性相关机制及其防护研究现状[J].中国药学杂志,2012,47(22):1785-1789.
[2]樊秦,李应东,舒畅,等.超滤膜分离纯化当归补血汤的研究[J].中成药,2010,32(8):1438-1440.
[3]国家药典委员会.中华人民共和国药典:2010年版[M].北京:中国医药科技出版社,2010:124-142.
[4]王英,张尚智,朱田田,等.当归有效成分的提取与分析研究进展[J].中兽医医药杂志,2016,35(2):75-79.
[5]柳小亚,李继平,陈心悦,等.HPLC同时测定红芪中8个活性成分的含量及聚类分析[J].药学学报,2016,51(5):786-791.
[6]寇宁,李磊强,李钦,等.不同提取方法对红芪多糖体外抗氧化活性的影响研究[J].食品工业科技,2015,36(15):100-103.
[7]朱贝贝,刘斌,李淑玲,等.当归红芪超滤膜提取物对过氧化氢致PC12细胞氧化损伤的影响[J].世界中西医结合杂志,2016,11(9):1228-1232.
[8]赵昱波,陈俊,许浚,等.红芪的化学成分及抗肿瘤作用研究进展[J].中草药,2015,46(22):3434-3440.
[9]王小军,刘华.红芪多糖-1对人肺腺癌A549细胞氧化应激的影响[J].中国呼吸与危重监护杂志,2017,16(2):127-131.
[10]刘华,闫立萍,王小军.红芪多糖诱导肺腺癌细胞凋亡与Bax/Bcl-2表达的研究[J].西北国防医学杂志,2016,37(4):211-213.
[11]张艳霞,张延英,张正顺,等.当归挥发油对人肺腺癌SPC-A-1细胞增殖及凋亡的影响[J].中药药理与临床,2016,32(6):102-105.
[12]安静,赵博琛,吴清,等.当归提取物配伍应用对小鼠结直肠癌的预防作用[J].北京中医药大学学报,2014,37(5):309-313.
[13]钱超,陆清昀,张晓春.癌症患者顺铂化疗相关肾毒性的临床进展[J].实用癌症杂志,2014,29(5):607-609.
[14]ASANUMA K,MUNDEL P.The role of podocytes in glomerular pathobiology[J].Clin Exp Nephrol,2013,7(4):255-259.
[15]郦豪,陶伟,姚日生.抗肿瘤药物顺铂及其纳米载体的研究进展[J].安徽化工,2013,39(5):5-9.
[2]樊秦,李应东,舒畅,等.超滤膜分离纯化当归补血汤的研究[J].中成药,2010,32(8):1438-1440.
[3]国家药典委员会.中华人民共和国药典:2010年版[M].北京:中国医药科技出版社,2010:124-142.
[4]王英,张尚智,朱田田,等.当归有效成分的提取与分析研究进展[J].中兽医医药杂志,2016,35(2):75-79.
[5]柳小亚,李继平,陈心悦,等.HPLC同时测定红芪中8个活性成分的含量及聚类分析[J].药学学报,2016,51(5):786-791.
[6]寇宁,李磊强,李钦,等.不同提取方法对红芪多糖体外抗氧化活性的影响研究[J].食品工业科技,2015,36(15):100-103.
[7]朱贝贝,刘斌,李淑玲,等.当归红芪超滤膜提取物对过氧化氢致PC12细胞氧化损伤的影响[J].世界中西医结合杂志,2016,11(9):1228-1232.
[8]赵昱波,陈俊,许浚,等.红芪的化学成分及抗肿瘤作用研究进展[J].中草药,2015,46(22):3434-3440.
[9]王小军,刘华.红芪多糖-1对人肺腺癌A549细胞氧化应激的影响[J].中国呼吸与危重监护杂志,2017,16(2):127-131.
[10]刘华,闫立萍,王小军.红芪多糖诱导肺腺癌细胞凋亡与Bax/Bcl-2表达的研究[J].西北国防医学杂志,2016,37(4):211-213.
[11]张艳霞,张延英,张正顺,等.当归挥发油对人肺腺癌SPC-A-1细胞增殖及凋亡的影响[J].中药药理与临床,2016,32(6):102-105.
[12]安静,赵博琛,吴清,等.当归提取物配伍应用对小鼠结直肠癌的预防作用[J].北京中医药大学学报,2014,37(5):309-313.
[13]钱超,陆清昀,张晓春.癌症患者顺铂化疗相关肾毒性的临床进展[J].实用癌症杂志,2014,29(5):607-609.
[14]ASANUMA K,MUNDEL P.The role of podocytes in glomerular pathobiology[J].Clin Exp Nephrol,2013,7(4):255-259.
[15]郦豪,陶伟,姚日生.抗肿瘤药物顺铂及其纳米载体的研究进展[J].安徽化工,2013,39(5):5-9.