混合表型急性白血病患者骨髓细胞形态学与流式免疫表型特点
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摘要
目的探讨混合表型急性白血病(MPAL)患者骨髓细胞形态学与流式免疫表型特点,为血液病诊断提供参考。方法收集9例MPAL患者的临床资料,对其骨髓细胞形态学与流式免疫表型特点进行分析。结果9例MPAL患者CD34阳性表达率为77.78%,同期入院的急性髓系单一表型白血病与急性淋系单一表型白血病患者CD34阳性表达率分别为51.74%、60.00%。免疫表型以cMPO、cCD79a、CD3为主。结论 MPAL特点极其复杂,应结合骨髓细胞形态学与流式免疫表型检测结果加以分析,以提高MPAL诊断的准确率。
Objective To evaluate the bone marrow cell morphology and immuno-phenotype characteristics of mixed phenotype acute leukemia(MPAL)patients by ?ow cytometry,and to provide a reference for the diagnosis of leukemia. Methods The clinical data of 9 MPAL patients were collected,and their bone marrow cell morphology and immuno-phenotype characteristics were evaluated. Results The CD34 positive expression rate of 9 MPAL patients was 77.78%. The CD34 positive expression rates of acute myeloid and lymphoid monophenotypic leukemias were 51.74% and 60.00%,respectively. The immuno-phenotypes were mainly expressed in cMPO,c CD79 a and CD3.Conclusions The characteristics of MPAL are complex. The results of bone marrow cell morphology and immunophenotype by ?ow cytometry should be analyzed combinedly in order to improve the diagnostic accuracy of MPAL.
Objective To evaluate the bone marrow cell morphology and immuno-phenotype characteristics of mixed phenotype acute leukemia(MPAL)patients by ?ow cytometry,and to provide a reference for the diagnosis of leukemia. Methods The clinical data of 9 MPAL patients were collected,and their bone marrow cell morphology and immuno-phenotype characteristics were evaluated. Results The CD34 positive expression rate of 9 MPAL patients was 77.78%. The CD34 positive expression rates of acute myeloid and lymphoid monophenotypic leukemias were 51.74% and 60.00%,respectively. The immuno-phenotypes were mainly expressed in cMPO,c CD79 a and CD3.Conclusions The characteristics of MPAL are complex. The results of bone marrow cell morphology and immunophenotype by ?ow cytometry should be analyzed combinedly in order to improve the diagnostic accuracy of MPAL.
引文
[1]ELAINE S,NANCY L H,JAMES W.血液病理学[M].陈刚,李小秋,译.北京:北京科学技术出版社,2013:735-738.
[2]XU X Q,WANG J M,LüS Q,et al.Clinical and biological characteristics of adult biphenotypic acute leukemia in comparison with that of acute myeloid leukemia and acute lymphoblastic leukemia:a case series of a Chinese population[J].Haematologica,2009,94(7):919-927.
[3]KHAN M,SIDDIQI R,NAQVI K.An update on classification,genetics,and clinical approach to mixed phenotype acute leukemia(MPAL)[J].Ann Hematol,2018,97(6):945-953.
[4]鲁广建,宋志善,张群妹,等.CD34抗原在急性双表型白血病的临床研究[J].中国实验血液学杂志,2014,22(5):1226-1229.
[5]BéNéM C,NEBE T,BETTELHEIM P,et al.Immunophenotyping of acute leukemia and lymphoproliferative disorders:a consensus proposal of the European LeukemiaNet Work Package 10[J].Leukemia,2011,25(4):567-574.
[2]XU X Q,WANG J M,LüS Q,et al.Clinical and biological characteristics of adult biphenotypic acute leukemia in comparison with that of acute myeloid leukemia and acute lymphoblastic leukemia:a case series of a Chinese population[J].Haematologica,2009,94(7):919-927.
[3]KHAN M,SIDDIQI R,NAQVI K.An update on classification,genetics,and clinical approach to mixed phenotype acute leukemia(MPAL)[J].Ann Hematol,2018,97(6):945-953.
[4]鲁广建,宋志善,张群妹,等.CD34抗原在急性双表型白血病的临床研究[J].中国实验血液学杂志,2014,22(5):1226-1229.
[5]BéNéM C,NEBE T,BETTELHEIM P,et al.Immunophenotyping of acute leukemia and lymphoproliferative disorders:a consensus proposal of the European LeukemiaNet Work Package 10[J].Leukemia,2011,25(4):567-574.