参麦注射液抗氧化活性体外实验研究
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摘要
[目的]系统研究参麦注射液体外抗氧化性能。[方法]采用铁还原抗氧化能力(FRAP)法、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法、一氧化氮(NO)自由基清除法、过氧化氢(H_2O_2)清除法、亚铁(Fe~(2+))离子螯合法分别测试不同稀释倍率参麦注射液的总抗氧化能力、DPPH·清除活性、NO·清除活性、H_2O_2清除活性和Fe~(2+)离子螯合活性,并将结果与0.1mg/mL 2,6-二叔丁基对甲酚(BHT)、10 mmol/L水溶性维生素E(Trolox)和10%柠檬酸的结果进行比较。[结果]参麦注射液具有一定的体外抗氧化能力,总抗氧化能力和对自由基清除活性均呈浓度正依赖性;10倍稀释参麦注射液总抗氧化能力显著低于BHT和Trolox(P<0.01),但高于柠檬酸(P<0.01);参麦注射液对不同自由基清除能力存在差异,其强弱顺序为:NO·[(54.4±6.6)%]>Fe~(2+)离子螯[(42.6±3.2)%]>DPPH·[(36.2±2.8)%]> H_2O_2[(23.5±1.2)%]。[结论]参麦注射液体外抗氧化活性研究将为其抗氧化药理学机制提供了实验依据。
[Objective] To systematic explore the antioxidant activities of Shenmai Injection. [Methods] The total antioxidant capability,DPPH·scavenging activity,NO·scavenging activity,H_2O_2 scavenging activity and Fe~(2+) chelating activity of the Shenmai Injection,which was diluted by different rates,were determined by Ferrric reducing antioxidant power assay(FRAR),DPPH· scavenging assay,NO·scavenging assay,H_2O_2 scavenging assay and Fe~(2+) chelating assay respectively. The results were compared with those of 0.1 mg/mL BHT,10 mmol/L Trolox and 10% citric acid. [Results] Shenmai Injection has certain antioxidant capacity in vitro. The total antioxidant capacity of Shenmai Injection was lower than that of BHT and Trolox(P <0.01),but higher than that of citric acid. The free radical scavenging activities of Shenmai Injection were positive concentration-dependence and were different for different free radical types. The order is NO· [(54.4±6.6)%] > Fe~(2+) ion [(42.6±3.2)%] > DPPH· [(36.2±2.8)%]> H_2O_2[(23.5±1.2)%]. [Conclusion] The in vitro experimental data of the antioxidant activities of Shenmai Injection will provide data support for its pharmacological mechanisms.
[Objective] To systematic explore the antioxidant activities of Shenmai Injection. [Methods] The total antioxidant capability,DPPH·scavenging activity,NO·scavenging activity,H_2O_2 scavenging activity and Fe~(2+) chelating activity of the Shenmai Injection,which was diluted by different rates,were determined by Ferrric reducing antioxidant power assay(FRAR),DPPH· scavenging assay,NO·scavenging assay,H_2O_2 scavenging assay and Fe~(2+) chelating assay respectively. The results were compared with those of 0.1 mg/mL BHT,10 mmol/L Trolox and 10% citric acid. [Results] Shenmai Injection has certain antioxidant capacity in vitro. The total antioxidant capacity of Shenmai Injection was lower than that of BHT and Trolox(P <0.01),but higher than that of citric acid. The free radical scavenging activities of Shenmai Injection were positive concentration-dependence and were different for different free radical types. The order is NO· [(54.4±6.6)%] > Fe~(2+) ion [(42.6±3.2)%] > DPPH· [(36.2±2.8)%]> H_2O_2[(23.5±1.2)%]. [Conclusion] The in vitro experimental data of the antioxidant activities of Shenmai Injection will provide data support for its pharmacological mechanisms.
引文
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[12]赵保路.利用电子自旋共振(ESR)技术研究一氧化氮自由基在心脑血管疾病和健康中的“双刃剑”作用(英文)[J].波谱学杂志,2015,32(2):195-207.
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[14] Lucius R,Sievers J. Postnatal retinal ganglion cells in vitro:protection against reactive oxygen species(ROS)-induced axonal degeneration by cocultured astrocytes[J].Brain Res.,1996,743(1-2):56-62.
[2]丁方莉,邵素娟,张艳东,王振宇.天然产物抗应激活性成分研究进展[J].食品工业科技,2018,39(1):333-336,341.
[3]黄泽清,胡铁宏.参麦注射液的药理和临床研究进展[J].临床医药文献电子杂志,2017,4(14):2762-2763.
[4]叶方斌,张荣.参麦注射液治疗缺血性脑卒中研究进展[J].中国实用神经疾病杂志,2011,14(12):94-96.
[5]李建生,张卫红,赵君玫,等.川芎嗪和参麦注射液对脑缺血-再灌注肺损伤老龄大鼠ATP酶和自由基代谢的影响[J].中国中西医结合急救杂志,2001,8(4):210-212.
[6]代向东,胡晓慧,闫海峰,等.丹红、参麦和参附3种注射液抗氧化能力的研究[J].天津中医药,2016,33(2):104-106.
[7] Dolai N,Karmakar I,Kumar RBS,et al. Free radical scavenging activity of Castanopsis indica in mediating hepatoprotective activity of carbon tetrachloride intoxicated rats. Asian Pac[J]. J Trop Biomed,2012:2,S243-S251.
[8] Rosen GM,Rauckman EJ. Spin trapping of superoxide and hydroxyl radicals[J]. Methods Enzymol,1984:105,198-209.
[9] Dinis TCP,Madeira VMC,Almeida LM. Action of phenolic derivatives(acetaminophen,salicylate,and 5-aminosalicylate)as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers[J]. Arch Biochem Biophys,1994:161-169,315.
[10] Bishayee A,Sethi G. Bioactive natural products in cancer prevention and therapy:Progress and promise[J]. Semin Cancer Biol.,2016,40-41:1-3.
[11]余健,辛艳飞,宣尧仙.参麦注射液药理作用的物质基础研究进展[J].医药导报,2013,32(4):497-500.
[12]赵保路.利用电子自旋共振(ESR)技术研究一氧化氮自由基在心脑血管疾病和健康中的“双刃剑”作用(英文)[J].波谱学杂志,2015,32(2):195-207.
[13] Wu JZ,Cheng CC,Shen LL,et al. Synthetic chalcones with potent antioxidant ability on H2O2-induced apoptosis in PC12 cells[J]. Int J Mol Sci,2014,15(10):18525-18539.
[14] Lucius R,Sievers J. Postnatal retinal ganglion cells in vitro:protection against reactive oxygen species(ROS)-induced axonal degeneration by cocultured astrocytes[J].Brain Res.,1996,743(1-2):56-62.