人参亲环素基因表达载体构建及其在拟南芥中的抗盐活性分析
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摘要
为研究人参亲还素基因的抗盐活性,为该基因在人参抗逆育种方面的应用提供参考,通过植物转基因技术和外施不同浓度Na Cl的方法获得阳性拟南芥植株,研究了不同植株类型不同盐浓度下的种子萌发率、植株生存率、植株主根长、植株分支数等相关指标。结果表明:在盐胁迫作用下转基因拟南芥种子萌发率高于野生型拟南芥;在盐胁迫作用下转基因拟南芥植株生存率显著高于野生型拟南芥;在盐胁迫作用下转基因拟南芥植株主根长大于野生型拟南芥;在盐胁迫作用下转基因拟南芥植株分支数与野生型拟南芥植株分支数没有明显差异。可见人参亲还素基因提高了转基因拟南芥抵御高盐胁迫的能力。
In order to study the salt-tolerant activity of Ginseng Redoxin gene and provide reference for its application in stress-resistant breeding of Ginseng,positive Arabidopsis thaliana plants were obtained by plant transformation technology and NaCl application at different concentrations,and seed germination rate,plant survival rate and plant growth were used to investigate different salt concentrations and different plant types. The main root length,branch number and other related indicators. The results show that the seed germination rate of transgenic Arabidopsis is higher than that of wild Arabidopsis under salt stress; the survival rate of transgenic Arabidopsis is significantly higher than that of wild Arabidopsis under salt stress; the main roots of transgenic Arabidopsis grew larger than that of wild Arabidopsis under salt stress; and the transgenic Arabidopsis under salt stress. There is no significant difference in the number of branches between Arabidopsis thaliana and wild type Arabidopsis thaliana. It is concluded that the ginsenoside gene improves the ability of transgenic Arabidopsis thaliana to resist high salt stress.
In order to study the salt-tolerant activity of Ginseng Redoxin gene and provide reference for its application in stress-resistant breeding of Ginseng,positive Arabidopsis thaliana plants were obtained by plant transformation technology and NaCl application at different concentrations,and seed germination rate,plant survival rate and plant growth were used to investigate different salt concentrations and different plant types. The main root length,branch number and other related indicators. The results show that the seed germination rate of transgenic Arabidopsis is higher than that of wild Arabidopsis under salt stress; the survival rate of transgenic Arabidopsis is significantly higher than that of wild Arabidopsis under salt stress; the main roots of transgenic Arabidopsis grew larger than that of wild Arabidopsis under salt stress; and the transgenic Arabidopsis under salt stress. There is no significant difference in the number of branches between Arabidopsis thaliana and wild type Arabidopsis thaliana. It is concluded that the ginsenoside gene improves the ability of transgenic Arabidopsis thaliana to resist high salt stress.
引文
1 Pliyev B K,Gurvits B Y. Peptidyl-prolyl cis-trans isomerases:Structure and functions.[J]. Biochemistry,1999,64(7):738-751
2 Lang K,Schmid F X,Fischer G. Catalysis of protein folding by prolyl isomerase[J]. Nature,1987,329:268-270
3 Schonbrunner E R,Mayer S I,Tropschug M,et al. Catalysis of protein folding by cyclophilins from different species[J]. Journal of Biological Chemistry,1991,266(6):3630-3635
4 Gasser C S,Gunning D A,Budelier K A,et al. Structure and expression of cytosolic cyclophilin/peptidyl-prolyl cis-trans isomerase of higher plants and production of active tomato cyclophilin in Escherichia coli[J]. Proceedings of the National Academy of Sciences of the United States of America,1990,87(24):9519-9523
5 Yu L,Niu J,Ma Z,et al. Cloning,characterization and chromosome localization of two powdery mildew resistance-related gene sequences from wheat[J]. Acta Botanica Sinica,2002,44(12):1438-1444
6 Lee J R,Park S,Kim J,et al. Molecular and functional characterization of a cyclophilin with antifungal activity from Chinese cabbage[J]. Biochemical and Biophysical Research Communications,2007,353(3):672-678
7 Sharma AD,Kaur P. Combined effect of drought stress and heat shock on cyclophilin protein expression in Triticum aestivum[J]. Gen Appl Plant Physiol,2009,35:88-92
8 Oh K C,Ivanchenko M G,White T J,et al. The diageotropica gene of tomato encodes a cyclophilin:A novel player in auxin signaling[J]. Planta,2006,224(1):133-144
9 Ambrosone A,Batelli G,Nurcato R,et al. The Arabidopsis RNAbinding protein AtRGGA regulates tolerance to salt and drought stress[J]. Plant Physiology,2015,168(1):292-306
10 刘树明. Na Cl胁迫对19种园林植物存活率及生长状态的影响——以福建福州市平潭为研究地[J].中国园艺文摘,2017,33(4):19-21Liu Shuming. Effects of Na Cl stress on the survival rate and growth status of 19 garden plants in pingtan,Fuzhou,Fujian Province[J].Chinese Horticultural Abstract,2017,33(4):19-21
2 Lang K,Schmid F X,Fischer G. Catalysis of protein folding by prolyl isomerase[J]. Nature,1987,329:268-270
3 Schonbrunner E R,Mayer S I,Tropschug M,et al. Catalysis of protein folding by cyclophilins from different species[J]. Journal of Biological Chemistry,1991,266(6):3630-3635
4 Gasser C S,Gunning D A,Budelier K A,et al. Structure and expression of cytosolic cyclophilin/peptidyl-prolyl cis-trans isomerase of higher plants and production of active tomato cyclophilin in Escherichia coli[J]. Proceedings of the National Academy of Sciences of the United States of America,1990,87(24):9519-9523
5 Yu L,Niu J,Ma Z,et al. Cloning,characterization and chromosome localization of two powdery mildew resistance-related gene sequences from wheat[J]. Acta Botanica Sinica,2002,44(12):1438-1444
6 Lee J R,Park S,Kim J,et al. Molecular and functional characterization of a cyclophilin with antifungal activity from Chinese cabbage[J]. Biochemical and Biophysical Research Communications,2007,353(3):672-678
7 Sharma AD,Kaur P. Combined effect of drought stress and heat shock on cyclophilin protein expression in Triticum aestivum[J]. Gen Appl Plant Physiol,2009,35:88-92
8 Oh K C,Ivanchenko M G,White T J,et al. The diageotropica gene of tomato encodes a cyclophilin:A novel player in auxin signaling[J]. Planta,2006,224(1):133-144
9 Ambrosone A,Batelli G,Nurcato R,et al. The Arabidopsis RNAbinding protein AtRGGA regulates tolerance to salt and drought stress[J]. Plant Physiology,2015,168(1):292-306
10 刘树明. Na Cl胁迫对19种园林植物存活率及生长状态的影响——以福建福州市平潭为研究地[J].中国园艺文摘,2017,33(4):19-21Liu Shuming. Effects of Na Cl stress on the survival rate and growth status of 19 garden plants in pingtan,Fuzhou,Fujian Province[J].Chinese Horticultural Abstract,2017,33(4):19-21