自组装2D-SERS基底膜用于塑化剂的检测研究
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摘要
采用液液界面自组装法在正己烷/水两相界面处制备出大面积且致密的二维(2D)表面增强拉曼光谱(SERS)基底膜。以正己烷为油相,配体置换后的金纳米棒(Au NRs)为水相,加入乙醇迅速诱导Au NRs在油-水界面处自组装出2D-SERS基底膜。采用聚乙烯吡咯烷酮(PVP)置换Au NRs表面的十六烷基三甲基溴化铵(CTAB),不仅有效地降低了Au NRs的表面电荷,而且减少了各纳米粒子之间的排斥力。随后以结晶紫(CV)为拉曼探针分子,考察了该基底膜具有较高的灵敏度和较好的重复性,并检测了塑化剂BBP。结果表明,该基底膜对BBP的检测限达到10~(-8) mol/L,并且能成功检测出某市售白酒中添加的中毒剂量(1.3 mg/kg)的BBP,因此该基底膜有望用于实际应用当中。
A large-area and dense two-dimensional(2 D) surface-enhanced Raman scattering(SERS) arrays was fabricated by liquid-liquid interface self-assembly method at the hexane/water two-phase interface.With hexane as the oil phase,the gold nanorods(Au NRs) after aptamer replacement were the aqueous phase.The addition of ethanol quickly induced the Au NRs to self-assemble the 2 D-SERS arrays at the oil-water interface.Displacement of cetyltrimethylammonium bromide(CTAB) on the surface of Au NRs with polyvinylpyrrolidone(PVP) not only effectively reduces the surface charge of Au NRs,but also reduces the repulsive force between the nanoparticles.Then,the crystal violet(CV) was used as the Raman probe molecule to investigate the arrays with high sensitivity and good repeatability,and the plasticizer BBP was detected.The results showed that the detection limit of BBP of the arrays reached 10~(-8) mol/L,and the toxic dose(1.3 mg/kg) of BBP added to a commercially available liquor was successfully detected.Therefore,the arrays is expected to be used in practical applications.
A large-area and dense two-dimensional(2 D) surface-enhanced Raman scattering(SERS) arrays was fabricated by liquid-liquid interface self-assembly method at the hexane/water two-phase interface.With hexane as the oil phase,the gold nanorods(Au NRs) after aptamer replacement were the aqueous phase.The addition of ethanol quickly induced the Au NRs to self-assemble the 2 D-SERS arrays at the oil-water interface.Displacement of cetyltrimethylammonium bromide(CTAB) on the surface of Au NRs with polyvinylpyrrolidone(PVP) not only effectively reduces the surface charge of Au NRs,but also reduces the repulsive force between the nanoparticles.Then,the crystal violet(CV) was used as the Raman probe molecule to investigate the arrays with high sensitivity and good repeatability,and the plasticizer BBP was detected.The results showed that the detection limit of BBP of the arrays reached 10~(-8) mol/L,and the toxic dose(1.3 mg/kg) of BBP added to a commercially available liquor was successfully detected.Therefore,the arrays is expected to be used in practical applications.
引文
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[2] Vaccher C,Decaudin B,Sautou V,et al.Analysis of non-phthalates plasticizers on porous graphitic carbon by supercritical fluid chromatography using evaporative light scattering detection[J].J Chromatogr A,2014,1359:277-286.
[3] Ciallamay D,Zheng X,Weber K,et al.Recent progress in surface-enhanced Raman spectroscopy for biological and biomedical applications:from cells to clinics[J].Chemical Society Reviews,2017,46(13):3945-3961.
[4] Yaseen T,Sun D,Cheng J.Raman imaging for food quality and safety evaluation:Fundamentals and applications[J].Trends in Food Science & Technology,2017,62:177-189.
[5] Wang J,Zhang L,Xin D,et al.Dispersive micro-solid-phase extraction based on decanoic acid coated-Fe3O4 nanoparticles for HPLC analysis of phthalate esters in liquor samples[J].Journal of Food Science,2015,80(11):C2452-C2458.
[6] Liou S,Yang G,Wang C,et al.Monitoring of PAEMs and beta-agonists in urine for a small group of experimental subjects and PAEs and beta-agonists in drinking water consumed by the same subjects[J].Journal of Hazardous Materials,2014,277(4):169-179.
[7] Gong C,Ou X,Liu S,et al.A molecular imprinting-based multifunctional chemosensor for phthalate esters[J].Dyes & Pigments,2016,137:499-506.
[8] Park K,Drummy L,Wadams R,et al.Growth mechanism of gold nanorods[J].Chemistry of Materials,2013,25(4):555-563.
[9] Lee Y,Lee C,Tan B,et al.Using the Langmuir-Schaefer technique to fabricate large-area dense SERS-active Au nanoprism monolayer films[J].Nanoscale,2013,5(14):6404-6412.
[10] 宗慎飞,王著元,杨晶,等.聚合物电解质包裹金核银壳纳米棒制备双模式光学细胞成像探针[J].科学通报,2013,58(7):601-607.
[11] Joo S,Han S,Han H,et al.Adsorption and stability of phthalic acid on a colloidal silver surface:Surface-enhanced Raman scattering study[J].Journal of Raman Spectroscopy,2015,31(3):145-150.