金叶龟甲冬青组培快繁技术的研究
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摘要
以金叶龟甲冬青(Ilex crenata)幼嫩茎段为外植体,设置不同的腋芽诱导培养基、增殖培养基、壮苗及生根培养基进行对比研究,建立金叶龟甲冬青组培快繁体系。结果表明,采用培养基MS+ZT 0.5～1.0 mg/L+NAA 0.1 mg/L进行初代培养,腋芽诱导率达95%左右;利用培养基MS+ZT 0.5 mg/L+NAA 0.2 mg/L,丛生芽增殖系数高,幼苗生长健壮且色泽深绿;丛生芽接种于MS+ZT 0.5 mg/L+NAA 0.2 mg/L+蔗糖30 g/L培养基进行壮苗培养,幼苗高度可达4.9 cm,生长健壮,色泽深绿;适宜的生根培养基配方1/2MS+IBA 0.1 mg/L+NAA 0.5 mg/L+活性炭3.0 g/L,生根率100%,根系生长健壮,色泽白色;经生根的试管苗移栽于园土(V)∶蛭石(V)∶泥炭(V)=2∶1∶1混合基质,加以合理管理,成活率可达95%。
To establish an efficient tissue culture system for rapidly propagating, taking young stem section of Ilex crenata ender as explant, different bud-induction medium, proliferation medium, robust seedling medium and rooting medium were conducted. The results showed that the optimal bud-induction medium was MS+ZT 0.5～1.0 mg/L+NAA 0.1 mg/L and the induction rate of axillary bud was about 95%; The optimal medium for proliferation was MS+ZT 0.5 mg/L+NAA 0.2 mg/L,multiplication coefficient was high, seedling growth was strong with thick green; The optimal robust seedling medium was MS+ZT0.5 mg/L+NAA 0.2 mg/L+sugar 30 g/L, the height of seedling reached 4.9 cm; The optimal rooting medium was 1/2 MS+IBA0.1 mg/L+NAA 0.5 mg/L+active-carbon 3.0 g/L, and the roots were strong and white in color, the rooting rate was 100%;The survival percentage of plantlets could be up to 95% through transplanted in the substrate containing garden soil, vermiculite and peat(2∶1∶1,V/V/V).
To establish an efficient tissue culture system for rapidly propagating, taking young stem section of Ilex crenata ender as explant, different bud-induction medium, proliferation medium, robust seedling medium and rooting medium were conducted. The results showed that the optimal bud-induction medium was MS+ZT 0.5～1.0 mg/L+NAA 0.1 mg/L and the induction rate of axillary bud was about 95%; The optimal medium for proliferation was MS+ZT 0.5 mg/L+NAA 0.2 mg/L,multiplication coefficient was high, seedling growth was strong with thick green; The optimal robust seedling medium was MS+ZT0.5 mg/L+NAA 0.2 mg/L+sugar 30 g/L, the height of seedling reached 4.9 cm; The optimal rooting medium was 1/2 MS+IBA0.1 mg/L+NAA 0.5 mg/L+active-carbon 3.0 g/L, and the roots were strong and white in color, the rooting rate was 100%;The survival percentage of plantlets could be up to 95% through transplanted in the substrate containing garden soil, vermiculite and peat(2∶1∶1,V/V/V).
引文
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[15]金香花,郎贤波,李美兰,等.活性炭、MS浓度及生长素对树莓试管苗生根及生长的影响[J].延边大学农学学报,2015,1(37):31-34.
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[17]张素勤,邹志荣,耿广东,等.活性炭对非洲菊组培苗的生根诱导和移栽基质的筛选[J].北方园艺,2008,31(5):207-208.
[2]朱志国.金叶日本冬青愈伤组织诱导及分化的研究[J].安徽农业科学,2007,35(9):2569-2570.
[3]梁慧敏,夏阳.日本龟甲冬青茎段再生快繁体系的建立[J].江苏农业科学,2011,39(5):65-66.
[4]李登中.金叶日本冬青的组织培养与快速繁殖[J].植物生理学通讯,2004,40(5):592.
[5]史云光,朱艳,徐招娣.金叶龟甲冬青袋式组培应用效果试验初报[J].江苏林业科技,2010,37(3):19-21.
[6]张楠,曹后男,宗成文,等.大果黑果枸杞组培快繁技术体系的研究[J].辽宁林业科技,2016(1):22-24.
[7]刘晓娜,马俊莲,张子德,等.上西早生甜柿离体快繁技术研究[J].河北农业大学学报,2004,27(1):61-63.
[8]孟志霞,郭顺星,于雪梅,等.植物生长调节剂对福建金线莲丛生芽增殖的影响[J].中国药学杂志,2008,43(23):1777-1780.
[9] JIN C,CAO H N,ZONG C W,et al. Research on tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz[J].Agricultural Science&Technology,2011,12(11):1585-1588.
[10]程广有,唐晓杰.苦参组培快繁技术体系的初步研究[J].西北植物学报,2007,27(5):1026-1029.
[11]闫斌,潘超美,何洁,等.穿心莲组培苗的壮苗生根与移栽基质研究[J].时珍国医国药,2016,27(7):1730-1732.
[12]李云海,陈丽华,陶仁艳,等.马铃薯试管苗壮苗培养基的筛选[J].现代农业科技,2012(22):65-66.
[13]魏梅娟,李雪,叶清梅,等.铁皮石斛组培苗生长的影响因素研究[J].北方园艺,2011(2):146-148.
[14]朱志国.金叶日本冬青组织培养研究[D].南京:南京农业大学,2006.
[15]金香花,郎贤波,李美兰,等.活性炭、MS浓度及生长素对树莓试管苗生根及生长的影响[J].延边大学农学学报,2015,1(37):31-34.
[16]陈雄伟,邵玲,梁廉,等.活性炭对鼎湖山紫背天葵组培苗生根的影响[J].中药材,2012(9):1369-1373.
[17]张素勤,邹志荣,耿广东,等.活性炭对非洲菊组培苗的生根诱导和移栽基质的筛选[J].北方园艺,2008,31(5):207-208.