地榆七柏汤灌肠治疗溃疡性结肠炎的实验研究
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摘要
目的观察地榆七柏汤灌肠对溃疡性结肠炎(UC)大鼠的治疗效果。方法选80只清洁级Wistar雄性大鼠用于实验研究,空白组仅予盐水1 m L/100 g(鼠重)灌肠,其他组通过TNBS混合剂制备UC模型,之后模型组不给予药物治疗,柳氮磺胺吡啶(SASP)组和地榆七柏汤低(0. 5 g/m L)、中(1 g/m L)、高(3 g/m L)剂量组分别给予相应药物治疗。观察评估各组大鼠疾病活动指数(DAI)、血清IL-8和TNF-α水平以及结肠组织学变化。结果造模后,SASP组和不同剂量地榆七柏汤组大鼠DAI评分随时间延长而不断降低(P <0. 05),但SASP组和不同剂量地榆七柏汤组之间无统计学意义(P> 0. 05);造模后给予SASP或不同剂量地榆七柏汤灌肠均能明显降低TNF-α和IL-8水平(P <0. 05),但SASP和不同剂量地榆七柏汤组之间无统计学意义(P>0. 05);病理学检查显示,地榆七柏汤低剂量组能较好的修复结肠组织创面。结论地榆七柏汤能改善溃疡结肠炎大鼠的疾病活动性、降低血清TNF-α、IL-8水平,促进结肠组织创面修复。
Objective To observe the therapeutic effect of Diyuqibai decoction enema on ulcerative colitis( UC) rats. Methods Eighty clean Wistar male rats were selected for experimental study. The blank group was given saline enema of 1 m L/100 g( rat weight) only, the other groups were prepared UC model through TNBS mixture. After that, the model group was not given drug treatment. Sulfasalazine( SASP) group and Diyuqibai decoction low( 0. 5 g/m L), medium( 1 g/m L) and high( 3 g/m L) dose groups were given corresponding drug treatment. The disease activity index( DAI), serum levels of IL-8 and TNF-α, HE staining of colon tissue were observed and evaluated. Results After modeling, DAI scores of rats in SASP group and different doses of Diyuqibai decoction group decreased when time increase( P < 0. 05), but there was no significant difference between them( P > 0. 05). After modeling, SASP or different doses of Diyuqibai decoction could significantly reduce the levels of TNF-αand IL-8( P < 0. 05), but there was no significant difference between them( P > 0. 05). Pathological examination showed that the low dose Diyuqibai decoction group could repair the wound of the colon wound better. Conclusion Diyuqibai decoction can improve the DAI score of ulcerative colitis rats, reduce serum levels of TNF-αand IL-8, and promote the repair of colonic wounds.
Objective To observe the therapeutic effect of Diyuqibai decoction enema on ulcerative colitis( UC) rats. Methods Eighty clean Wistar male rats were selected for experimental study. The blank group was given saline enema of 1 m L/100 g( rat weight) only, the other groups were prepared UC model through TNBS mixture. After that, the model group was not given drug treatment. Sulfasalazine( SASP) group and Diyuqibai decoction low( 0. 5 g/m L), medium( 1 g/m L) and high( 3 g/m L) dose groups were given corresponding drug treatment. The disease activity index( DAI), serum levels of IL-8 and TNF-α, HE staining of colon tissue were observed and evaluated. Results After modeling, DAI scores of rats in SASP group and different doses of Diyuqibai decoction group decreased when time increase( P < 0. 05), but there was no significant difference between them( P > 0. 05). After modeling, SASP or different doses of Diyuqibai decoction could significantly reduce the levels of TNF-αand IL-8( P < 0. 05), but there was no significant difference between them( P > 0. 05). Pathological examination showed that the low dose Diyuqibai decoction group could repair the wound of the colon wound better. Conclusion Diyuqibai decoction can improve the DAI score of ulcerative colitis rats, reduce serum levels of TNF-αand IL-8, and promote the repair of colonic wounds.
引文
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[12]黄晓东,李一兵,郭文涛,等.肠愈汤口服联合康复新液灌肠治疗溃疡性结肠炎的临床效果观察[J].中国医药,2016,11(10):1510-1513.
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[15]杜斌,沈洪,王军,等.基于数据挖掘方法对溃疡性结肠炎灌肠方用药及配伍规律分析[J].辽宁中医杂志,2014(4):627-629.
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[18]王影,刘文娟,崔瑛.黄连现代研究进展[J].中医学报,2014,29(11):1642-1645.
[19]杨磊,张延英,李卉,等.黄柏煎剂的抗炎、抗菌作用研究[J].实验动物科学,2014,31(4):14-17.
[20]叶招浇,阎澜,李洪娇,等.中药地榆的药理作用及临床应用研究进展[J].药学服务与研究,2015,15(1):47-50.
[21]郭昕.胃溃疡白芨三七调藕粉[J].中华养生保健,2017(3):38.
[22]陈开平,马丽.中药汤剂内服及外用灌肠联合治疗溃疡性结肠炎的临床研究[J].四川中医,2017,35(3):78-80.
[2]孙钦娟,李琴,宛东,等.细胞因子在炎症性肠病患者肠黏膜中表达的分析[J].胃肠病学,2018,23(1):13-17.
[3]林安娜,李雨晴,钟慕晓,等.炎性细胞因子在溃疡性结肠炎患者中的表达及其对预后的影响[J].南方医科大学学报,2016,36(12):1712-1717.
[4]李薇,赵慧燕,胡凤林,等.李吉彦从痈论治溃疡性结肠炎经验介绍[J].新中医,2017,49(7):142-144.
[5]吴小庚,王爱磊,张学明,等.实验性溃疡性结肠炎大鼠模型的建立[J].河北医药,2011,33(4):493-495.
[6]Okayasu I,Hatakeyama S,Yamada M,et al.A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice[J].Gastroenterology,1990,98(3):694-702.
[7]陈璐.溃疡性结肠炎发病机制的研究进展[J].疑难病杂志,2016,15(6):650-654.
[8]詹立红.溃疡性结肠炎患者血清TNF-α、IL-8水平检测及临床意义[J].北方药学,2014,11(6):85-85.
[9]王艳萍,姬林松,倪猛,等.不同严重程度溃疡性结肠炎患者血清TNF-α、IL-6及IL-8的表达及意义[J].中国老年学,2015,35(14):3940-3941.
[10]冯振仁.中西医结合治疗溃疡性结肠炎60例临床观察[J].中国医药科学,2015,5(14):32-34.
[11]龙娟.美沙拉嗪联合双歧三联活菌对溃疡性结肠炎临床症状评分、DAI评分的影响[J].中国医学工程,2015,23(12):131-132.
[12]黄晓东,李一兵,郭文涛,等.肠愈汤口服联合康复新液灌肠治疗溃疡性结肠炎的临床效果观察[J].中国医药,2016,11(10):1510-1513.
[13]王童,葛亚萍,石立鹏,等.中医药治疗溃疡性结肠炎的研究进展[J].中医临床研究,2018,10(10):144-147.
[14]赵正平,滑永志,陆敏.溃疡性结肠炎灌肠疗法研究近况[J].光明中医,2014,29(12):2688-2690.
[15]杜斌,沈洪,王军,等.基于数据挖掘方法对溃疡性结肠炎灌肠方用药及配伍规律分析[J].辽宁中医杂志,2014(4):627-629.
[16]李斌,李鑫,范源.诃子药理作用研究进展[J].药学研究,2015,34(10):591-595,603.
[17]董鹏,薛洪利.诃子抗大鼠溃疡性结肠炎免疫机制实验研究[J].辽宁中医药大学学报,2014,16(6):41-44.
[18]王影,刘文娟,崔瑛.黄连现代研究进展[J].中医学报,2014,29(11):1642-1645.
[19]杨磊,张延英,李卉,等.黄柏煎剂的抗炎、抗菌作用研究[J].实验动物科学,2014,31(4):14-17.
[20]叶招浇,阎澜,李洪娇,等.中药地榆的药理作用及临床应用研究进展[J].药学服务与研究,2015,15(1):47-50.
[21]郭昕.胃溃疡白芨三七调藕粉[J].中华养生保健,2017(3):38.
[22]陈开平,马丽.中药汤剂内服及外用灌肠联合治疗溃疡性结肠炎的临床研究[J].四川中医,2017,35(3):78-80.