白桦BpTCPs基因家族生物信息学及时空表达分析
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摘要
【目的】通过研究白桦TCP转录因子的序列特征及其在不同时期组织中的表达规律,为揭示BpTCPs在白桦生长发育中的作用提供证据。【方法】依据白桦45个转录组测序结果,共获得15条TCPs基因。利用生物信息学方法对TCPs基因进行了全面分析,采用实时荧光定量PCR技术分析基因在不同组织中的表达模式。【结果】生物信息学分析发现,15条BpTCPs均含有1个高度保守的b HLH结构域,系统进化分析发现15条白桦TCP分属于TCP蛋白的两大类;qRT-PCR分析结果显示,自5月到9月的生长阶段中,顶芽中BpTCPs的表达水平呈现不同程度升高的变化趋势;茎中BpTCP3在整个生长季上调表达;BpTCP4、BpTCP10及BpTCP7、BpTCP8分别在越冬后的雌花及雄花中上调表达。【结论】明确了BpTCPs基因的功能及揭示其参与调控植物生长的分子机制奠定基础。
【Objective】To analyze the sequence features and gene expression patterns of TCP transcription factors from Betula platyphylla Suk. in different tissues and time points. 【Method】Based on 45 birch transcriptome databases,15 BpTCP genes were identified and the sequence features and gene expression at different time points and in different tissues( leaves,terminal buds,stems,male inflorescences and female inflorescences) were analyzed.【Result】Bioinformatic analysis showed that all 15 BpTCPs contained a highly conserved b HLH domain. Phylogenetic analysis showed that the 15 birch TCP proteins belonged to two major categories.The qRT-PCR results suggested that the expression levels of the 15 BpTCP genes changed significantly in terminal buds and that most BpTCP genes were up-regulated from May to September. In stems,BpTCP3 exhibited an increasing pattern during the full growth period; BpTCP4,BpTCP10,BpTCP7 and BpTCP8 were up-regulated in male and female inflorescences after winter.【Conclusion】This study may provide information for further research on the functional characteristics of BpTCPs gene and the molecular mechanism( s)involved in the regulation of plant growth.
【Objective】To analyze the sequence features and gene expression patterns of TCP transcription factors from Betula platyphylla Suk. in different tissues and time points. 【Method】Based on 45 birch transcriptome databases,15 BpTCP genes were identified and the sequence features and gene expression at different time points and in different tissues( leaves,terminal buds,stems,male inflorescences and female inflorescences) were analyzed.【Result】Bioinformatic analysis showed that all 15 BpTCPs contained a highly conserved b HLH domain. Phylogenetic analysis showed that the 15 birch TCP proteins belonged to two major categories.The qRT-PCR results suggested that the expression levels of the 15 BpTCP genes changed significantly in terminal buds and that most BpTCP genes were up-regulated from May to September. In stems,BpTCP3 exhibited an increasing pattern during the full growth period; BpTCP4,BpTCP10,BpTCP7 and BpTCP8 were up-regulated in male and female inflorescences after winter.【Conclusion】This study may provide information for further research on the functional characteristics of BpTCPs gene and the molecular mechanism( s)involved in the regulation of plant growth.
引文
[1]DOEBLEY J,STEC A,HUBBARD L.The evolution of apical dominance in maize[J].Nature,1997,386(6624):485-488.DOI:10.1038/386485a0.
[2]LUO D,CARPENTER R,VINCENT C,et al.Origin of floral asymmetry in Antirrhinum[J].Nature,1996,383(6603):794-799.DOI:10.1038/383794a0.
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[4]RESENTINI F,FELIPO-BENAVENT A,COLOMBO L,et al.TCP14 and TCP15 mediate the promotion of seed germination by gibberellins in Arabidopsis thaliana[J].Mol Plant,2015,8(3):482-485.DOI:10.1016/j.molp.2014.11.018.
[5]HERVéC,DABOS P,BARDET C,et al.In vivo interference with At TCP20 function induces severe plant growth alterations and deregulates the expression of many genes important for development[J].Plant Physiol,2009,149(3):1462-1477.DOI:10.1104/pp.108.126136.
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[7]KOYAMA T,FURUTANI M,TASAKA M,et al.TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis[J].Plant Cell,2007,19(2):473-484.DOI:10.1105/tpc.106.044792.
[8]SARVEPALLI K,NATH U.Interaction of TCP4-mediated growth module with phytohormones[J].Plant Signal Behav,2011,6(10):1440-1443.DOI:10.4161/psb.6.10.17097.
[9]NAVAUD O,DABOS P,CARNUS E,et al.TCP transcription factors predate the emergence of land plants[J].J Mol Evol,2007,65(1):23-33.DOI:10.1007/s00239-006-0174-z.
[10]刘文文,李文学.植物b HLH转录因子研究进展[J].植物技术进展,2013,3(1):7-11.LIU W W,LI W X.Progress of plant b HLH transcription factor[J].Current Biotechnology,2013,3(1):7-11.
[11]CUBAS P,LAUTER N,DOEBLEY J,et al.The TCP domain:a motif found in proteins regulating plant growth and development[J].The Plant Journal,1999,18(2):215-222.DOI:10.1046/j.1365-313X.1999.00444.x.
[12]FRANCIS A,DHAKA N,BAKSHI M,et al.Comparative phylogenomic analysis provides insights into TCP gene functions in Sorghum[J].Sci Rep,2016,5(6):38488.DOI:10.1038/srep38488.
[13]EFRONI I,BLUM E,GOLDSHMIDT A,et al.A protracted and dynamic maturation schedule underlies Arabidopsis leaf development[J].Plant Cell,2008,20(9):2293-2306.DOI:10.1105/tpc.107.057521.
[14]KOYAMA T,SATO F,OHME-TAKAGI M.A role of TCP1 in the longitudinal elongation of leaves in Arabidopsis[J].Biosci Biotechnol Biochem,2010,74(10):2145-2147.DOI:10.1271/bbb.100442.
[15]VIOLA I L,MANASSERO N G,RIPOLL R,et al.The Arabidopsis class I TCP transcription factor At TCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain[J].Biochem J,2011,435(1):143-155.DOI:10.1042/BJ20101019.
[16]TAKEDA T,AMANO K,OHTO M,et al.RNA interference of the Arabidopsis putative transcription factor TCP16 gene results in abortion of early pollen development[J].Plant Mol Biol,2006,61(1-2):165-177.DOI:10.1007/s11103-006-6265-9.
[17]UBERTI-MANASSERO N G,COSCUETA E R,GONZALEZ D H,et al.Expression of a repressor form of the Arabidopsis transcription factor TCP16 induces the formation of ectopic meristems[J].Plant Physiol Biochem,2016,108:57-62.DOI:10.1016/j.plaphy.2016.06.031.
[18]BREUIL-BROYER S,MOREL P,de ALMEIDA-ENGLER J,et al.High-resolution boundary analysis during Arabidopsis thaliana flower development[J].Plant J,2004,38(1):182-192.DOI:10.1111/j.1365-313X.2004.02026.x.
[19]LAUFS P,PEAUCELLE A,MORIN H,et al.MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems[J].Development,2004,131(7):4311-4322.DOI:10.1242/dev.01320.
[2]LUO D,CARPENTER R,VINCENT C,et al.Origin of floral asymmetry in Antirrhinum[J].Nature,1996,383(6603):794-799.DOI:10.1038/383794a0.
[3]KOSUGI S,OHASHI Y.PCF1 and PCF2 specifically bind to cis elements in the rice PROLIFERATING CELL NUCLEAR ANTIGEN gene[J].Plant Cell,1997,9(9):1607-1619.DOI:10.1105/tpc.9.9.1607.
[4]RESENTINI F,FELIPO-BENAVENT A,COLOMBO L,et al.TCP14 and TCP15 mediate the promotion of seed germination by gibberellins in Arabidopsis thaliana[J].Mol Plant,2015,8(3):482-485.DOI:10.1016/j.molp.2014.11.018.
[5]HERVéC,DABOS P,BARDET C,et al.In vivo interference with At TCP20 function induces severe plant growth alterations and deregulates the expression of many genes important for development[J].Plant Physiol,2009,149(3):1462-1477.DOI:10.1104/pp.108.126136.
[6]PRUNEDA-PAZ J L,BRETON G,PARA A,et al.A functional genomics approach reveals CHE as a component of the Arabidopsis circadian clock[J].Science,2009,323(5920):1481-1485.DOI:10.1126/science.1167206.
[7]KOYAMA T,FURUTANI M,TASAKA M,et al.TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis[J].Plant Cell,2007,19(2):473-484.DOI:10.1105/tpc.106.044792.
[8]SARVEPALLI K,NATH U.Interaction of TCP4-mediated growth module with phytohormones[J].Plant Signal Behav,2011,6(10):1440-1443.DOI:10.4161/psb.6.10.17097.
[9]NAVAUD O,DABOS P,CARNUS E,et al.TCP transcription factors predate the emergence of land plants[J].J Mol Evol,2007,65(1):23-33.DOI:10.1007/s00239-006-0174-z.
[10]刘文文,李文学.植物b HLH转录因子研究进展[J].植物技术进展,2013,3(1):7-11.LIU W W,LI W X.Progress of plant b HLH transcription factor[J].Current Biotechnology,2013,3(1):7-11.
[11]CUBAS P,LAUTER N,DOEBLEY J,et al.The TCP domain:a motif found in proteins regulating plant growth and development[J].The Plant Journal,1999,18(2):215-222.DOI:10.1046/j.1365-313X.1999.00444.x.
[12]FRANCIS A,DHAKA N,BAKSHI M,et al.Comparative phylogenomic analysis provides insights into TCP gene functions in Sorghum[J].Sci Rep,2016,5(6):38488.DOI:10.1038/srep38488.
[13]EFRONI I,BLUM E,GOLDSHMIDT A,et al.A protracted and dynamic maturation schedule underlies Arabidopsis leaf development[J].Plant Cell,2008,20(9):2293-2306.DOI:10.1105/tpc.107.057521.
[14]KOYAMA T,SATO F,OHME-TAKAGI M.A role of TCP1 in the longitudinal elongation of leaves in Arabidopsis[J].Biosci Biotechnol Biochem,2010,74(10):2145-2147.DOI:10.1271/bbb.100442.
[15]VIOLA I L,MANASSERO N G,RIPOLL R,et al.The Arabidopsis class I TCP transcription factor At TCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain[J].Biochem J,2011,435(1):143-155.DOI:10.1042/BJ20101019.
[16]TAKEDA T,AMANO K,OHTO M,et al.RNA interference of the Arabidopsis putative transcription factor TCP16 gene results in abortion of early pollen development[J].Plant Mol Biol,2006,61(1-2):165-177.DOI:10.1007/s11103-006-6265-9.
[17]UBERTI-MANASSERO N G,COSCUETA E R,GONZALEZ D H,et al.Expression of a repressor form of the Arabidopsis transcription factor TCP16 induces the formation of ectopic meristems[J].Plant Physiol Biochem,2016,108:57-62.DOI:10.1016/j.plaphy.2016.06.031.
[18]BREUIL-BROYER S,MOREL P,de ALMEIDA-ENGLER J,et al.High-resolution boundary analysis during Arabidopsis thaliana flower development[J].Plant J,2004,38(1):182-192.DOI:10.1111/j.1365-313X.2004.02026.x.
[19]LAUFS P,PEAUCELLE A,MORIN H,et al.MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems[J].Development,2004,131(7):4311-4322.DOI:10.1242/dev.01320.