锤胁跷蝽触角转录组分析
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摘要
为了获得锤胁跷蝽(Yemma signatus Hsiao)触角基因信息,本研究使用Illumina HiSeq TM 4 000高通量测序技术对锤胁跷蝽成虫触角进行转录组测序和生物学信息分析。共获得61 080 938条clean reads,包括9.16 G (GenBank登录号:SRR3348966)。拼接115 491条unigenes,平均长度为578 bp,N50为863 bp。在7大数据库中注释到46 224条unigenes,其中在NR数据库中注释的基因最多,为32 842 (28.43%)条,与内华达古白蚁(Zootermopsis nevadensis)相似度最高(9.0%)。转录组数据分析鉴定出125个与嗅觉相关的基因,其中66个嗅觉受体、11个味觉受体、1个离子型受体、3个感觉神经元膜蛋白、30个气味结合蛋白和14个化学感受蛋白基因。本研究首次获得锤胁跷蝽触角转录组数据,并鉴定出嗅觉相关基因,本研究为今后利用嗅觉基因靶标防治害虫提供了分子生物学信息数据。
In order to obtain the gene information of Yemma signatus Hsiao antenna, Y. signatus adult antenna was carried out transcriptome sequencing and bioinformatics analysis using Illumina HiSeqTM4 000 high-throughput sequencing technology. In total, 61 080 938 clean reads with 9.16 G were obtained(GenBank accession No.:SRR3348966). 115 491 unigenes with a mean length of 578 bp and an N50 length of 863 bp were spliced. 46 224 unigenes were annotated in seven databases. Most unigenes were annotated in NR database, for 32 842(28.43%),which had the highest similarity(9.0%) to those of Zootermopsis nevadensis. Analysis of the transcriptome data identified 125 olfaction-related genes, including 66 for olfactory receptors(ORs), 11 for gustatory receptors(GRs),1 for ionotropic receptors(IRs), 3 for sensory neuron membrane proteins(SNMPs), 30 for odorant-binding proteins(OBPs) and 14 for chemosensory proteins(CSPs). This study acquired the transcriptom data of Y. signatus and identified olfaction-related genes for the first time, which could provide information data of molecular biology for future prevention and control of pests using olfaction-related gene targeting.
In order to obtain the gene information of Yemma signatus Hsiao antenna, Y. signatus adult antenna was carried out transcriptome sequencing and bioinformatics analysis using Illumina HiSeqTM4 000 high-throughput sequencing technology. In total, 61 080 938 clean reads with 9.16 G were obtained(GenBank accession No.:SRR3348966). 115 491 unigenes with a mean length of 578 bp and an N50 length of 863 bp were spliced. 46 224 unigenes were annotated in seven databases. Most unigenes were annotated in NR database, for 32 842(28.43%),which had the highest similarity(9.0%) to those of Zootermopsis nevadensis. Analysis of the transcriptome data identified 125 olfaction-related genes, including 66 for olfactory receptors(ORs), 11 for gustatory receptors(GRs),1 for ionotropic receptors(IRs), 3 for sensory neuron membrane proteins(SNMPs), 30 for odorant-binding proteins(OBPs) and 14 for chemosensory proteins(CSPs). This study acquired the transcriptom data of Y. signatus and identified olfaction-related genes for the first time, which could provide information data of molecular biology for future prevention and control of pests using olfaction-related gene targeting.
引文
An X.K.,Sun L.,Liu H.W.,Liu D.F.,Ding Y.X.,Li L.M.,Zhang Y.J.,and Guo Y.Y.,2016,Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum(Meyer-Dür),Sci.Rep-UK,6:37870
Becher P.G.,Flick G.,Rozpedowska E.,Schmidt A.,Hagman A.,Lebreton S.,Larsson M.C.,Hansson B.S.,Pi觢kur J.,Witzgall P.,and Bengtsson M.,2012,Yeast,not fruit volatiles mediate Drosophila melanogaster attraction,oviposition and development,Funct.Ecol.,26(4):822-828
Benton R.,Vannice K.S.,Gomez-Diaz C.,and Vosshall L.B.,2009,Variant lonotropic glutamate receptors as chemosensory receptors in Drosophila,Cell,136:149-162
Benton R.,Vannice K.S.,and Vosshall L.B.,2007,An essential role for a CD36-related receptor in pheromone detection in Drosophila,Nature,450:289-293
Birkett M.A.,Agelopoulos N.,Jensen K.M.V.,Jespersen J.B.,Pickett J.A.,Prijs H.J.,Thomas G.,Trapman J.J.,Wadhams L.J.,and Woodcock C.M.,2004,The role of volatile semiochemicals in mediating host location and selection by nuisance and disease-transmitting cattle flies,Med.Vet.Entomol.,18(4):313-322
Clyne P.J.,Warr C.G.,and Carlson J.R.,2000,Candidate taste receptors in Drosophila,Science,287:1830-1834
Clyne P.J.,Warr C.G.,Freeman M.R.,Lessing D.,Kim J.,and Carlson J.R.,1999,A novel family of divergent seven transmembrane proteins:candidate odorant receptors in Drosophila,Neuron,22:327-338
Cui H.H.,Gu S.H.,Zhu X.Q.,Wei Y.,Liu H.W.,Khalid H.D.,Guo Y.Y.,and Zhang Y.J.,2016,Odorant-binding and chemosensory proteins identified in the antennal transcriptome of adelphocoris suturalis jakovlev,Elsevier,(8):1-7
Cui J.X.,and Cai W.Z.,2009,Observations on takeoff behavior of Yemma signatus,Kunchong Zhishi(Chinese Bulletin of Entomology),46(5):783-787(崔建新,彩万志,2009,锤胁跷蝽的起飞行为观察,昆虫知识,46(5):783-787)
Gu S.H.,Wang S.P.,Zhang X.Y.,Wu K.M.,Guo Y.Y.,Zhou J.J.,and Zhang Y.J.,2011,Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus(Goeze),Insect Biochem.Molec.,41:254-263
Jin Y.L.,Cong B.,Wang L.Y.,Zhang H.Y.,and Dong H.,2015,An analysis of the transcriptome of Epacromius coerulipes(Orthoptera:Acrididae),Kunchong Xuebao(Acta Entomology Sinica),58(8):817-825(金永玲,丛斌,王丽艳,张海燕,董辉,2015,大垫尖翅蝗转录组分析,昆虫学报,58(8):817-825)
Kurtovic A.,Widmer A.,and Dickson B.,2007,A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone,Nature,466(7153):542-546
Leal W.S.,2013,Odorant reception in insects:roles of receptors,binding proteins,and degrading enzymes,Annu.Rev.Entomol,58:373-391
Liang L.J.,Miao G.X.,and Xing B.Q.,1992,Study on the predatory function of Yemma signatus,Hebei Linye Keji(The Journd of Hebei Forestry Science and Technology),(2):39-42(粱丽鹃,苗国显,邢宝琴,1992,锤胁跷蝽捕食功能的研究,河北林业科技,(2):39-42)
Meng X.,Hu J.J.,Liu H.,Ouyang G.C.,and Guo M.F.,2016,Analysis of the transcriptome and olfaction-related genes of Conopomorpha sinensis bradley(Lepidoptera:Gracilariidae),Kunchong Xuebao(Acta Entomology Sinica),59(8):823-830(孟翔,胡俊杰,刘慧,欧阳革成,郭明昉,2016,荔枝蒂蛀虫转录组及嗅觉相关基因分析,昆虫学报,59(8):823-830)
Pelosi P.,Iovinella I.,Felicioli A.,and Dani F.R.,2014,Soluble proteins of chemical communication:an overview across arthropods,Front Physiol,5:320
Plettner E.,2002,Insect pheromone olfaction:new targets for the design of species-selective pest control agents,Curr.Med.Chem.,9(10):1075-1085
Ronderos D.S.,and Smith D.P.,2009,Diverse signaling mechanisms mediate volatile odorant detection in Drosophila,Fly(Austin),3:290-297
Steinbrecht R.A.,Laue M.,Maida R.,and Ziegelberger G.,1996,Odorant-binding proteins and their role in the detection of plant odours,Ent.Exp.Appl.,80:15-18
Vogt R.G.and Riddiford L.M.,1981,Pheromone binding and inactivation by moth antennae,Nature,293:161-163
Xiao Y.,Sun L.,Ma X.Y.,Dong K.,Liu H.W.,Wang Q.,Guo Y.Y.,Liu Z.W.,and Zhang Y.J.,2016,Identification and characterization of the distinct expression profiles of candidate chemosensory membrane proteins in the antennal transcriptome of Adelphocoris lineolatus(Goeze),Insect Mol.Biol.,26(1):74-91
Yuan H.B.,Ding Y.X.,Gu S.H.,Sun L.,Zhu X.Q.,Liu H.W.,Dhiloo K.H.,Zhang Y.J.,and Guo Y.Y.,2015,Molecular characterization and expression profiling of odorant-binding proteins in Apolygus lucorum,PLoS One,10(10):e0140562
Zhang Q.L.,and Yuan M.L.,2013,Progress in insect transcriptomics based on the next-generation sequencing technique,Kunchong Xuebao(Acta Entomologica Sinica),56(12):1489-1508(张棋麟,袁明龙,2013,基于新一代测序技术的昆虫转录组学研究进展,昆虫学报,56(12):1489-1508)
Zhang Y.N.,Zhu X.Y.,Zhang Q.,Yin C.Y.,Dong Z.P.,Zuo L.H.,Deng D.G.,Sun L.,and Li X.M.,2016,De novo assembly and characterization of antennal transcriptome reveal chemosensory system in Nysius ericae,J.Asia-Pac.Entomol,19:1077-1087
Becher P.G.,Flick G.,Rozpedowska E.,Schmidt A.,Hagman A.,Lebreton S.,Larsson M.C.,Hansson B.S.,Pi觢kur J.,Witzgall P.,and Bengtsson M.,2012,Yeast,not fruit volatiles mediate Drosophila melanogaster attraction,oviposition and development,Funct.Ecol.,26(4):822-828
Benton R.,Vannice K.S.,Gomez-Diaz C.,and Vosshall L.B.,2009,Variant lonotropic glutamate receptors as chemosensory receptors in Drosophila,Cell,136:149-162
Benton R.,Vannice K.S.,and Vosshall L.B.,2007,An essential role for a CD36-related receptor in pheromone detection in Drosophila,Nature,450:289-293
Birkett M.A.,Agelopoulos N.,Jensen K.M.V.,Jespersen J.B.,Pickett J.A.,Prijs H.J.,Thomas G.,Trapman J.J.,Wadhams L.J.,and Woodcock C.M.,2004,The role of volatile semiochemicals in mediating host location and selection by nuisance and disease-transmitting cattle flies,Med.Vet.Entomol.,18(4):313-322
Clyne P.J.,Warr C.G.,and Carlson J.R.,2000,Candidate taste receptors in Drosophila,Science,287:1830-1834
Clyne P.J.,Warr C.G.,Freeman M.R.,Lessing D.,Kim J.,and Carlson J.R.,1999,A novel family of divergent seven transmembrane proteins:candidate odorant receptors in Drosophila,Neuron,22:327-338
Cui H.H.,Gu S.H.,Zhu X.Q.,Wei Y.,Liu H.W.,Khalid H.D.,Guo Y.Y.,and Zhang Y.J.,2016,Odorant-binding and chemosensory proteins identified in the antennal transcriptome of adelphocoris suturalis jakovlev,Elsevier,(8):1-7
Cui J.X.,and Cai W.Z.,2009,Observations on takeoff behavior of Yemma signatus,Kunchong Zhishi(Chinese Bulletin of Entomology),46(5):783-787(崔建新,彩万志,2009,锤胁跷蝽的起飞行为观察,昆虫知识,46(5):783-787)
Gu S.H.,Wang S.P.,Zhang X.Y.,Wu K.M.,Guo Y.Y.,Zhou J.J.,and Zhang Y.J.,2011,Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus(Goeze),Insect Biochem.Molec.,41:254-263
Jin Y.L.,Cong B.,Wang L.Y.,Zhang H.Y.,and Dong H.,2015,An analysis of the transcriptome of Epacromius coerulipes(Orthoptera:Acrididae),Kunchong Xuebao(Acta Entomology Sinica),58(8):817-825(金永玲,丛斌,王丽艳,张海燕,董辉,2015,大垫尖翅蝗转录组分析,昆虫学报,58(8):817-825)
Kurtovic A.,Widmer A.,and Dickson B.,2007,A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone,Nature,466(7153):542-546
Leal W.S.,2013,Odorant reception in insects:roles of receptors,binding proteins,and degrading enzymes,Annu.Rev.Entomol,58:373-391
Liang L.J.,Miao G.X.,and Xing B.Q.,1992,Study on the predatory function of Yemma signatus,Hebei Linye Keji(The Journd of Hebei Forestry Science and Technology),(2):39-42(粱丽鹃,苗国显,邢宝琴,1992,锤胁跷蝽捕食功能的研究,河北林业科技,(2):39-42)
Meng X.,Hu J.J.,Liu H.,Ouyang G.C.,and Guo M.F.,2016,Analysis of the transcriptome and olfaction-related genes of Conopomorpha sinensis bradley(Lepidoptera:Gracilariidae),Kunchong Xuebao(Acta Entomology Sinica),59(8):823-830(孟翔,胡俊杰,刘慧,欧阳革成,郭明昉,2016,荔枝蒂蛀虫转录组及嗅觉相关基因分析,昆虫学报,59(8):823-830)
Pelosi P.,Iovinella I.,Felicioli A.,and Dani F.R.,2014,Soluble proteins of chemical communication:an overview across arthropods,Front Physiol,5:320
Plettner E.,2002,Insect pheromone olfaction:new targets for the design of species-selective pest control agents,Curr.Med.Chem.,9(10):1075-1085
Ronderos D.S.,and Smith D.P.,2009,Diverse signaling mechanisms mediate volatile odorant detection in Drosophila,Fly(Austin),3:290-297
Steinbrecht R.A.,Laue M.,Maida R.,and Ziegelberger G.,1996,Odorant-binding proteins and their role in the detection of plant odours,Ent.Exp.Appl.,80:15-18
Vogt R.G.and Riddiford L.M.,1981,Pheromone binding and inactivation by moth antennae,Nature,293:161-163
Xiao Y.,Sun L.,Ma X.Y.,Dong K.,Liu H.W.,Wang Q.,Guo Y.Y.,Liu Z.W.,and Zhang Y.J.,2016,Identification and characterization of the distinct expression profiles of candidate chemosensory membrane proteins in the antennal transcriptome of Adelphocoris lineolatus(Goeze),Insect Mol.Biol.,26(1):74-91
Yuan H.B.,Ding Y.X.,Gu S.H.,Sun L.,Zhu X.Q.,Liu H.W.,Dhiloo K.H.,Zhang Y.J.,and Guo Y.Y.,2015,Molecular characterization and expression profiling of odorant-binding proteins in Apolygus lucorum,PLoS One,10(10):e0140562
Zhang Q.L.,and Yuan M.L.,2013,Progress in insect transcriptomics based on the next-generation sequencing technique,Kunchong Xuebao(Acta Entomologica Sinica),56(12):1489-1508(张棋麟,袁明龙,2013,基于新一代测序技术的昆虫转录组学研究进展,昆虫学报,56(12):1489-1508)
Zhang Y.N.,Zhu X.Y.,Zhang Q.,Yin C.Y.,Dong Z.P.,Zuo L.H.,Deng D.G.,Sun L.,and Li X.M.,2016,De novo assembly and characterization of antennal transcriptome reveal chemosensory system in Nysius ericae,J.Asia-Pac.Entomol,19:1077-1087