碳量子点荧光探针制备及对铬(Ⅵ)的检测应用
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摘要
以生物质基碳源一步水热法合成碳量子点(CQDs)。通过电镜、荧光光谱、红外光谱等表征,制备良好荧光性能的CQDs;探讨Cr(Ⅵ)对CQDs的荧光猝灭效应,建立荧光检测Cr(Ⅵ)离子浓度的方法。结果表明CQDs的平均尺寸在3～5nm,荧光检测显示332nm处有最大紫外吸收特征峰,在440nm处有强荧光发射峰。在pH 6.0的体系中CQDs浓度18.72μg·mL-1(以碳计),室温25℃与Cr(Ⅵ)反应30min猝灭稳定。低浓度Cr(Ⅵ)离子与体系的荧光猝灭程度呈良好的线性关系,可开展深入研究。
In this paper,the carbon quantum dots(CQDs)were firstly synthesized by one-step hydrothermal method using biochemical substances as the carbon source.The CQDs were characterized by electron microscopy,fluorescence spectrometry and Fourier transform infrared spectrometry.The properties characterization demonstrated that CQDs whose size was about 3.5 nm had excellent fluorescence performance.In the buffer solution at pH=6.0,fluorescence quenching turned to be stable after 18.72μg·mL-1 CQDs(in carbon)reacted with Cr(VI)at 25℃for 30 min.The system was determined at the excitation wavelength of 332 nm and the emission wavelength of 440 nm.The results showed that the concentration of Cr(VI)had a good linear relationship with the fluorescence quenching degree of the system,and more in-depth study needs to be developed.
In this paper,the carbon quantum dots(CQDs)were firstly synthesized by one-step hydrothermal method using biochemical substances as the carbon source.The CQDs were characterized by electron microscopy,fluorescence spectrometry and Fourier transform infrared spectrometry.The properties characterization demonstrated that CQDs whose size was about 3.5 nm had excellent fluorescence performance.In the buffer solution at pH=6.0,fluorescence quenching turned to be stable after 18.72μg·mL-1 CQDs(in carbon)reacted with Cr(VI)at 25℃for 30 min.The system was determined at the excitation wavelength of 332 nm and the emission wavelength of 440 nm.The results showed that the concentration of Cr(VI)had a good linear relationship with the fluorescence quenching degree of the system,and more in-depth study needs to be developed.
引文
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[2]林振华,唐志姣,胡玉玲,等.贵金属纳米粒子/碳量子点复合材料的制备及应用[J].食品安全质量检测学报,2016,7(10):3865-3871.
[3]张静姝,田磊,发光碳量子点的合成与毒性[J].应用化工,2013,42(8):1508-1512.
[4]王月,吴文婷,吴明铂,等.可视化黄色荧光石油焦基碳量子点高效检测Cu 2+[J].新型碳材料,2015,30(6):550-559.
[5]胥月,汤纯静,黄宏,等.荧光碳量子点的绿色合成及高灵敏高选择性检测汞离子[J].分析化学,2014,42(9):1252-1258.
[6]张文宇,常青,周雨锋,等.一步合成硫,氮共掺杂的碳量子点及其在Fe 3+检测中的应用[J].发光学报,2016(4):410-415.
[7]李想,刘学达,于金英,等.水热法制备荧光碳量子点的研究[J].化学工程师,2016(7):22-24.
[8]周乐山,史雪严,杨梦娇,等.基于L-苯丙氨酸一步水热合成碳量子点及其对Cu2+的检测[J].南京师大学报:自然科学版,2016,39(2):147-148.
[9]周婷婷,王震震,马荣萱,等.铬(Ⅵ)的碳量子点荧光猝灭效应及其应用[J].冶金分析,2016,36(12):59-64.
[10]荣杰峰,许美珠,吴洪成,等.基于石墨烯量子点的检测地表水中六价铬的荧光传感器[J].分析试验室,2017,36(1):51-55.
[11]邓小燕,李佳渝,谭克俊.基于碳量子点荧光分光光度法检测叶酸[J].分析化学,2014,42(4):542-546.